##gff-version 3
##sequence-region BBa_K145265 1 4258
BBa_K145265	.	engineered_region	3299	4258	.	+	0	ID=BBa_K145272;Name=BBa_K145272
BBa_K145265	.	mature_transcript_region	3353	3395	.	+	0	ID=BBa_J23032;Name=BBa_J23032;Parent=BBa_K145272
BBa_K145265	.	terminator	4218	4258	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145272
BBa_K145265	.	stop_codon	4251	4251	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145265	.	polyA_site	4245	4258	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145265	.	stem_loop	4225	4244	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145265	.	engineered_region	4218	4258	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145265	.	promoter	3299	3344	.	+	0	ID=BBa_I712074;Name=BBa_I712074;Parent=BBa_K145272
BBa_K145265	.	CDS	3402	4121	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K145272
BBa_K145265	.	CDS	3402	4121	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K145265	.	terminator	4130	4209	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145272
BBa_K145265	.	stem_loop	4141	4184	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145265	.	engineered_region	4130	4209	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145265	.	engineered_region	1	3290	.	+	0	ID=BBa_K145275;Name=BBa_K145275
BBa_K145265	.	engineered_region	1	398	.	+	0	ID=BBa_K145215;Name=BBa_K145215;Parent=BBa_K145275
BBa_K145265	.	mature_transcript_region	165	261	.	+	0	ID=BBa_J23009;Name=BBa_J23009;Parent=BBa_K145215
BBa_K145265	.	mature_transcript_region	63	156	.	+	0	ID=BBa_J23008;Name=BBa_J23008;Parent=BBa_K145215
BBa_K145265	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K145215
BBa_K145265	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K145265	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K145265	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K145265	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K145265	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K145265	.	terminator	358	398	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145215
BBa_K145265	.	stop_codon	391	391	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145265	.	polyA_site	385	398	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145265	.	stem_loop	365	384	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145265	.	engineered_region	358	398	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145265	.	terminator	270	349	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145215
BBa_K145265	.	stem_loop	281	324	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145265	.	engineered_region	270	349	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145265	.	engineered_region	407	3290	.	+	0	ID=BBa_K145278;Name=BBa_K145278;Parent=BBa_K145275
BBa_K145265	.	promoter	407	441	.	+	0	ID=BBa_J23109;Name=BBa_J23109;Parent=BBa_K145278
BBa_K145265	.	terminator	3162	3290	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145278
BBa_K145265	.	terminator	3162	3241	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145265	.	stem_loop	3173	3216	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145265	.	engineered_region	3162	3241	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145265	.	terminator	3250	3290	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145265	.	stop_codon	3283	3283	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145265	.	polyA_site	3277	3290	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145265	.	stem_loop	3257	3276	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145265	.	engineered_region	3250	3290	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145265	.	mature_transcript_region	450	492	.	+	0	ID=BBa_J23032;Name=BBa_J23032;Parent=BBa_K145278
BBa_K145265	.	CDS	499	3153	.	+	0	ID=BBa_K145001;Name=BBa_K145001;Parent=BBa_K145278
BBa_K145265	.	stop_codon	3148	3150	.	+	0	ID=annotation1968217;Name=stop;Parent=BBa_K145001
BBa_K145265	.	start_codon	499	501	.	+	0	ID=annotation1968216;Name=start;Parent=BBa_K145001
BBa_K145265	.	CDS	502	3147	.	+	0	ID=annotation1968243;Name=protein;Parent=BBa_K145001
BBa_K145265	.	stop_codon	3151	3153	.	+	0	ID=annotation1968218;Name=stop;Parent=BBa_K145001
>BBa_K145265
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggt
gattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggcttta
ctagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaa
gcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagtttacagctagctcagtcctagggactgtgctagctactagagaactagaatcacctcttgcttttgg
gtaagacagaagaggagatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgcta
tcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacga
gatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaag
cctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggca
agcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggc
ttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgc
ttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacg
tctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttc
gtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagctta
caccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaa
cccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattac
tggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctac
gaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcc
tagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactccc
gatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaag
gacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggcca
tctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgac
caaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaac
tgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcg
ctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgc
tggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcac
ttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacg
ggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgac
cgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggt
gttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgc
tggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacat
ggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgct
aagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactc
ctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccg
cttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaacttt
gtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcac
tgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacac
atatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatg
ccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactataggg
aatacaagctacttgttctttttgcatactagagaactagaatcacctcttgcttttgggtaagacagaagaggagata
ctagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcac
aaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaa
aactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatat
gaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggg
aactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatttta
aagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaaca
aaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaa
caaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaag
atcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaact
atacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata