LOCUS BBa_K145266 3764 bp DNA linear 22-Nov-2024 DEFINITION test hybrid promoter ACCESSION BBa_K145266 VERSION BBa_K145266.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23116 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..746 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 misc_feature 1..883 /label=BBa_K145201 misc_feature 892..910 /label=TetR 1 misc_feature 892..945 /label=BBa_R0040 promoter 911..916 /label=-35 misc_feature 917..935 /label=TetR 2 promoter 934..939 /label=-10 promoter 892..945 /label=BBa_R0040 RBS 954..964 /label=RBS-4\Weaker RBS 960..963 /label=RBS misc_feature 954..964 /label=BBa_B0033 RBS 954..964 /label=BBa_B0033 CDS 971..1618 /label=cII p22 misc_feature 1619..1651 /label=LVA misc_feature 1652..1657 /label=stop misc_feature 971..1657 /label=BBa_C0053 misc_feature 1658..1682 /label=Help:Barcodes CDS 971..1657 /label=BBa_C0053 stem_loop 1702..1745 /label=stem_loop misc_feature 1691..1770 /label=BBa_B0010 terminator 1691..1770 /label=BBa_B0010 stem_loop 1786..1805 /label=T7 TE misc_feature 1812..1812 /label=stop polyA_site 1806..1819 /label=polya misc_feature 1779..1819 /label=BBa_B0012 terminator 1779..1819 /label=BBa_B0012 misc_feature 954..1819 /label=BBa_P0353 misc_feature 892..1819 /label=BBa_K145273 misc_feature 1..1819 /label=BBa_K145268 promoter 1828..1862 /label=BBa_J23116 misc_feature 1875..1878 /label=conserved RBS 1871..1882 /label=BBa_B0034 misc_feature 1889..1890 /label=prefix misc_feature 2062..2062 /label=T misc_feature 2380..2380 /label=A CDS 1889..2638 /label=luxR misc_feature 1889..2644 /label=BBa_C0062 misc_feature 2645..2669 /label=Help:Barcodes CDS 1889..2644 /label=BBa_C0062 stem_loop 2689..2732 /label=stem_loop misc_feature 2678..2757 /label=BBa_B0010 terminator 2678..2757 /label=BBa_B0010 stem_loop 2773..2792 /label=T7 TE misc_feature 2799..2799 /label=stop polyA_site 2793..2806 /label=polya misc_feature 2766..2806 /label=BBa_B0012 terminator 2766..2806 /label=BBa_B0012 terminator 2678..2806 /label=BBa_B0015 misc_feature 1828..2806 /label=BBa_K145270 misc_feature 2815..2834 /label=Lux-box promoter 2834..2839 /label=-35 misc_feature 2837..2854 /label=OR2 promoter 2856..2861 /label=-10 misc_feature 2860..2877 /label=OR1 promoter 2815..2880 /label=BBa_K145150 RBS 2889..2901 /label=RBS-3\Weak RBS 2895..2898 /label=RBS misc_feature 2889..2901 /label=BBa_B0032 RBS 2889..2901 /label=BBa_B0032 CDS 2908..3627 /label=GFP protein CDS 2908..3627 /label=BBa_E0040 stem_loop 3647..3690 /label=stem_loop misc_feature 3636..3715 /label=BBa_B0010 terminator 3636..3715 /label=BBa_B0010 stem_loop 3731..3750 /label=T7 TE misc_feature 3757..3757 /label=stop polyA_site 3751..3764 /label=polya misc_feature 3724..3764 /label=BBa_B0012 terminator 3724..3764 /label=BBa_B0012 misc_feature 2815..3764 /label=BBa_K145269 misc_feature 1828..3764 /label=BBa_K145267 ORIGIN 1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagtcacaca 961 ggactactag atgaatacac aattgatggg tgagcgtatt cgcgctcgaa gaaaaaaact 1021 caagattaga caagccgctc ttggtaagat ggtgggagtg tctaatgttg caatatcgca 1081 atgggagcgc tcggagactg agccaaatgg ggagaacctg ttggcacttt cgaaggctct 1141 tcagtgctcc cctgactatt tgctgaaagg agatttaagc cagacaaacg ttgcctatca 1201 tagtaggcat gagccaagag gatcataccc tcttatcagt tgggtaagcg cagggcaatg 1261 gatggaagct gtagaacctt atcacaagcg cgcgatagag aactggcacg acaccactgt 1321 agattgttca gaagattcat tttggcttga tgtccaaggt gactctatga cagcaccggc 1381 agggttaagc attccagaag gaatgataat tctggttgat cccgaagtcg aaccaagaaa 1441 cggcaagctg gttgttgcaa aattagaagg tgaaaacgag gccacattca aaaaattagt 1501 tatggatgca ggccgaaagt ttttaaaacc attaaaccca caatatccga tgatagaaat 1561 caacggaaac tgcaaaatca ttggcgtagt tgttgacgca aaactcgcaa atcttccagc 1621 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc 1681 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 1741 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 1801 gggcctttct gcgtttatat actagagttg acagctagct cagtcctagg gactatgcta 1861 gctactagag aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag 1921 aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga 1981 tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat 2041 ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga 2101 tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc 2161 aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa 2221 agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa 2281 tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt 2341 tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa 2401 aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt 2461 agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga 2521 gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg 2581 ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta 2641 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag 2701 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 2761 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt 2821 aggatcgtac aggtttacta aagattcctt tagtttataa tttaagtgtt ctttaatttc 2881 tactagagtc acacaggaaa gtactagatg cgtaaaggag aagaactttt cactggagtt 2941 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga 3001 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga 3061 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt 3121 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt 3181 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa 3241 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa 3301 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac 3361 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt 3421 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc 3481 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc 3541 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat 3601 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc 3661 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 3721 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //