LOCUS       BBa_K145301             1382 bp    DNA     linear       22-Dec-2024
DEFINITION  lacI controlled by key/lock
ACCESSION   BBa_K145301
VERSION     BBa_K145301.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    44..86
                     /label=BBa_J23032
     misc_feature    1182..1220
                     /label=LVA
     CDS             93..1220
                     /label=lacI-LVA
     misc_feature    93..1220
                     /label=BBa_C0012
     misc_feature    1221..1245
                     /label=Help:Barcodes
     CDS             93..1220
                     /label=BBa_C0012
     stem_loop       1265..1308
                     /label=stem_loop
     misc_feature    1254..1333
                     /label=BBa_B0010
     terminator      1254..1333
                     /label=BBa_B0010
     stem_loop       1349..1368
                     /label=T7 TE
     misc_feature    1375..1375
                     /label=stop
     polyA_site      1369..1382
                     /label=polya
     misc_feature    1342..1382
                     /label=BBa_B0012
     terminator      1342..1382
                     /label=BBa_B0012
     terminator      1254..1382
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaactaga atcacctctt
       61 gcttttgggt aagacagaag aggagatact agatggtgaa tgtgaaacca gtaacgttat
      121 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
      181 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
      241 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
      301 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
      361 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
      421 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
      481 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
      541 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
      601 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
      661 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
      721 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
      781 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
      841 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
      901 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa
      961 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     1021 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     1081 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1141 tgcagctggc acgacaggtt tcccgactgg aaagcgggca ggctgcaaac gacgaaaact
     1201 acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca
     1261 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1321 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1381 ta
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