##gff-version 3
##sequence-region BBa_K145273 1 928
BBa_K145273	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K145273	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K145273	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K145273	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K145273	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K145273	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K145273	.	engineered_region	63	928	.	+	0	ID=BBa_P0353;Name=BBa_P0353
BBa_K145273	.	CDS	80	766	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_P0353
BBa_K145273	.	tag	728	760	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_K145273	.	engineered_tag	767	791	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_K145273	.	stop_codon	761	766	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_K145273	.	CDS	80	727	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_K145273	.	engineered_region	80	766	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_K145273	.	terminator	888	928	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0353
BBa_K145273	.	engineered_region	888	928	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145273	.	stem_loop	895	914	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145273	.	stop_codon	921	921	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145273	.	polyA_site	915	928	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145273	.	ribosome_entry_site	63	73	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0353
BBa_K145273	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K145273	.	ribosome_entry_site	63	73	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K145273	.	engineered_region	63	73	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K145273	.	terminator	800	879	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0353
BBa_K145273	.	engineered_region	800	879	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145273	.	stem_loop	811	854	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K145273
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggactactag
atgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaaga
tggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcact
ttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtagg
catgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcaca
agcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactc
tatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggc
aagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttt
taaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaa
actcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatca
ctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata