##gff-version 3
##sequence-region BBa_K145259 1 1841
BBa_K145259	.	promoter	922	967	.	+	0	ID=BBa_R1052;Name=BBa_R1052
BBa_K145259	.	promoter	922	927	.	+	0	ID=annotation2082;Name=-35;Parent=BBa_R1052
BBa_K145259	.	promoter	923	928	.	+	0	ID=annotation2083;Name=-35;Parent=BBa_R1052
BBa_K145259	.	promoter	922	964	.	+	0	ID=annotation2079;Name=-10;Parent=BBa_R1052
BBa_K145259	.	start_codon	956	958	.	+	0	ID=annotation2084;Name=start;Parent=BBa_R1052
BBa_K145259	.	non_covalent_binding_site	929	942	.	+	0	ID=annotation2080;Name=OR2;Parent=BBa_R1052
BBa_K145259	.	engineered_region	922	967	.	+	0	ID=annotation7075;Name=BBa_R1052;Parent=BBa_R1052
BBa_K145259	.	promoter	945	950	.	+	0	ID=annotation2087;Name=-10;Parent=BBa_R1052
BBa_K145259	.	non_covalent_binding_site	951	964	.	+	0	ID=annotation2081;Name=OR1;Parent=BBa_R1052
BBa_K145259	.	engineered_region	976	1841	.	+	0	ID=BBa_P0353;Name=BBa_P0353
BBa_K145259	.	CDS	993	1679	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_P0353
BBa_K145259	.	stop_codon	1674	1679	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_K145259	.	engineered_tag	1680	1704	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_K145259	.	tag	1641	1673	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_K145259	.	CDS	993	1640	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_K145259	.	engineered_region	993	1679	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_K145259	.	terminator	1713	1792	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0353
BBa_K145259	.	stem_loop	1724	1767	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145259	.	engineered_region	1713	1792	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145259	.	ribosome_entry_site	976	986	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0353
BBa_K145259	.	engineered_region	976	986	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K145259	.	ribosome_entry_site	982	985	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K145259	.	ribosome_entry_site	976	986	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K145259	.	terminator	1801	1841	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0353
BBa_K145259	.	polyA_site	1828	1841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145259	.	stem_loop	1808	1827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145259	.	stop_codon	1834	1834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145259	.	engineered_region	1801	1841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145259	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_K145259	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_K145259	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_K145259	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_K145259	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_K145259	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_K145259	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_K145259	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_K145259	.	engineered_region	63	913	.	+	0	ID=BBa_P0152;Name=BBa_P0152
BBa_K145259	.	ribosome_entry_site	63	76	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0152
BBa_K145259	.	conserved_region	69	72	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K145259	.	CDS	83	751	.	+	0	ID=BBa_C0052;Name=BBa_C0052;Parent=BBa_P0152
BBa_K145259	.	engineered_tag	752	776	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_K145259	.	CDS	83	751	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_K145259	.	tag	713	751	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_K145259	.	engineered_region	83	751	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_K145259	.	terminator	785	864	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0152
BBa_K145259	.	stem_loop	796	839	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145259	.	engineered_region	785	864	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145259	.	terminator	873	913	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0152
BBa_K145259	.	polyA_site	900	913	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145259	.	stem_loop	880	899	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145259	.	stop_codon	906	906	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145259	.	engineered_region	873	913	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K145259
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaacctac
tagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtgg
ggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagc
tcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaa
gggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattg
atgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacc
tgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgta
gccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctga
atccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgt
agctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacaaacaagatacattgtatgata
ctacaagaaagtttgttgatactagagtcacacaggactactagatgaatacacaattgatgggtgagcgtattcgcgc
tcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgg
gagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgc
tgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttg
ggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgta
gattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaag
gaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacga
ggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaa
atcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaact
acgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttata