##gff-version 3
##sequence-region BBa_K145276 1 3299
BBa_K145276	.	engineered_region	1	398	.	+	0	ID=BBa_K145215;Name=BBa_K145215
BBa_K145276	.	terminator	358	398	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145215
BBa_K145276	.	stop_codon	391	391	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145276	.	polyA_site	385	398	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145276	.	stem_loop	365	384	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145276	.	engineered_region	358	398	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145276	.	mature_transcript_region	165	261	.	+	0	ID=BBa_J23009;Name=BBa_J23009;Parent=BBa_K145215
BBa_K145276	.	mature_transcript_region	63	156	.	+	0	ID=BBa_J23008;Name=BBa_J23008;Parent=BBa_K145215
BBa_K145276	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K145215
BBa_K145276	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K145276	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K145276	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K145276	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K145276	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K145276	.	terminator	270	349	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145215
BBa_K145276	.	engineered_region	270	349	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145276	.	stem_loop	281	324	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145276	.	engineered_region	407	3299	.	+	0	ID=BBa_K145277;Name=BBa_K145277
BBa_K145276	.	CDS	508	3162	.	+	0	ID=BBa_K145001;Name=BBa_K145001;Parent=BBa_K145277
BBa_K145276	.	start_codon	508	510	.	+	0	ID=annotation1968216;Name=start;Parent=BBa_K145001
BBa_K145276	.	CDS	511	3156	.	+	0	ID=annotation1968243;Name=protein;Parent=BBa_K145001
BBa_K145276	.	stop_codon	3157	3159	.	+	0	ID=annotation1968217;Name=stop;Parent=BBa_K145001
BBa_K145276	.	stop_codon	3160	3162	.	+	0	ID=annotation1968218;Name=stop;Parent=BBa_K145001
BBa_K145276	.	mature_transcript_region	450	501	.	+	0	ID=BBa_J23078;Name=BBa_J23078;Parent=BBa_K145277
BBa_K145276	.	promoter	407	441	.	+	0	ID=BBa_J23109;Name=BBa_J23109;Parent=BBa_K145277
BBa_K145276	.	terminator	3171	3299	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145277
BBa_K145276	.	terminator	3171	3250	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145276	.	engineered_region	3171	3250	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145276	.	stem_loop	3182	3225	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145276	.	terminator	3259	3299	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145276	.	stop_codon	3292	3292	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145276	.	polyA_site	3286	3299	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145276	.	stem_loop	3266	3285	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145276	.	engineered_region	3259	3299	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K145276
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggt
gattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggcttta
ctagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaa
gcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagtttacagctagctcagtcctagggactgtgctagctactagagtgtagaccgaactagaatcacctct
tgcttttgggtaagacagaagaggagatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaac
tggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatga
gtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgct
gccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagcta
agcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagac
cactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggac
gaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcg
tagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggc
gtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatg
gttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgagg
ctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggac
tggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactg
atgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaaca
agaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtga
agaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtg
taccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaacc
ataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaa
cgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccac
ggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatca
tggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctt
tgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggc
atccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcagg
acatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagt
taccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctg
gcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtc
aacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgc
tggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaag
tctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcatt
gggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcgg
tcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgct
cctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaat
cttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactat
ggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattg
gacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata