LOCUS       BBa_K145302             2252 bp    DNA     linear       26-Dec-2024
DEFINITION  luxI generator controlled by key/lock
ACCESSION   BBa_K145302
VERSION     BBa_K145302.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    44..86
                     /label=BBa_J23032
     misc_feature    1182..1220
                     /label=LVA
     CDS             93..1220
                     /label=lacI-LVA
     misc_feature    93..1220
                     /label=BBa_C0012
     misc_feature    1221..1245
                     /label=Help:Barcodes
     CDS             93..1220
                     /label=BBa_C0012
     stem_loop       1265..1308
                     /label=stem_loop
     misc_feature    1254..1333
                     /label=BBa_B0010
     terminator      1254..1333
                     /label=BBa_B0010
     stem_loop       1349..1368
                     /label=T7 TE
     misc_feature    1375..1375
                     /label=stop
     polyA_site      1369..1382
                     /label=polya
     misc_feature    1342..1382
                     /label=BBa_B0012
     terminator      1342..1382
                     /label=BBa_B0012
     terminator      1254..1382
                     /label=BBa_B0015
     misc_feature    1..1382
                     /label=BBa_K145301
     misc_feature    1393..1409
                     /label=lac O1
     promoter        1410..1415
                     /label=-35
     misc_feature    1416..1432
                     /label=lac O1
     promoter        1433..1438
                     /label=-10
     misc_feature    1391..1444
                     /label=BBa_R0011
     promoter        1391..1444
                     /label=BBa_R0011
     RBS             1454..1466
                     /label=RBS-3\Weak
     RBS             1460..1463
                     /label=RBS
     misc_feature    1454..1466
                     /label=BBa_B0032
     RBS             1454..1466
                     /label=BBa_B0032
     misc_feature    2052..2083
                     /label=LVA
     CDS             1473..2051
                     /label=luxI
     misc_feature    1473..2090
                     /label=BBa_C0061
     misc_feature    2091..2115
                     /label=Help:Barcodes
     CDS             1473..2090
                     /label=BBa_C0061
     stem_loop       2135..2178
                     /label=stem_loop
     misc_feature    2124..2203
                     /label=BBa_B0010
     terminator      2124..2203
                     /label=BBa_B0010
     stem_loop       2219..2238
                     /label=T7 TE
     misc_feature    2245..2245
                     /label=stop
     polyA_site      2239..2252
                     /label=polya
     misc_feature    2212..2252
                     /label=BBa_B0012
     terminator      2212..2252
                     /label=BBa_B0012
     terminator      2124..2252
                     /label=BBa_B0015
     misc_feature    1391..2252
                     /label=BBa_K145254
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaactaga atcacctctt
       61 gcttttgggt aagacagaag aggagatact agatggtgaa tgtgaaacca gtaacgttat
      121 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
      181 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
      241 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
      301 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
      361 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
      421 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
      481 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
      541 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
      601 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
      661 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
      721 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
      781 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
      841 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
      901 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa
      961 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     1021 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     1081 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1141 tgcagctggc acgacaggtt tcccgactgg aaagcgggca ggctgcaaac gacgaaaact
     1201 acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca
     1261 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1321 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1381 tatactagag aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga
     1441 gcacatacta gagtcacaca ggaaagtact agatgactat aatgataaaa aaatcggatt
     1501 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta
     1561 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata
     1621 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc
     1681 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc
     1741 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa
     1801 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat
     1861 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag
     1921 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg
     1981 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag
     2041 cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
     2101 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2161 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2221 ctcaccttcg ggtgggcctt tctgcgttta ta
//