##gff-version 3
##sequence-region BBa_K145268 1 1819
BBa_K145268	.	engineered_region	1	883	.	+	0	ID=BBa_K145201;Name=BBa_K145201
BBa_K145268	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201
BBa_K145268	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K145268	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201
BBa_K145268	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145268	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145268	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145268	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145268	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145268	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145268	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145268	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145268	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201
BBa_K145268	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K145268	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K145268	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K145268	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201
BBa_K145268	.	engineered_region	892	1819	.	+	0	ID=BBa_K145273;Name=BBa_K145273
BBa_K145268	.	engineered_region	954	1819	.	+	0	ID=BBa_P0353;Name=BBa_P0353;Parent=BBa_K145273
BBa_K145268	.	terminator	1691	1770	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0353
BBa_K145268	.	engineered_region	1691	1770	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145268	.	stem_loop	1702	1745	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145268	.	CDS	971	1657	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_P0353
BBa_K145268	.	engineered_region	971	1657	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_K145268	.	tag	1619	1651	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_K145268	.	CDS	971	1618	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_K145268	.	stop_codon	1652	1657	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_K145268	.	engineered_tag	1658	1682	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_K145268	.	terminator	1779	1819	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0353
BBa_K145268	.	engineered_region	1779	1819	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145268	.	polyA_site	1806	1819	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145268	.	stop_codon	1812	1812	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145268	.	stem_loop	1786	1805	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145268	.	ribosome_entry_site	954	964	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0353
BBa_K145268	.	ribosome_entry_site	960	963	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K145268	.	engineered_region	954	964	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K145268	.	ribosome_entry_site	954	964	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K145268	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K145273
BBa_K145268	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K145268	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K145268	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K145268	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K145268	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
>BBa_K145268
ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagtcacacaggactactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagatt
agacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaa
atggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagac
aaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatg
gaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcatttt
ggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcc
cgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagtt
atggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatca
ttggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataaca
ctgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
ta