##gff-version 3
##sequence-region BBa_K145302 1 2252
BBa_K145302	.	engineered_region	1391	2252	.	+	0	ID=BBa_K145254;Name=BBa_K145254
BBa_K145302	.	promoter	1391	1444	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K145254
BBa_K145302	.	promoter	1410	1415	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K145302	.	promoter	1433	1438	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K145302	.	operator	1393	1409	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K145302	.	operator	1416	1432	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K145302	.	engineered_region	1391	1444	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K145302	.	ribosome_entry_site	1454	1466	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K145254
BBa_K145302	.	ribosome_entry_site	1460	1463	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145302	.	engineered_region	1454	1466	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145302	.	ribosome_entry_site	1454	1466	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145302	.	terminator	2124	2252	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145254
BBa_K145302	.	terminator	2124	2203	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145302	.	stem_loop	2135	2178	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145302	.	engineered_region	2124	2203	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145302	.	terminator	2212	2252	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145302	.	engineered_region	2212	2252	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145302	.	stop_codon	2245	2245	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145302	.	stem_loop	2219	2238	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145302	.	polyA_site	2239	2252	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145302	.	CDS	1473	2090	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K145254
BBa_K145302	.	engineered_region	1473	2090	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K145302	.	tag	2052	2083	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K145302	.	engineered_tag	2091	2115	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K145302	.	CDS	1473	2051	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K145302	.	engineered_region	1	1382	.	+	0	ID=BBa_K145301;Name=BBa_K145301
BBa_K145302	.	terminator	1254	1382	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145301
BBa_K145302	.	terminator	1254	1333	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145302	.	stem_loop	1265	1308	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145302	.	engineered_region	1254	1333	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145302	.	terminator	1342	1382	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145302	.	engineered_region	1342	1382	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145302	.	stop_codon	1375	1375	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145302	.	stem_loop	1349	1368	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145302	.	polyA_site	1369	1382	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145302	.	CDS	93	1220	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_K145301
BBa_K145302	.	engineered_tag	1221	1245	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K145302	.	CDS	93	1220	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K145302	.	tag	1182	1220	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K145302	.	engineered_region	93	1220	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K145302	.	mature_transcript_region	44	86	.	+	0	ID=BBa_J23032;Name=BBa_J23032;Parent=BBa_K145301
BBa_K145302	.	promoter	1	35	.	+	0	ID=BBa_J23109;Name=BBa_J23109;Parent=BBa_K145301
>BBa_K145302
tttacagctagctcagtcctagggactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaa
gaggagatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagacc
gtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctga
attacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggc
cctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatg
gtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcatta
actatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctc
tgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattg
ggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaat
atctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccat
gcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgcc
attaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttata
tcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctca
gggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaa
accgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctg
caaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgag
cggataacaagatactgagcacatactagagtcacacaggaaagtactagatgactataatgataaaaaaatcggattt
tttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggac
ttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactg
aaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttgg
tcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaat
aactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatg
taacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaat
tcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgct
gcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata