LOCUS       BBa_K145278             2884 bp    DNA     linear       17-May-2024
DEFINITION  T7 DNA polymerase regulated by [lock3d]
ACCESSION   BBa_K145278
VERSION     BBa_K145278.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    44..86
                     /label=BBa_J23032
     misc_feature    93..95
                     /label=start
     misc_feature    2742..2744
                     /label=stop
     misc_feature    2745..2747
                     /label=stop
     CDS             96..2741
                     /label=protein
     CDS             93..2747
                     /label=BBa_K145001
     stem_loop       2767..2810
                     /label=stem_loop
     misc_feature    2756..2835
                     /label=BBa_B0010
     terminator      2756..2835
                     /label=BBa_B0010
     stem_loop       2851..2870
                     /label=T7 TE
     misc_feature    2877..2877
                     /label=stop
     polyA_site      2871..2884
                     /label=polya
     misc_feature    2844..2884
                     /label=BBa_B0012
     terminator      2844..2884
                     /label=BBa_B0012
     terminator      2756..2884
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaactaga atcacctctt
       61 gcttttgggt aagacagaag aggagatact agatgaacac gattaacatc gctaagaacg
      121 acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac cattacggtg
      181 agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg ggtgaagcac
      241 gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat aacgctgccg
      301 ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac gactggtttg
      361 aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg caagaaatca
      421 agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta accagtgctg
      481 acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag gacgaggctc
      541 gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac
      601 tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc gaggctgaca
      661 tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag gaagactcta
      721 ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg gttagcttac
      781 accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc gcacctgaat
      841 acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg atgttccaac
      901 cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat tgggctaacg
      961 gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg cgctacgaag
     1021 acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa
     1081 tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg
     1141 aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac atcgacatga
     1201 atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg
     1261 ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat aagtttgcta
     1321 accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt
     1381 caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg gcgaaaggta
     1441 aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg
     1501 tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac gagaacatca
     1561 tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat tctccgttct
     1621 gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg agctataact
     1681 gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc tccgcgatgc
     1741 tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca
     1801 tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga
     1861 ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca
     1921 agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga
     1981 ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag
     2041 tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc
     2101 cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg
     2161 tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg
     2221 tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc
     2281 ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttgaacctga
     2341 tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat agcgagattg
     2401 atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa gacggtagcc
     2461 accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct tttgcactga
     2521 ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg
     2581 aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac gaccagttcg
     2641 ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct aaaggtaact
     2701 tgaacctccg tgacatctta gagtcggact tcgcgttcgc gtaataatac tagagccagg
     2761 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2821 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2881 tata
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