LOCUS       BBa_K1455005             994 bp    DNA     linear       18-May-2024
DEFINITION  pTet - AmilCP - pag activator (PSP3)
ACCESSION   BBa_K1455005
VERSION     BBa_K1455005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..746
                     /label=amilCP
     CDS             81..749
                     /label=BBa_K592009
     misc_feature    758..769
                     /label=B0034
     misc_feature    776..776
                     /label=PSP3 pag
     CDS             776..994
                     /label=PSP3 pag
     misc_feature    758..994
                     /label=BBa_I746351
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgagtgtga tcgctaaaca aatgacctac aaggtttata
      121 tgtcaggcac ggtcaatgga cactactttg aggtcgaagg cgatggaaaa ggtaagccct
      181 acgaggggga gcagacggta aagctcactg tcaccaaggg cggacctctg ccatttgctt
      241 gggatatttt atcaccacag tgtcagtacg gaagcatacc attcaccaag taccctgaag
      301 acatccctga ctatgtaaag cagtcattcc cggagggcta tacatgggag aggatcatga
      361 actttgaaga tggtgcagtg tgtactgtca gcaatgattc cagcatccaa ggcaactgtt
      421 tcatctacca tgtcaagttc tctggtttga actttcctcc caatggacct gtcatgcaga
      481 agaagacaca gggctgggaa cccaacactg agcgtctctt tgcacgagat ggaatgctgc
      541 taggaaacaa ctttatggct ctgaagttag aaggaggcgg tcactatttg tgtgaattta
      601 aaactactta caaggcaaag aagcctgtga agatgccagg gtatcactat gttgaccgca
      661 aactggatgt aaccaatcac aacaaggatt acacttcggt tgagcagtgt gaaatttcca
      721 ttgcacgcaa acctgtggtc gcctaataat actagagaaa gaggagaaat actagatgat
      781 gcactgcccg ttatgccaaa acgctgcaca tgctcgcact agccggtacc ttagcaccga
      841 aacgaaagaa cgttatcacc agtgccaaaa cataaattgc ggatgtacat ttatcacttt
      901 tgagacacta tcaagattca ttgtgaaacc ggggactgtt gatcctgctc cgccccaccc
      961 catcagaaac caacaacagc aactttggct ttga
//