LOCUS       BBa_K145277             2893 bp    DNA     linear       22-Dec-2024
DEFINITION  T7 DNA polymerase regulated by lock
ACCESSION   BBa_K145277
VERSION     BBa_K145277.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    44..95
                     /label=BBa_J23078
     misc_feature    102..104
                     /label=start
     misc_feature    2751..2753
                     /label=stop
     misc_feature    2754..2756
                     /label=stop
     CDS             105..2750
                     /label=protein
     CDS             102..2756
                     /label=BBa_K145001
     stem_loop       2776..2819
                     /label=stem_loop
     misc_feature    2765..2844
                     /label=BBa_B0010
     terminator      2765..2844
                     /label=BBa_B0010
     stem_loop       2860..2879
                     /label=T7 TE
     misc_feature    2886..2886
                     /label=stop
     polyA_site      2880..2893
                     /label=polya
     misc_feature    2853..2893
                     /label=BBa_B0012
     terminator      2853..2893
                     /label=BBa_B0012
     terminator      2765..2893
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagtgtagac cgaactagaa
       61 tcacctcttg cttttgggta agacagaaga ggagatacta gatgaacacg attaacatcg
      121 ctaagaacga cttctctgac atcgaactgg ctgctatccc gttcaacact ctggctgacc
      181 attacggtga gcgtttagct cgcgaacagt tggcccttga gcatgagtct tacgagatgg
      241 gtgaagcacg cttccgcaag atgtttgagc gtcaacttaa agctggtgag gttgcggata
      301 acgctgccgc caagcctctc atcactaccc tactccctaa gatgattgca cgcatcaacg
      361 actggtttga ggaagtgaaa gctaagcgcg gcaagcgccc gacagccttc cagttcctgc
      421 aagaaatcaa gccggaagcc gtagcgtaca tcaccattaa gaccactctg gcttgcctaa
      481 ccagtgctga caatacaacc gttcaggctg tagcaagcgc aatcggtcgg gccattgagg
      541 acgaggctcg cttcggtcgt atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg
      601 aggaacaact caacaagcgc gtagggcacg tctacaagaa agcatttatg caagttgtcg
      661 aggctgacat gctctctaag ggtctactcg gtggcgaggc gtggtcttcg tggcataagg
      721 aagactctat tcatgtagga gtacgctgca tcgagatgct cattgagtca accggaatgg
      781 ttagcttaca ccgccaaaat gctggcgtag taggtcaaga ctctgagact atcgaactcg
      841 cacctgaata cgctgaggct atcgcaaccc gtgcaggtgc gctggctggc atctctccga
      901 tgttccaacc ttgcgtagtt cctcctaagc cgtggactgg cattactggt ggtggctatt
      961 gggctaacgg tcgtcgtcct ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc
     1021 gctacgaaga cgtttacatg cctgaggtgt acaaagcgat taacattgcg caaaacaccg
     1081 catggaaaat caacaagaaa gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt
     1141 gtccggtcga ggacatccct gcgattgagc gtgaagaact cccgatgaaa ccggaagaca
     1201 tcgacatgaa tcctgaggct ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca
     1261 aggacaaggc tcgcaagtct cgccgtatca gccttgagtt catgcttgag caagccaata
     1321 agtttgctaa ccataaggcc atctggttcc cttacaacat ggactggcgc ggtcgtgttt
     1381 acgctgtgtc aatgttcaac ccgcaaggta acgatatgac caaaggactg cttacgctgg
     1441 cgaaaggtaa accaatcggt aaggaaggtt actactggct gaaaatccac ggtgcaaact
     1501 gtgcgggtgt cgataaggtt ccgttccctg agcgcatcaa gttcattgag gaaaaccacg
     1561 agaacatcat ggcttgcgct aagtctccac tggagaacac ttggtgggct gagcaagatt
     1621 ctccgttctg cttccttgcg ttctgctttg agtacgctgg ggtacagcac cacggcctga
     1681 gctataactg ctcccttccg ctggcgtttg acgggtcttg ctctggcatc cagcacttct
     1741 ccgcgatgct ccgagatgag gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg
     1801 ttcaggacat ctacgggatt gttgctaaga aagtcaacga gattctacaa gcagacgcaa
     1861 tcaatgggac cgataacgaa gtagttaccg tgaccgatga gaacactggt gaaatctctg
     1921 agaaagtcaa gctgggcact aaggcactgg ctggtcaatg gctggcttac ggtgttactc
     1981 gcagtgtgac taagcgttca gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc
     2041 gtcaacaagt gctggaagat accattcagc cagctattga ttccggcaag ggtctgatgt
     2101 tcactcagcc gaatcaggct gctggataca tggctaagct gatttgggaa tctgtgagcg
     2161 tgacggtggt agctgcggtt gaagcaatga actggcttaa gtctgctgct aagctgctgg
     2221 ctgctgaggt caaagataag aagactggag agattcttcg caagcgttgc gctgtgcatt
     2281 gggtaactcc tgatggtttc cctgtgtggc aggaatacaa gaagcctatt cagacgcgct
     2341 tgaacctgat gttcctcggt cagttccgct tacagcctac cattaacacc aacaaagata
     2401 gcgagattga tgcacacaaa caggagtctg gtatcgctcc taactttgta cacagccaag
     2461 acggtagcca ccttcgtaag actgtagtgt gggcacacga gaagtacgga atcgaatctt
     2521 ttgcactgat tcacgactcc ttcggtacca ttccggctga cgctgcgaac ctgttcaaag
     2581 cagtgcgcga aactatggtt gacacatatg agtcttgtga tgtactggct gatttctacg
     2641 accagttcgc tgaccagttg cacgagtctc aattggacaa aatgccagca cttccggcta
     2701 aaggtaactt gaacctccgt gacatcttag agtcggactt cgcgttcgcg taataatact
     2761 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     2821 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2881 ttctgcgttt ata
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