BBa_K145280
type: ComponentDefinition
persistent identity: BBa K145280
display id: BBa_K145280
version: 1
was generated by: iGEM to SBOL conversion
description: GFP-LVA under TetR control
created: 2008-09-06T11:00:00Z
modified: 2015-05-08T01:10:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This device consists of a TetR generator and a GFP with LVA tag under control of a TetR promoter. Normally the production of GFP-LVA is switched off. It can be switched on by the addition of tetracyclin.
discontinued: false
dominant: false
experience: experience/Works
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Nathalie Busschaert
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K145205
component: BBa_K145201
sequence annotation: BBa_K145201
sequence annotation: BBa_K145205
sequence: https://synbiohub.org/public/igem/BBa_K145280_sequence/1

BBa_K145205
type: Component
persistent identity: component2282064
display id: component2282064
version: 1
top level: BBa_K145280
definition: BBa_K145205
access: public

BBa_K145201
type: Component
persistent identity: component2282043
display id: component2282043
version: 1
top level: BBa_K145280
definition: BBa_K145201
access: public

BBa_K145201
type: SequenceAnnotation
persistent identity: annotation2282043
display id: annotation2282043
version: 1
top level: BBa_K145280
location: https://synbiohub.org/public/igem/BBa_K145280/annotation2282043/range2282043/1
component: BBa_K145201

https://synbiohub.org/public/igem/BBa_K145280/annotation2282043/range2282043/1
type: Range
persistent identity: range2282043
display id: range2282043
version: 1
top level: BBa_K145280
direction: direction/reverse
start: 1
end: 883
orientation: inline

BBa_K145205
type: SequenceAnnotation
persistent identity: annotation2282064
display id: annotation2282064
version: 1
top level: BBa_K145280
location: https://synbiohub.org/public/igem/BBa_K145280/annotation2282064/range2282064/1
component: BBa_K145205

https://synbiohub.org/public/igem/BBa_K145280/annotation2282064/range2282064/1
type: Range
persistent identity: range2282064
display id: range2282064
version: 1
top level: BBa_K145280
direction: direction/reverse
start: 892
end: 1868
orientation: inline

BBa_K145201
type: ComponentDefinition
persistent identity: BBa K145201
display id: BBa_K145201
version: 1
was generated by: iGEM to SBOL conversion
description: INPUT TetR generator
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:10:29Z
mutable provenance: other parts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: INPUT TetR constitutive generator
discontinued: false
dominant: false
experience: experience/Works
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: chosen a medium-weak constitutive promoter. In order to have strong repression but also easy derepression by aTc.
star: true
creator: Jonas Demeulemeester
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_B0034
component: BBa_J23116
component: BBa_B0015
sequence annotation: BBa_J23116
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K145201_sequence/1

BBa_C0040
type: Component
persistent identity: component2254374
display id: component2254374
version: 1
top level: BBa_K145201
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component2254370
display id: component2254370
version: 1
top level: BBa_K145201
definition: BBa_B0034
access: public

BBa_J23116
type: Component
persistent identity: component2254368
display id: component2254368
version: 1
top level: BBa_K145201
definition: BBa_J23116
access: public

BBa_B0015
type: Component
persistent identity: component2254381
display id: component2254381
version: 1
top level: BBa_K145201
definition: BBa_B0015
access: public

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2254368
display id: annotation2254368
version: 1
top level: BBa_K145201
location: https://synbiohub.org/public/igem/BBa_K145201/annotation2254368/range2254368/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K145201/annotation2254368/range2254368/1
type: Range
persistent identity: range2254368
display id: range2254368
version: 1
top level: BBa_K145201
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2254370
display id: annotation2254370
version: 1
top level: BBa_K145201
location: https://synbiohub.org/public/igem/BBa_K145201/annotation2254370/range2254370/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K145201/annotation2254370/range2254370/1
type: Range
persistent identity: range2254370
display id: range2254370
version: 1
top level: BBa_K145201
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2254374
display id: annotation2254374
version: 1
top level: BBa_K145201
location: https://synbiohub.org/public/igem/BBa_K145201/annotation2254374/range2254374/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K145201/annotation2254374/range2254374/1
type: Range
persistent identity: range2254374
display id: range2254374
version: 1
top level: BBa_K145201
direction: direction/reverse
start: 62
end: 746
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2254381
display id: annotation2254381
version: 1
top level: BBa_K145201
location: https://synbiohub.org/public/igem/BBa_K145201/annotation2254381/range2254381/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K145201/annotation2254381/range2254381/1
type: Range
persistent identity: range2254381
display id: range2254381
version: 1
top level: BBa_K145201
direction: direction/reverse
start: 755
end: 883
orientation: inline

BBa_K145205
type: ComponentDefinition
persistent identity: BBa K145205
display id: BBa_K145205
version: 1
was generated by: iGEM to SBOL conversion
description: OUTPUT GFP under TetR pomoter
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:10:29Z
mutable provenance: other parts
owned by: james
owned by: myers
mutable description: OUTPUT GFP with an LVA tag under the control of the OmpF pomoter, which is activated by a low concentration and repressed by a high concentration of phosphorylated OmpR.
discontinued: false
dominant: false
experience: experience/Works
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: When light hits the lightsensor M30109 (K145000). It's kinase activity will be inhibited and only a small amount of OmpR-P is produced. This means the OmpF promoter will be actively used for transcription.
star: true
creator: Jonas Demeulemeester
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K145015
component: BBa_B0032
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_K145015
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K145205_sequence/1

BBa_K145015
type: Component
persistent identity: component1973997
display id: component1973997
version: 1
top level: BBa_K145205
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component1973990
display id: component1973990
version: 1
top level: BBa_K145205
definition: BBa_B0032
access: public

BBa_R0040
type: Component
persistent identity: component1973985
display id: component1973985
version: 1
top level: BBa_K145205
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1974000
display id: component1974000
version: 1
top level: BBa_K145205
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1973998
display id: component1973998
version: 1
top level: BBa_K145205
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1973985
display id: annotation1973985
version: 1
top level: BBa_K145205
location: https://synbiohub.org/public/igem/BBa_K145205/annotation1973985/range1973985/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K145205/annotation1973985/range1973985/1
type: Range
persistent identity: range1973985
display id: range1973985
version: 1
top level: BBa_K145205
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1973990
display id: annotation1973990
version: 1
top level: BBa_K145205
location: https://synbiohub.org/public/igem/BBa_K145205/annotation1973990/range1973990/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K145205/annotation1973990/range1973990/1
type: Range
persistent identity: range1973990
display id: range1973990
version: 1
top level: BBa_K145205
direction: direction/forward
start: 63
end: 75
orientation: inline

BBa_K145015
type: SequenceAnnotation
persistent identity: annotation1973997
display id: annotation1973997
version: 1
top level: BBa_K145205
location: https://synbiohub.org/public/igem/BBa_K145205/annotation1973997/range1973997/1
component: BBa_K145015

https://synbiohub.org/public/igem/BBa_K145205/annotation1973997/range1973997/1
type: Range
persistent identity: range1973997
display id: range1973997
version: 1
top level: BBa_K145205
direction: direction/reverse
start: 82
end: 840
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1973998
display id: annotation1973998
version: 1
top level: BBa_K145205
location: https://synbiohub.org/public/igem/BBa_K145205/annotation1973998/range1973998/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K145205/annotation1973998/range1973998/1
type: Range
persistent identity: range1973998
display id: range1973998
version: 1
top level: BBa_K145205
direction: direction/forward
start: 849
end: 928
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1974000
display id: annotation1974000
version: 1
top level: BBa_K145205
location: https://synbiohub.org/public/igem/BBa_K145205/annotation1974000/range1974000/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K145205/annotation1974000/range1974000/1
type: Range
persistent identity: range1974000
display id: range1974000
version: 1
top level: BBa_K145205
direction: direction/forward
start: 937
end: 977
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

GFP
type: ComponentDefinition
persistent identity: BBa K145015
display id: BBa_K145015
version: 1
was generated by: iGEM to SBOL conversion
description: GFP with LVA tag
created: 2008-07-31T11:00:00Z
modified: 2015-05-08T01:10:28Z
mutable provenance: pJBA111, carrying GFP-LVA, was generously provided by Dr. S. Molin, The Technical University of Denmark, Lyngby Appl Environ Microbiol. 1998 Jun;64(6):2240-6. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S. PMID: 9603842
owned by: james
owned by: myers
mutable description: Green Fluorescent Protein with LVA tag for rapid degradation
discontinued: false
dominant: false
experience: experience/Works
group u list: _257_
m user id: 0
owner id: 2939
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Designed out of pJBA111 (Molin, 1998) with PCR to add appropriote prefix and suffix.
star: true
creator: Benjamien Moeyaert
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: start
sequence annotation: cds
sequence annotation: LVA tag
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145015_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1969616
display id: annotation1969616
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_K145015/annotation1969616/range1969616/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K145015/annotation1969616/range1969616/1
type: Range
persistent identity: range1969616
display id: range1969616
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1969620
display id: annotation1969620
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_K145015/annotation1969620/range1969620/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K145015/annotation1969620/range1969620/1
type: Range
persistent identity: range1969620
display id: range1969620
version: 1
top level: GFP
direction: direction/forward
start: 4
end: 716
orientation: inline

LVA tag
type: SequenceAnnotation
persistent identity: annotation1969619
display id: annotation1969619
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_K145015/annotation1969619/range1969619/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K145015/annotation1969619/range1969619/1
type: Range
persistent identity: range1969619
display id: range1969619
version: 1
top level: GFP
direction: direction/forward
start: 717
end: 753
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1969617
display id: annotation1969617
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_K145015/annotation1969617/range1969617/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K145015/annotation1969617/range1969617/1
type: Range
persistent identity: range1969617
display id: range1969617
version: 1
top level: GFP
direction: direction/forward
start: 754
end: 756
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1969618
display id: annotation1969618
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_K145015/annotation1969618/range1969618/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K145015/annotation1969618/range1969618/1
type: Range
persistent identity: range1969618
display id: range1969618
version: 1
top level: GFP
direction: direction/forward
start: 757
end: 759
orientation: inline

https://synbiohub.org/public/igem/BBa_K145280_sequence/1
type: Sequence
persistent identity: BBa K145280 sequence
display id: BBa_K145280_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145201_sequence/1
type: Sequence
persistent identity: BBa K145201 sequence
display id: BBa_K145201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145205_sequence/1
type: Sequence
persistent identity: BBa K145205 sequence
display id: BBa_K145205_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23116_sequence/1
type: Sequence
persistent identity: BBa J23116 sequence
display id: BBa_J23116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145015_sequence/1
type: Sequence
persistent identity: BBa K145015 sequence
display id: BBa_K145015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z