LOCUS       BBa_K1455002            2402 bp    DNA     linear       25-Jan-2026
DEFINITION  pBAD - luxO D47E - GFP
ACCESSION   BBa_K1455002
VERSION     BBa_K1455002.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..159
                     /label=Start
     misc_feature    1516..1518
                     /label=Stop
     misc_feature    295..297
                     /label=Mutation
     CDS             157..1518
                     /label=BBa_K218015
     RBS             1527..1539
                     /label=RBS-3\Weak
     RBS             1533..1536
                     /label=RBS
     misc_feature    1527..1539
                     /label=BBa_B0032
     RBS             1527..1539
                     /label=BBa_B0032
     CDS             1546..2265
                     /label=GFP protein
     CDS             1546..2265
                     /label=BBa_E0040
     stem_loop       2285..2328
                     /label=stem_loop
     misc_feature    2274..2353
                     /label=BBa_B0010
     terminator      2274..2353
                     /label=BBa_B0010
     stem_loop       2369..2388
                     /label=T7 TE
     misc_feature    2395..2395
                     /label=stop
     polyA_site      2389..2402
                     /label=polya
     misc_feature    2362..2402
                     /label=BBa_B0012
     terminator      2362..2402
                     /label=BBa_B0012
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg tagaagacac cgcatccgtt
      181 gcggcacttt accgctctta cctcacgcca cttggcatcg atatcaatat tgttggaaca
      241 ggcagagacg ccattgaaag cctgaaccat cgcattcctg atcttattct gctcgagctc
      301 cgtctacctg atatgacggg gatggacgta ttgcacgcgg tgaagaaaag ccacccagac
      361 gtgccaatca tcttcatgac agcccatggt tctatcgata ctgcggtaga ggcgatgcgc
      421 cacggttctc aagacttcct aatcaaacca tgtgaagcag accgtttacg tgtcacggtg
      481 aacaatgcga tccgtaaagc aaccaaatta aagaatgaag ctgacaaccc cggtaaccaa
      541 aattaccaag gcttcatcgg cagtagccaa acgatgcagc aggtttaccg caccattgac
      601 tcggcagcga gcagtaaagc gagtattttc atcacgggtg aaagtggtac gggtaaagaa
      661 gtgtgtgccg aagcgattca cgcagcaagc aaacgcggtg ataagccgtt tatcgccatc
      721 aactgtgcgg caatcccgaa agaccttatt gaaagtgagc tgtttggtca cgtaaaaggt
      781 gcgtttactg gtgctgcgaa tgaccgacaa ggtgcggcag agcttgctga tggcggcacc
      841 ttgttccttg atgaactctg tgaaatggac ttggatcttc aaactaagct attgcgcttt
      901 atccaaacgg gtacattcca aaaagtcggt tcttctaaaa tgaagagcgt ggatgtgcgc
      961 tttgtgtgtg caactaaccg agacccttgg aaagaagtgc aagaaggccg tttccgtgaa
     1021 gacttgtatt accgtttgta cgtgattcct ttgcaccttc cgccgctgcg tgagcgtggt
     1081 aaagacgtta ttgaaattgc atactcgttg cttggttata tgtctcatga ggaaggtaag
     1141 agtttcgtcc gtttcgcaca agacgtgatt gaaagattca acagctacga atggccgggt
     1201 aacgttcgcc agttgcaaaa cgtattgcgt aatatcgtgg tactgaacaa tggcaaagag
     1261 atcacgctgg atatgttacc gccaccactg aatcagcctg ttgtgcgcca atcggtagca
     1321 aaattcattg aacctgacat tatgacggtg tcagatatta tgccgctttg gatgacagag
     1381 aaaatggcta ttgagcaggc aattcaagcg tgtgaaggca acattccacg cgctgctggc
     1441 tatttggatg ttagtccatc aacgatttat cgcaagttgc aagcttggaa tagcaaggac
     1501 gaaaaacaaa acgtatgata ctagagtcac acaggaaagt actagatgcg taaaggagaa
     1561 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     1621 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     1681 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
     1741 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
     1801 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     1861 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
     1921 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
     1981 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
     2041 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     2101 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     2161 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     2221 gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca
     2281 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2341 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2401 ta
//