BBa_K145265
type: ComponentDefinition
persistent identity: BBa K145265
display id: BBa_K145265
version: 1
was generated by: iGEM to SBOL conversion
description: test FILTER (old)
created: 2008-09-06T11:00:00Z
modified: 2015-05-08T01:10:29Z
owned by: myers
owned by: james
mutable description: Set up to test old filter
discontinued: false
dominant: false
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Nathalie Busschaert
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_K145272
component: BBa_K145275
sequence annotation: BBa_K145275
sequence annotation: BBa_K145272
sequence: https://synbiohub.org/public/igem/BBa_K145265_sequence/1

BBa_K145272
type: Component
persistent identity: component2235328
display id: component2235328
version: 1
top level: BBa_K145265
definition: BBa_K145272
access: public

BBa_K145275
type: Component
persistent identity: component2235317
display id: component2235317
version: 1
top level: BBa_K145265
definition: BBa_K145275
access: public

BBa_K145275
type: SequenceAnnotation
persistent identity: annotation2235317
display id: annotation2235317
version: 1
top level: BBa_K145265
location: https://synbiohub.org/public/igem/BBa_K145265/annotation2235317/range2235317/1
component: BBa_K145275

https://synbiohub.org/public/igem/BBa_K145265/annotation2235317/range2235317/1
type: Range
persistent identity: range2235317
display id: range2235317
version: 1
top level: BBa_K145265
direction: direction/reverse
start: 1
end: 3290
orientation: inline

BBa_K145272
type: SequenceAnnotation
persistent identity: annotation2235328
display id: annotation2235328
version: 1
top level: BBa_K145265
location: https://synbiohub.org/public/igem/BBa_K145265/annotation2235328/range2235328/1
component: BBa_K145272

https://synbiohub.org/public/igem/BBa_K145265/annotation2235328/range2235328/1
type: Range
persistent identity: range2235328
display id: range2235328
version: 1
top level: BBa_K145265
direction: direction/reverse
start: 3299
end: 4258
orientation: inline

BBa_K145275
type: ComponentDefinition
persistent identity: BBa K145275
display id: BBa_K145275
version: 1
was generated by: iGEM to SBOL conversion
description: T7 polymerase generator under TetR repressible promoter (filter)
created: 2008-09-06T11:00:00Z
modified: 2015-05-08T01:10:29Z
owned by: james
owned by: myers
mutable description: This device is part of a filter system. When there is no TetR the key will be generated. These keys will then open the lock and this will lead to the production of T7 DNA polymerase. When TetR is present, there will be no production of T7 DNA polymerase.
discontinued: false
dominant: false
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Nathalie Busschaert
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K145278
component: BBa_K145215
sequence annotation: BBa_K145215
sequence annotation: BBa_K145278
sequence: https://synbiohub.org/public/igem/BBa_K145275_sequence/1

BBa_K145278
type: Component
persistent identity: component2230171
display id: component2230171
version: 1
top level: BBa_K145275
definition: BBa_K145278
access: public

BBa_K145215
type: Component
persistent identity: component2230156
display id: component2230156
version: 1
top level: BBa_K145275
definition: BBa_K145215
access: public

BBa_K145215
type: SequenceAnnotation
persistent identity: annotation2230156
display id: annotation2230156
version: 1
top level: BBa_K145275
location: https://synbiohub.org/public/igem/BBa_K145275/annotation2230156/range2230156/1
component: BBa_K145215

https://synbiohub.org/public/igem/BBa_K145275/annotation2230156/range2230156/1
type: Range
persistent identity: range2230156
display id: range2230156
version: 1
top level: BBa_K145275
direction: direction/reverse
start: 1
end: 398
orientation: inline

BBa_K145278
type: SequenceAnnotation
persistent identity: annotation2230171
display id: annotation2230171
version: 1
top level: BBa_K145275
location: https://synbiohub.org/public/igem/BBa_K145275/annotation2230171/range2230171/1
component: BBa_K145278

https://synbiohub.org/public/igem/BBa_K145275/annotation2230171/range2230171/1
type: Range
persistent identity: range2230171
display id: range2230171
version: 1
top level: BBa_K145275
direction: direction/reverse
start: 407
end: 3290
orientation: inline

BBa_K145272
type: ComponentDefinition
persistent identity: BBa K145272
display id: BBa_K145272
version: 1
was generated by: iGEM to SBOL conversion
description: GFP regulated by AND-gate
created: 2008-09-06T11:00:00Z
modified: 2015-05-08T01:10:29Z
owned by: james
owned by: myers
mutable description: GFP will only be produced when both T7 DNA polymerase and ribokey3d are present. If only one or none of them are present, GFP will not be generated.
discontinued: false
dominant: false
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Nathalie Busschaert
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_I712074
component: BBa_E0040
component: BBa_J23032
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_I712074
sequence annotation: BBa_J23032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K145272_sequence/1

BBa_I712074
type: Component
persistent identity: component1975279
display id: component1975279
version: 1
top level: BBa_K145272
definition: BBa_I712074
access: public

BBa_E0040
type: Component
persistent identity: component1975282
display id: component1975282
version: 1
top level: BBa_K145272
definition: GFP
access: public

BBa_J23032
type: Component
persistent identity: component1975280
display id: component1975280
version: 1
top level: BBa_K145272
definition: BBa_J23032
access: public

BBa_B0012
type: Component
persistent identity: component1975285
display id: component1975285
version: 1
top level: BBa_K145272
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1975283
display id: component1975283
version: 1
top level: BBa_K145272
definition: BBa_B0010
access: public

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation1975279
display id: annotation1975279
version: 1
top level: BBa_K145272
location: https://synbiohub.org/public/igem/BBa_K145272/annotation1975279/range1975279/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K145272/annotation1975279/range1975279/1
type: Range
persistent identity: range1975279
display id: range1975279
version: 1
top level: BBa_K145272
direction: direction/reverse
start: 1
end: 46
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation1975280
display id: annotation1975280
version: 1
top level: BBa_K145272
location: https://synbiohub.org/public/igem/BBa_K145272/annotation1975280/range1975280/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K145272/annotation1975280/range1975280/1
type: Range
persistent identity: range1975280
display id: range1975280
version: 1
top level: BBa_K145272
direction: direction/reverse
start: 55
end: 97
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1975282
display id: annotation1975282
version: 1
top level: BBa_K145272
location: https://synbiohub.org/public/igem/BBa_K145272/annotation1975282/range1975282/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K145272/annotation1975282/range1975282/1
type: Range
persistent identity: range1975282
display id: range1975282
version: 1
top level: BBa_K145272
direction: direction/reverse
start: 104
end: 823
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1975283
display id: annotation1975283
version: 1
top level: BBa_K145272
location: https://synbiohub.org/public/igem/BBa_K145272/annotation1975283/range1975283/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K145272/annotation1975283/range1975283/1
type: Range
persistent identity: range1975283
display id: range1975283
version: 1
top level: BBa_K145272
direction: direction/forward
start: 832
end: 911
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1975285
display id: annotation1975285
version: 1
top level: BBa_K145272
location: https://synbiohub.org/public/igem/BBa_K145272/annotation1975285/range1975285/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K145272/annotation1975285/range1975285/1
type: Range
persistent identity: range1975285
display id: range1975285
version: 1
top level: BBa_K145272
direction: direction/forward
start: 920
end: 960
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (strong promoter from T7 bacteriophage)
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Rok Gaber
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

BBa_J23032
type: ComponentDefinition
persistent identity: BBa J23032
display id: BBa_J23032
version: 1
was generated by: iGEM to SBOL conversion
description: [lock3d]
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: Extension of overlapping oligonucleotides
owned by: james
owned by: myers
mutable description: lock3d
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J23032_sequence/1

BBa_K145215
type: ComponentDefinition
persistent identity: BBa K145215
display id: BBa_K145215
version: 1
was generated by: iGEM to SBOL conversion
description: FILTER Key (TetR promoter + key)
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:10:29Z
mutable provenance: other parts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: FILTER This device was designed to filter out noise in an input signal; in our case OmpF PoPs. If there is a short increase in transcription from this promoter, a small amount of J23066 RiboKey is produced. This is then used to unlock (J23078) the short lived UmuD-tagged T7 RNA polymerase. This results in an increase of active T7 protein. The final part of this filter is an AND gate which is used in the components further on in the system. This AND gate consists of a T7 RNA polymerase promoter with another RiboLock (J23078 again). The AND gate will only function if the duration of the noise is sufficiently long and intense (= a signal) in order to have both the short-lived RiboKey AND T7 polymerase available at the same time and thus to produce the component.
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We did a lot of modeling on this device. The most efficient Berkeley 2006 key and lock have been used. The RiboKy might be required in higher copy number although modeling doesn't predict this.
star: true
creator: Jonas Demeulemeester
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_J23009
component: BBa_J23008
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_J23008
sequence annotation: BBa_J23009
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K145215_sequence/1

BBa_B0012
type: Component
persistent identity: component1974071
display id: component1974071
version: 1
top level: BBa_K145215
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1974069
display id: component1974069
version: 1
top level: BBa_K145215
definition: BBa_B0010
access: public

BBa_J23009
type: Component
persistent identity: component1974068
display id: component1974068
version: 1
top level: BBa_K145215
definition: BBa_J23009
access: public

BBa_J23008
type: Component
persistent identity: component1974067
display id: component1974067
version: 1
top level: BBa_K145215
definition: BBa_J23008
access: public

BBa_R0040
type: Component
persistent identity: component1974063
display id: component1974063
version: 1
top level: BBa_K145215
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1974063
display id: annotation1974063
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974063/range1974063/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K145215/annotation1974063/range1974063/1
type: Range
persistent identity: range1974063
display id: range1974063
version: 1
top level: BBa_K145215
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_J23008
type: SequenceAnnotation
persistent identity: annotation1974067
display id: annotation1974067
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974067/range1974067/1
component: BBa_J23008

https://synbiohub.org/public/igem/BBa_K145215/annotation1974067/range1974067/1
type: Range
persistent identity: range1974067
display id: range1974067
version: 1
top level: BBa_K145215
direction: direction/reverse
start: 63
end: 156
orientation: inline

BBa_J23009
type: SequenceAnnotation
persistent identity: annotation1974068
display id: annotation1974068
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974068/range1974068/1
component: BBa_J23009

https://synbiohub.org/public/igem/BBa_K145215/annotation1974068/range1974068/1
type: Range
persistent identity: range1974068
display id: range1974068
version: 1
top level: BBa_K145215
direction: direction/reverse
start: 165
end: 261
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1974069
display id: annotation1974069
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974069/range1974069/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K145215/annotation1974069/range1974069/1
type: Range
persistent identity: range1974069
display id: range1974069
version: 1
top level: BBa_K145215
direction: direction/forward
start: 270
end: 349
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1974071
display id: annotation1974071
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974071/range1974071/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K145215/annotation1974071/range1974071/1
type: Range
persistent identity: range1974071
display id: range1974071
version: 1
top level: BBa_K145215
direction: direction/forward
start: 358
end: 398
orientation: inline

BBa_K145278
type: ComponentDefinition
persistent identity: BBa K145278
display id: BBa_K145278
version: 1
was generated by: iGEM to SBOL conversion
description: T7 DNA polymerase regulated by [lock3d]
created: 2008-09-06T11:00:00Z
modified: 2015-05-08T01:10:29Z
owned by: james
owned by: myers
mutable description: This part contains a T7 DNA polymerase under a constitutive promoter. The production of the polymerase is normally switched off because of the lock. It can only be switched on by the production of key3d.
discontinued: false
dominant: false
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Nathalie Busschaert
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23109
component: BBa_K145001
component: BBa_J23032
component: BBa_B0015
sequence annotation: BBa_J23109
sequence annotation: BBa_J23032
sequence annotation: BBa_K145001
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K145278_sequence/1

BBa_J23109
type: Component
persistent identity: component2222166
display id: component2222166
version: 1
top level: BBa_K145278
definition: BBa_J23109
access: public

BBa_K145001
type: Component
persistent identity: component2222172
display id: component2222172
version: 1
top level: BBa_K145278
definition: T7pol
access: public

BBa_J23032
type: Component
persistent identity: component2222167
display id: component2222167
version: 1
top level: BBa_K145278
definition: BBa_J23032
access: public

BBa_B0015
type: Component
persistent identity: component2222179
display id: component2222179
version: 1
top level: BBa_K145278
definition: BBa_B0015
access: public

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2222166
display id: annotation2222166
version: 1
top level: BBa_K145278
location: https://synbiohub.org/public/igem/BBa_K145278/annotation2222166/range2222166/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K145278/annotation2222166/range2222166/1
type: Range
persistent identity: range2222166
display id: range2222166
version: 1
top level: BBa_K145278
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation2222167
display id: annotation2222167
version: 1
top level: BBa_K145278
location: https://synbiohub.org/public/igem/BBa_K145278/annotation2222167/range2222167/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K145278/annotation2222167/range2222167/1
type: Range
persistent identity: range2222167
display id: range2222167
version: 1
top level: BBa_K145278
direction: direction/reverse
start: 44
end: 86
orientation: inline

BBa_K145001
type: SequenceAnnotation
persistent identity: annotation2222172
display id: annotation2222172
version: 1
top level: BBa_K145278
location: https://synbiohub.org/public/igem/BBa_K145278/annotation2222172/range2222172/1
component: BBa_K145001

https://synbiohub.org/public/igem/BBa_K145278/annotation2222172/range2222172/1
type: Range
persistent identity: range2222172
display id: range2222172
version: 1
top level: BBa_K145278
direction: direction/reverse
start: 93
end: 2747
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2222179
display id: annotation2222179
version: 1
top level: BBa_K145278
location: https://synbiohub.org/public/igem/BBa_K145278/annotation2222179/range2222179/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K145278/annotation2222179/range2222179/1
type: Range
persistent identity: range2222179
display id: range2222179
version: 1
top level: BBa_K145278
direction: direction/reverse
start: 2756
end: 2884
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_J23008
type: ComponentDefinition
persistent identity: BBa J23008
display id: BBa_J23008
version: 1
was generated by: iGEM to SBOL conversion
description: [key3c] riboregulator for lock3 variants
created: 2006-07-09T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: J23007
owned by: james
owned by: myers
mutable description: This part has the same homology region to J01122 as J23007 except that unlike J23007, J23008 does not contain the hairpin that was derived from J01129. J23008 anneals linearly to the stem of the hairpin in J01122 with perfect base pairing and destroys the hairpin in the process and reveals the RBS on J01122. When J01122 is transcribed the RNA secondary structure is such that the RBS hairpins with sequence upstream preventing the ribosome from binding and translation from occuring thereby locking up RFP further down stream. When J23008 is introduced into the cell in trans, we suspect that it will unlock the RBS for RFP as described above allowing translation to occur.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 931
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No design considerations
star: true
creator: Kaitlin Davis
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23008_sequence/1

BBa_J23009
type: ComponentDefinition
persistent identity: BBa J23009
display id: BBa_J23009
version: 1
was generated by: iGEM to SBOL conversion
description: [key3d] riboregulator for lock3 variants
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: Overlap extension
owned by: james
owned by: myers
mutable description: Variant of key3 (see part J01129 (key3), J23007(key3b), J23008(key3c)). Part is in pJ23006, so there is a Ptet upstream of the XbaI site to drive expression. Nevertheless, J23009 is still a basic part allowing both prefix and suffix insertions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23009_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

T7pol
type: ComponentDefinition
persistent identity: BBa K145001
display id: BBa_K145001
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA polymerase
created: 2008-07-07T11:00:00Z
modified: 2015-05-08T01:10:28Z
mutable provenance: BBa_I712022 LVA tag from BBa_E0022
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 RNA polymerase with LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Fuse the LVA tag to the T7 coding region
star: true
creator: Jonas Demeulemeester and Benjamien Moeyaert
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: protein
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1968216
display id: annotation1968216
version: 1
top level: T7pol
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968216/range1968216/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K145001/annotation1968216/range1968216/1
type: Range
persistent identity: range1968216
display id: range1968216
version: 1
top level: T7pol
direction: direction/forward
start: 1
end: 3
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation1968243
display id: annotation1968243
version: 1
top level: T7pol
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968243/range1968243/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K145001/annotation1968243/range1968243/1
type: Range
persistent identity: range1968243
display id: range1968243
version: 1
top level: T7pol
direction: direction/forward
start: 4
end: 2649
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968217
display id: annotation1968217
version: 1
top level: T7pol
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968217/range1968217/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K145001/annotation1968217/range1968217/1
type: Range
persistent identity: range1968217
display id: range1968217
version: 1
top level: T7pol
direction: direction/forward
start: 2650
end: 2652
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968218
display id: annotation1968218
version: 1
top level: T7pol
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968218/range1968218/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K145001/annotation1968218/range1968218/1
type: Range
persistent identity: range1968218
display id: range1968218
version: 1
top level: T7pol
direction: direction/forward
start: 2653
end: 2655
orientation: inline

BBa_J23109
type: ComponentDefinition
persistent identity: BBa J23109
display id: BBa_J23109
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23109_sequence/1

https://synbiohub.org/public/igem/BBa_K145265_sequence/1
type: Sequence
persistent identity: BBa K145265 sequence
display id: BBa_K145265_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctagggactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145275_sequence/1
type: Sequence
persistent identity: BBa K145275 sequence
display id: BBa_K145275_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctagggactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145272_sequence/1
type: Sequence
persistent identity: BBa K145272 sequence
display id: BBa_K145272_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712074_sequence/1
type: Sequence
persistent identity: BBa I712074 sequence
display id: BBa_I712074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23032_sequence/1
type: Sequence
persistent identity: BBa J23032 sequence
display id: BBa_J23032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145215_sequence/1
type: Sequence
persistent identity: BBa K145215 sequence
display id: BBa_K145215_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145278_sequence/1
type: Sequence
persistent identity: BBa K145278 sequence
display id: BBa_K145278_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23008_sequence/1
type: Sequence
persistent identity: BBa J23008 sequence
display id: BBa_J23008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23009_sequence/1
type: Sequence
persistent identity: BBa J23009 sequence
display id: BBa_J23009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145001_sequence/1
type: Sequence
persistent identity: BBa K145001 sequence
display id: BBa_K145001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23109_sequence/1
type: Sequence
persistent identity: BBa J23109 sequence
display id: BBa_J23109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z