LOCUS       BBa_K145259             1841 bp    DNA     linear       25-Nov-2024
DEFINITION  MEMORY Part 2
ACCESSION   BBa_K145259
VERSION     BBa_K145259.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=OR3
     misc_feature    11..28
                     /label=OR2
     misc_feature    34..51
                     /label=OR1
     promoter        18..23
                     /label=-35
     promoter        8..13
                     /label=-35
     promoter        30..35
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0053
     promoter        1..54
                     /label=BBa_R0053
     misc_feature    69..72
                     /label=conserved
     RBS             63..76
                     /label=BBa_B0031
     CDS             83..751
                     /label=cI 434
     misc_feature    713..751
                     /label=LVA
     misc_feature    83..751
                     /label=BBa_C0052
     misc_feature    752..776
                     /label=Help:Barcodes
     CDS             83..751
                     /label=BBa_C0052
     stem_loop       796..839
                     /label=stem_loop
     misc_feature    785..864
                     /label=BBa_B0010
     terminator      785..864
                     /label=BBa_B0010
     stem_loop       880..899
                     /label=T7 TE
     misc_feature    906..906
                     /label=stop
     polyA_site      900..913
                     /label=polya
     misc_feature    873..913
                     /label=BBa_B0012
     terminator      873..913
                     /label=BBa_B0012
     misc_feature    63..913
                     /label=BBa_P0152
     promoter        922..964
                     /label=-10
     misc_feature    929..942
                     /label=OR2
     misc_feature    951..964
                     /label=OR1
     promoter        922..927
                     /label=-35
     promoter        923..928
                     /label=-35
     misc_feature    956..958
                     /label=start
     promoter        945..950
                     /label=-10
     misc_feature    922..967
                     /label=BBa_R1052
     promoter        922..967
                     /label=BBa_R1052
     RBS             976..986
                     /label=RBS-4\Weaker
     RBS             982..985
                     /label=RBS
     misc_feature    976..986
                     /label=BBa_B0033
     RBS             976..986
                     /label=BBa_B0033
     CDS             993..1640
                     /label=cII p22
     misc_feature    1641..1673
                     /label=LVA
     misc_feature    1674..1679
                     /label=stop
     misc_feature    993..1679
                     /label=BBa_C0053
     misc_feature    1680..1704
                     /label=Help:Barcodes
     CDS             993..1679
                     /label=BBa_C0053
     stem_loop       1724..1767
                     /label=stem_loop
     misc_feature    1713..1792
                     /label=BBa_B0010
     terminator      1713..1792
                     /label=BBa_B0010
     stem_loop       1808..1827
                     /label=T7 TE
     misc_feature    1834..1834
                     /label=stop
     polyA_site      1828..1841
                     /label=polya
     misc_feature    1801..1841
                     /label=BBa_B0012
     terminator      1801..1841
                     /label=BBa_B0012
     misc_feature    976..1841
                     /label=BBa_P0353
ORIGIN
        1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag
       61 agtcacacag gaaacctact agatgagtat ttcttccagg gtaaaaagca aaagaatcca
      121 gcttggactt aaccaggctg aacttgctca aaaggtgggg actacccagc agtctataga
      181 gcagctcgaa aacggtaaaa ctaagcgacc acgcttttta ccagaacttg cgtcagctct
      241 tggcgtaagt gttgactggc tgctcaatgg cacctctgat tcgaatgtta gatttgttgg
      301 gcacgttgag cccaaaggga aatatccatt gattagcatg gttagagctg gttcgtggtg
      361 tgaagcttgt gaaccctacg atatcaagga cattgatgaa tggtatgaca gtgacgttaa
      421 cttattaggc aatggattct ggctgaaggt tgaaggtgat tccatgacct cacctgtagg
      481 tcaaagcatc cctgaaggtc atatggtgtt agtagatact ggacgggagc cagtgaatgg
      541 aagccttgtt gtagccaaac tgactgacgc gaacgaagca acattcaaga aactggtcat
      601 agatggcggt cagaagtacc tgaaaggcct gaatccttca tggcctatga ctcctatcaa
      661 cggaaactgc aagattatcg gtgttgtcgt ggaagcgagg gtaaaattcg tagctgcaaa
      721 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact
      781 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      841 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      901 ttctgcgttt atatactaga gttgacaaac aagatacatt gtatgatact acaagaaagt
      961 ttgttgatac tagagtcaca caggactact agatgaatac acaattgatg ggtgagcgta
     1021 ttcgcgctcg aagaaaaaaa ctcaagatta gacaagccgc tcttggtaag atggtgggag
     1081 tgtctaatgt tgcaatatcg caatgggagc gctcggagac tgagccaaat ggggagaacc
     1141 tgttggcact ttcgaaggct cttcagtgct cccctgacta tttgctgaaa ggagatttaa
     1201 gccagacaaa cgttgcctat catagtaggc atgagccaag aggatcatac cctcttatca
     1261 gttgggtaag cgcagggcaa tggatggaag ctgtagaacc ttatcacaag cgcgcgatag
     1321 agaactggca cgacaccact gtagattgtt cagaagattc attttggctt gatgtccaag
     1381 gtgactctat gacagcaccg gcagggttaa gcattccaga aggaatgata attctggttg
     1441 atcccgaagt cgaaccaaga aacggcaagc tggttgttgc aaaattagaa ggtgaaaacg
     1501 aggccacatt caaaaaatta gttatggatg caggccgaaa gtttttaaaa ccattaaacc
     1561 cacaatatcc gatgatagaa atcaacggaa actgcaaaat cattggcgta gttgttgacg
     1621 caaaactcgc aaatcttcca gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1681 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca
     1741 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1801 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//