LOCUS       BBa_K1455004            1597 bp    DNA     linear       15-Nov-2024
DEFINITION  QPI (TetR inverter) - AmilCP
ACCESSION   BBa_K1455004
VERSION     BBa_K1455004.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..678
                     /label=BBa_C0040
     stem_loop       723..766
                     /label=stem_loop
     misc_feature    712..791
                     /label=BBa_B0010
     terminator      712..791
                     /label=BBa_B0010
     stem_loop       807..826
                     /label=T7 TE
     misc_feature    833..833
                     /label=stop
     polyA_site      827..840
                     /label=polya
     misc_feature    800..840
                     /label=BBa_B0012
     terminator      800..840
                     /label=BBa_B0012
     misc_feature    849..867
                     /label=TetR 1
     misc_feature    849..902
                     /label=BBa_R0040
     promoter        868..873
                     /label=-35
     misc_feature    874..892
                     /label=TetR 2
     promoter        891..896
                     /label=-10
     promoter        849..902
                     /label=BBa_R0040
     misc_feature    1..902
                     /label=BBa_Q04400
     misc_feature    915..918
                     /label=conserved
     RBS             911..922
                     /label=BBa_B0034
     CDS             929..1594
                     /label=amilCP
     CDS             929..1597
                     /label=BBa_K592009
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      901 actactagag aaagaggaga aatactagat gagtgtgatc gctaaacaaa tgacctacaa
      961 ggtttatatg tcaggcacgg tcaatggaca ctactttgag gtcgaaggcg atggaaaagg
     1021 taagccctac gagggggagc agacggtaaa gctcactgtc accaagggcg gacctctgcc
     1081 atttgcttgg gatattttat caccacagtg tcagtacgga agcataccat tcaccaagta
     1141 ccctgaagac atccctgact atgtaaagca gtcattcccg gagggctata catgggagag
     1201 gatcatgaac tttgaagatg gtgcagtgtg tactgtcagc aatgattcca gcatccaagg
     1261 caactgtttc atctaccatg tcaagttctc tggtttgaac tttcctccca atggacctgt
     1321 catgcagaag aagacacagg gctgggaacc caacactgag cgtctctttg cacgagatgg
     1381 aatgctgcta ggaaacaact ttatggctct gaagttagaa ggaggcggtc actatttgtg
     1441 tgaatttaaa actacttaca aggcaaagaa gcctgtgaag atgccagggt atcactatgt
     1501 tgaccgcaaa ctggatgtaa ccaatcacaa caaggattac acttcggttg agcagtgtga
     1561 aatttccatt gcacgcaaac ctgtggtcgc ctaataa
//