LOCUS BBa_K145276 3299 bp DNA linear 05-Dec-2025 DEFINITION T7 polymerase generator under TetR repressible promoter ACCESSION BBa_K145276 VERSION BBa_K145276.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 63..156 /label=BBa_J23008 misc_feature 165..261 /label=BBa_J23009 stem_loop 281..324 /label=stem_loop misc_feature 270..349 /label=BBa_B0010 terminator 270..349 /label=BBa_B0010 stem_loop 365..384 /label=T7 TE misc_feature 391..391 /label=stop polyA_site 385..398 /label=polya misc_feature 358..398 /label=BBa_B0012 terminator 358..398 /label=BBa_B0012 misc_feature 1..398 /label=BBa_K145215 promoter 407..441 /label=BBa_J23109 misc_feature 450..501 /label=BBa_J23078 misc_feature 508..510 /label=start misc_feature 3157..3159 /label=stop misc_feature 3160..3162 /label=stop CDS 511..3156 /label=protein CDS 508..3162 /label=BBa_K145001 stem_loop 3182..3225 /label=stem_loop misc_feature 3171..3250 /label=BBa_B0010 terminator 3171..3250 /label=BBa_B0010 stem_loop 3266..3285 /label=T7 TE misc_feature 3292..3292 /label=stop polyA_site 3286..3299 /label=polya misc_feature 3259..3299 /label=BBa_B0012 terminator 3259..3299 /label=BBa_B0012 terminator 3171..3299 /label=BBa_B0015 misc_feature 407..3299 /label=BBa_K145277 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agacccaaaa gcaagaggtg attctagttg gtggttaatg aaaattaact tacttactag 121 aaatatctct aaaaagccag attattaatc cggctttact agagcgggcg ggcgggcggg 181 cgggatccat aaaaaaaaaa cccaaaagca agaggtgatt ctagttaaaa aaaaaaagct 241 tcccgcccgc ccgcccgccc gtactagagc caggcatcaa ataaaacgaa aggctcagtc 301 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 361 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttta cagctagctc 421 agtcctaggg actgtgctag ctactagagt gtagaccgaa ctagaatcac ctcttgcttt 481 tgggtaagac agaagaggag atactagatg aacacgatta acatcgctaa gaacgacttc 541 tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt 601 ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc 661 cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag 721 cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa 781 gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg 841 gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat 901 acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc 961 ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac 1021 aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc 1081 tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat 1141 gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc 1201 caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct 1261 gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc 1321 gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt 1381 cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt 1441 tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac 1501 aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac 1561 atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct 1621 gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc 1681 aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat 1741 aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg 1801 ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca 1861 atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat 1921 aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct 1981 tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc 2041 cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc 2101 cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga 2161 gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac 2221 gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat 2281 aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg 2341 ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag 2401 cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg 2461 gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat 2521 caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct 2581 gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa 2641 gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat 2701 ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc 2761 ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca 2821 cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt 2881 cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac 2941 gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact 3001 atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac 3061 cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac 3121 ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaat aatactagag ccaggcatca 3181 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 3241 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //