##gff-version 3
##sequence-region BBa_K145267 1 1937
BBa_K145267	.	engineered_region	1	979	.	+	0	ID=BBa_K145270;Name=BBa_K145270
BBa_K145267	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145270
BBa_K145267	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K145267	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K145270
BBa_K145267	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K145267	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K145267	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K145267	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K145267	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K145267	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K145267	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145270
BBa_K145267	.	terminator	851	979	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145270
BBa_K145267	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145267	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145267	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145267	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145267	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145267	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145267	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145267	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145267	.	engineered_region	988	1937	.	+	0	ID=BBa_K145269;Name=BBa_K145269
BBa_K145267	.	CDS	1081	1800	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K145269
BBa_K145267	.	CDS	1081	1800	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K145267	.	promoter	988	1053	.	+	0	ID=BBa_K145150;Name=BBa_K145150;Parent=BBa_K145269
BBa_K145267	.	non_covalent_binding_site	1033	1050	.	+	0	ID=annotation1971586;Name=OR1;Parent=BBa_K145150
BBa_K145267	.	promoter	1007	1012	.	+	0	ID=annotation1971583;Name=-35;Parent=BBa_K145150
BBa_K145267	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation1971582;Name=Lux-box;Parent=BBa_K145150
BBa_K145267	.	promoter	1029	1034	.	+	0	ID=annotation1971585;Name=-10;Parent=BBa_K145150
BBa_K145267	.	non_covalent_binding_site	1010	1027	.	+	0	ID=annotation1971584;Name=OR2;Parent=BBa_K145150
BBa_K145267	.	terminator	1809	1888	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145269
BBa_K145267	.	stem_loop	1820	1863	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145267	.	engineered_region	1809	1888	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145267	.	ribosome_entry_site	1062	1074	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K145269
BBa_K145267	.	engineered_region	1062	1074	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145267	.	ribosome_entry_site	1068	1071	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145267	.	ribosome_entry_site	1062	1074	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145267	.	terminator	1897	1937	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145269
BBa_K145267	.	stop_codon	1930	1930	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145267	.	polyA_site	1924	1937	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145267	.	stem_loop	1904	1923	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145267	.	engineered_region	1897	1937	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K145267
ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttactaaagattcctttagt
ttataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcac
tggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggt
gatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtca
ctactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccat
gcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagttt
gaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaat
tggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaat
tagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtc
cttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcc
ttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata