LOCUS BBa_K145265 4258 bp DNA linear 29-Nov-2024 DEFINITION test FILTER (old) ACCESSION BBa_K145265 VERSION BBa_K145265.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 63..156 /label=BBa_J23008 misc_feature 165..261 /label=BBa_J23009 stem_loop 281..324 /label=stem_loop misc_feature 270..349 /label=BBa_B0010 terminator 270..349 /label=BBa_B0010 stem_loop 365..384 /label=T7 TE misc_feature 391..391 /label=stop polyA_site 385..398 /label=polya misc_feature 358..398 /label=BBa_B0012 terminator 358..398 /label=BBa_B0012 misc_feature 1..398 /label=BBa_K145215 promoter 407..441 /label=BBa_J23109 misc_feature 450..492 /label=BBa_J23032 misc_feature 499..501 /label=start misc_feature 3148..3150 /label=stop misc_feature 3151..3153 /label=stop CDS 502..3147 /label=protein CDS 499..3153 /label=BBa_K145001 stem_loop 3173..3216 /label=stem_loop misc_feature 3162..3241 /label=BBa_B0010 terminator 3162..3241 /label=BBa_B0010 stem_loop 3257..3276 /label=T7 TE misc_feature 3283..3283 /label=stop polyA_site 3277..3290 /label=polya misc_feature 3250..3290 /label=BBa_B0012 terminator 3250..3290 /label=BBa_B0012 terminator 3162..3290 /label=BBa_B0015 misc_feature 407..3290 /label=BBa_K145278 misc_feature 1..3290 /label=BBa_K145275 promoter 3299..3344 /label=BBa_I712074 misc_feature 3353..3395 /label=BBa_J23032 CDS 3402..4121 /label=GFP protein CDS 3402..4121 /label=BBa_E0040 stem_loop 4141..4184 /label=stem_loop misc_feature 4130..4209 /label=BBa_B0010 terminator 4130..4209 /label=BBa_B0010 stem_loop 4225..4244 /label=T7 TE misc_feature 4251..4251 /label=stop polyA_site 4245..4258 /label=polya misc_feature 4218..4258 /label=BBa_B0012 terminator 4218..4258 /label=BBa_B0012 misc_feature 3299..4258 /label=BBa_K145272 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agacccaaaa gcaagaggtg attctagttg gtggttaatg aaaattaact tacttactag 121 aaatatctct aaaaagccag attattaatc cggctttact agagcgggcg ggcgggcggg 181 cgggatccat aaaaaaaaaa cccaaaagca agaggtgatt ctagttaaaa aaaaaaagct 241 tcccgcccgc ccgcccgccc gtactagagc caggcatcaa ataaaacgaa aggctcagtc 301 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 361 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttta cagctagctc 421 agtcctaggg actgtgctag ctactagaga actagaatca cctcttgctt ttgggtaaga 481 cagaagagga gatactagat gaacacgatt aacatcgcta agaacgactt ctctgacatc 541 gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc 601 gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg 661 tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc 721 actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct 781 aagcgcggca agcgcccgac agccttccag ttcctgcaag aaatcaagcc ggaagccgta 841 gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt 901 caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc 961 cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta 1021 gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt 1081 ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta 1141 cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct 1201 ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc 1261 gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct 1321 cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg 1381 gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct 1441 gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc 1501 ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg 1561 attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc 1621 accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc 1681 cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc 1741 tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg 1801 caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag 1861 gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg 1921 ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag 1981 tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc 2041 tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg 2101 gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta 2161 ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt 2221 gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta 2281 gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag 2341 gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc 2401 atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc 2461 attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct 2521 ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa 2581 gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag 2641 actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct 2701 gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag 2761 ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag 2821 gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact 2881 gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc 2941 ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac 3001 acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac 3061 gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac 3121 atcttagagt cggacttcgc gttcgcgtaa taatactaga gccaggcatc aaataaaacg 3181 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3241 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagta 3301 atacgactca ctatagggaa tacaagctac ttgttctttt tgcatactag agaactagaa 3361 tcacctcttg cttttgggta agacagaaga ggagatacta gatgcgtaaa ggagaagaac 3421 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat 3481 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta 3541 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg 3601 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg 3661 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca 3721 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag 3781 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact 3841 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa 3901 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc 3961 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc 4021 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg 4081 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa 4141 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 4201 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //