LOCUS BBa_K145267 1937 bp DNA linear 22-Nov-2024 DEFINITION luxR generator + GFP under control of luxR ACCESSION BBa_K145267 VERSION BBa_K145267.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23116 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 62..63 /label=prefix misc_feature 235..235 /label=T misc_feature 553..553 /label=A CDS 62..811 /label=luxR misc_feature 62..817 /label=BBa_C0062 misc_feature 818..842 /label=Help:Barcodes CDS 62..817 /label=BBa_C0062 stem_loop 862..905 /label=stem_loop misc_feature 851..930 /label=BBa_B0010 terminator 851..930 /label=BBa_B0010 stem_loop 946..965 /label=T7 TE misc_feature 972..972 /label=stop polyA_site 966..979 /label=polya misc_feature 939..979 /label=BBa_B0012 terminator 939..979 /label=BBa_B0012 terminator 851..979 /label=BBa_B0015 misc_feature 1..979 /label=BBa_K145270 misc_feature 988..1007 /label=Lux-box promoter 1007..1012 /label=-35 misc_feature 1010..1027 /label=OR2 promoter 1029..1034 /label=-10 misc_feature 1033..1050 /label=OR1 promoter 988..1053 /label=BBa_K145150 RBS 1062..1074 /label=RBS-3\Weak RBS 1068..1071 /label=RBS misc_feature 1062..1074 /label=BBa_B0032 RBS 1062..1074 /label=BBa_B0032 CDS 1081..1800 /label=GFP protein CDS 1081..1800 /label=BBa_E0040 stem_loop 1820..1863 /label=stem_loop misc_feature 1809..1888 /label=BBa_B0010 terminator 1809..1888 /label=BBa_B0010 stem_loop 1904..1923 /label=T7 TE misc_feature 1930..1930 /label=stop polyA_site 1924..1937 /label=polya misc_feature 1897..1937 /label=BBa_B0012 terminator 1897..1937 /label=BBa_B0012 misc_feature 988..1937 /label=BBa_K145269 ORIGIN 1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta 61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac 421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca 481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt 541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa 601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc 661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa 721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac 781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc 841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta ctaaagattc 1021 ctttagttta taatttaagt gttctttaat ttctactaga gtcacacagg aaagtactag 1081 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 1141 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga 1201 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt 1261 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag 1321 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc 1381 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt 1441 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa 1501 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga 1561 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac 1621 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 1681 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt 1741 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa 1801 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1861 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1921 gcctttctgc gtttata //