LOCUS       BBa_K145271              969 bp    DNA     linear       25-Nov-2024
DEFINITION  GFP regulated by AND-gate
ACCESSION   BBa_K145271
VERSION     BBa_K145271.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..106
                     /label=BBa_J23078
     CDS             113..832
                     /label=GFP protein
     CDS             113..832
                     /label=BBa_E0040
     stem_loop       852..895
                     /label=stem_loop
     misc_feature    841..920
                     /label=BBa_B0010
     terminator      841..920
                     /label=BBa_B0010
     stem_loop       936..955
                     /label=T7 TE
     misc_feature    962..962
                     /label=stop
     polyA_site      956..969
                     /label=polya
     misc_feature    929..969
                     /label=BBa_B0012
     terminator      929..969
                     /label=BBa_B0012
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtgtaga
       61 ccgaactaga atcacctctt gcttttgggt aagacagaag aggagatact agatgcgtaa
      121 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      181 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      241 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      301 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
      361 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      421 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
      481 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
      541 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
      601 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
      661 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
      721 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
      781 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag
      841 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      901 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      961 gcgtttata
//