##gff-version 3
##sequence-region BBa_K145266 1 3764
BBa_K145266	.	engineered_region	1828	3764	.	+	0	ID=BBa_K145267;Name=BBa_K145267
BBa_K145266	.	engineered_region	2815	3764	.	+	0	ID=BBa_K145269;Name=BBa_K145269;Parent=BBa_K145267
BBa_K145266	.	terminator	3724	3764	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145269
BBa_K145266	.	stem_loop	3731	3750	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145266	.	stop_codon	3757	3757	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145266	.	polyA_site	3751	3764	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145266	.	engineered_region	3724	3764	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145266	.	CDS	2908	3627	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K145269
BBa_K145266	.	CDS	2908	3627	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K145266	.	promoter	2815	2880	.	+	0	ID=BBa_K145150;Name=BBa_K145150;Parent=BBa_K145269
BBa_K145266	.	promoter	2834	2839	.	+	0	ID=annotation1971583;Name=-35;Parent=BBa_K145150
BBa_K145266	.	non_covalent_binding_site	2815	2834	.	+	0	ID=annotation1971582;Name=Lux-box;Parent=BBa_K145150
BBa_K145266	.	non_covalent_binding_site	2837	2854	.	+	0	ID=annotation1971584;Name=OR2;Parent=BBa_K145150
BBa_K145266	.	non_covalent_binding_site	2860	2877	.	+	0	ID=annotation1971586;Name=OR1;Parent=BBa_K145150
BBa_K145266	.	promoter	2856	2861	.	+	0	ID=annotation1971585;Name=-10;Parent=BBa_K145150
BBa_K145266	.	terminator	3636	3715	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145269
BBa_K145266	.	engineered_region	3636	3715	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145266	.	stem_loop	3647	3690	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145266	.	ribosome_entry_site	2889	2901	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K145269
BBa_K145266	.	engineered_region	2889	2901	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145266	.	ribosome_entry_site	2895	2898	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145266	.	ribosome_entry_site	2889	2901	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145266	.	engineered_region	1828	2806	.	+	0	ID=BBa_K145270;Name=BBa_K145270;Parent=BBa_K145267
BBa_K145266	.	ribosome_entry_site	1871	1882	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145270
BBa_K145266	.	conserved_region	1875	1878	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K145266	.	CDS	1889	2644	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K145270
BBa_K145266	.	engineered_region	1889	1890	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K145266	.	engineered_region	1889	2644	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K145266	.	CDS	1889	2638	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K145266	.	point_mutation	2062	2062	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K145266	.	engineered_tag	2645	2669	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K145266	.	point_mutation	2380	2380	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K145266	.	promoter	1828	1862	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145270
BBa_K145266	.	terminator	2678	2806	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145270
BBa_K145266	.	terminator	2678	2757	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145266	.	engineered_region	2678	2757	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145266	.	stem_loop	2689	2732	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145266	.	terminator	2766	2806	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145266	.	stem_loop	2773	2792	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145266	.	stop_codon	2799	2799	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145266	.	polyA_site	2793	2806	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145266	.	engineered_region	2766	2806	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145266	.	engineered_region	1	1819	.	+	0	ID=BBa_K145268;Name=BBa_K145268
BBa_K145266	.	engineered_region	1	883	.	+	0	ID=BBa_K145201;Name=BBa_K145201;Parent=BBa_K145268
BBa_K145266	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201
BBa_K145266	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145266	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145266	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145266	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145266	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145266	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145266	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145266	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145266	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201
BBa_K145266	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K145266	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201
BBa_K145266	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K145266	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K145266	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K145266	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201
BBa_K145266	.	engineered_region	892	1819	.	+	0	ID=BBa_K145273;Name=BBa_K145273;Parent=BBa_K145268
BBa_K145266	.	engineered_region	954	1819	.	+	0	ID=BBa_P0353;Name=BBa_P0353;Parent=BBa_K145273
BBa_K145266	.	terminator	1691	1770	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0353
BBa_K145266	.	engineered_region	1691	1770	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145266	.	stem_loop	1702	1745	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145266	.	CDS	971	1657	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_P0353
BBa_K145266	.	engineered_tag	1658	1682	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_K145266	.	tag	1619	1651	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_K145266	.	stop_codon	1652	1657	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_K145266	.	engineered_region	971	1657	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_K145266	.	CDS	971	1618	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_K145266	.	ribosome_entry_site	954	964	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0353
BBa_K145266	.	ribosome_entry_site	954	964	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K145266	.	engineered_region	954	964	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K145266	.	ribosome_entry_site	960	963	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K145266	.	terminator	1779	1819	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0353
BBa_K145266	.	stem_loop	1786	1805	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145266	.	stop_codon	1812	1812	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145266	.	polyA_site	1806	1819	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145266	.	engineered_region	1779	1819	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145266	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K145273
BBa_K145266	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K145266	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K145266	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K145266	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K145266	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K145266
ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagtcacacaggactactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagatt
agacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaa
atggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagac
aaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatg
gaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcatttt
ggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcc
cgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagtt
atggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatca
ttggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataaca
ctgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgaaaaa
cataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgctta
tctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgata
tttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagt
agattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaat
gtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaa
tgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaat
tgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaa
aaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactg
tcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaac
aggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttactaaaga
ttcctttagtttataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatgcgtaaaggagaag
aacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggcca
acacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttca
agagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctga
agtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattctt
ggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagtta
acttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcga
tggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagac
cacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata