LOCUS BBa_K1455006 1095 bp DNA linear 16-Dec-2024 DEFINITION psid - pTet - AmilCP - pag activator (PSP3) Positive feedback ACCESSION BBa_K1455006 VERSION BBa_K1455006.1 FEATURES Location/Qualifiers promoter 1..93 /label=Psid promoter 1..93 /label=BBa_I746364 misc_feature 102..120 /label=TetR 1 misc_feature 102..155 /label=BBa_R0040 promoter 121..126 /label=-35 misc_feature 127..145 /label=TetR 2 promoter 144..149 /label=-10 promoter 102..155 /label=BBa_R0040 misc_feature 168..171 /label=conserved RBS 164..175 /label=BBa_B0034 CDS 182..847 /label=amilCP CDS 182..850 /label=BBa_K592009 misc_feature 859..870 /label=B0034 misc_feature 877..877 /label=PSP3 pag CDS 877..1095 /label=PSP3 pag misc_feature 859..1095 /label=BBa_I746351 misc_feature 102..1095 /label=BBa_K1455005 ORIGIN 1 tggcttgccg gtctgaggat gagtctcctg tgtcagggct ggcacatctg caatgcgtcg 61 tgttgttgtc cggtgtacgt cacaattttc ttatactaga gtccctatca gtgatagaga 121 ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta 181 gatgagtgtg atcgctaaac aaatgaccta caaggtttat atgtcaggca cggtcaatgg 241 acactacttt gaggtcgaag gcgatggaaa aggtaagccc tacgaggggg agcagacggt 301 aaagctcact gtcaccaagg gcggacctct gccatttgct tgggatattt tatcaccaca 361 gtgtcagtac ggaagcatac cattcaccaa gtaccctgaa gacatccctg actatgtaaa 421 gcagtcattc ccggagggct atacatggga gaggatcatg aactttgaag atggtgcagt 481 gtgtactgtc agcaatgatt ccagcatcca aggcaactgt ttcatctacc atgtcaagtt 541 ctctggtttg aactttcctc ccaatggacc tgtcatgcag aagaagacac agggctggga 601 acccaacact gagcgtctct ttgcacgaga tggaatgctg ctaggaaaca actttatggc 661 tctgaagtta gaaggaggcg gtcactattt gtgtgaattt aaaactactt acaaggcaaa 721 gaagcctgtg aagatgccag ggtatcacta tgttgaccgc aaactggatg taaccaatca 781 caacaaggat tacacttcgg ttgagcagtg tgaaatttcc attgcacgca aacctgtggt 841 cgcctaataa tactagagaa agaggagaaa tactagatga tgcactgccc gttatgccaa 901 aacgctgcac atgctcgcac tagccggtac cttagcaccg aaacgaaaga acgttatcac 961 cagtgccaaa acataaattg cggatgtaca tttatcactt ttgagacact atcaagattc 1021 attgtgaaac cggggactgt tgatcctgct ccgccccacc ccatcagaaa ccaacaacag 1081 caactttggc tttga //