LOCUS       BBa_K145273              928 bp    DNA     linear       22-Dec-2024
DEFINITION  c2 P22 under TetR repressible promoter
ACCESSION   BBa_K145273
VERSION     BBa_K145273.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..73
                     /label=RBS-4\Weaker
     RBS             69..72
                     /label=RBS
     misc_feature    63..73
                     /label=BBa_B0033
     RBS             63..73
                     /label=BBa_B0033
     CDS             80..727
                     /label=cII p22
     misc_feature    728..760
                     /label=LVA
     misc_feature    761..766
                     /label=stop
     misc_feature    80..766
                     /label=BBa_C0053
     misc_feature    767..791
                     /label=Help:Barcodes
     CDS             80..766
                     /label=BBa_C0053
     stem_loop       811..854
                     /label=stem_loop
     misc_feature    800..879
                     /label=BBa_B0010
     terminator      800..879
                     /label=BBa_B0010
     stem_loop       895..914
                     /label=T7 TE
     misc_feature    921..921
                     /label=stop
     polyA_site      915..928
                     /label=polya
     misc_feature    888..928
                     /label=BBa_B0012
     terminator      888..928
                     /label=BBa_B0012
     misc_feature    63..928
                     /label=BBa_P0353
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gactactaga tgaatacaca attgatgggt gagcgtattc gcgctcgaag
      121 aaaaaaactc aagattagac aagccgctct tggtaagatg gtgggagtgt ctaatgttgc
      181 aatatcgcaa tgggagcgct cggagactga gccaaatggg gagaacctgt tggcactttc
      241 gaaggctctt cagtgctccc ctgactattt gctgaaagga gatttaagcc agacaaacgt
      301 tgcctatcat agtaggcatg agccaagagg atcataccct cttatcagtt gggtaagcgc
      361 agggcaatgg atggaagctg tagaacctta tcacaagcgc gcgatagaga actggcacga
      421 caccactgta gattgttcag aagattcatt ttggcttgat gtccaaggtg actctatgac
      481 agcaccggca gggttaagca ttccagaagg aatgataatt ctggttgatc ccgaagtcga
      541 accaagaaac ggcaagctgg ttgttgcaaa attagaaggt gaaaacgagg ccacattcaa
      601 aaaattagtt atggatgcag gccgaaagtt tttaaaacca ttaaacccac aatatccgat
      661 gatagaaatc aacggaaact gcaaaatcat tggcgtagtt gttgacgcaa aactcgcaaa
      721 tcttccagct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
      781 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      841 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      901 ccttcgggtg ggcctttctg cgtttata
//