BBa_K1459002 1 BBa_K1459002 C-term of PmrB from Salmonella enterica 2014-09-30T11:00:00Z 2015-05-08T01:10:32Z We obtained PmrA-PmrB thanks to kindness of prof. He from the University of Chicago. PmrB is a transmembrane kinase. After binding iron (III) ion by binding peptide on extracellular loop, it's intracellular domain gains kinase activity and phosphorylates PmrA (BBa_K1459000). PmrB C-term is a part of two-component system. When fused with some binding tag, PmrB(N-term), PmrA and pmrC-reporter, it is a viable detecting system. false false _1837_ 0 20803 9 It's complicated false We had to use Silver (RFC 23) Standard instead of RFC 10 to allow protein fusions. false Grzegorz Ścibisz BBa_K1459002_sequence 1 gaggcggtcgccagcctgatcgtccccagcgtatttatggttagcctgacgctgctggtttgctaccaggcggtacggcgtattacccgcccactcgccgaactgcaaaaagagctggaagcgcggacggcggataatctgacgccgatcgccattcacagctccacgcttgagattgagtccgtcgtctcggcgatcaatcaactggttacgcgtttgaccaccacgctcgacaatgaacgcctttttaccgccgatgtggcccatgagctacgcacgccgctgtcgggggtgcgtttgcatctggaattattgtcaaaaacccacaatgttgatgtcgcgccgcttatcgcccgtcttgaccagatgatggatagcgtctcccagcttctgcaactggcgcgcgtgggccagtcattctcttccgggaattatcaggaagtaaaactgctggaagatgtgatcctcccctcctacgatgagctgaacaccatgctggaaacgcgccagcaaactctgttgctgccggaaagcgcggcggacgtggtggtgcgcggtgacgcgacgttactgcgtatgctgctgcgaaatctggtggaaaacgcgcatcgctatagccctgaaggaacccatatcactatccacattagcgccgaccccgacgctattatggcggtcgaagacgaggggccgggtattgatgaaagcaaatgcgggaagctaagcgaagcgttcgtgcggatggacagccgttatggcggaattggcctggggctgagtatcgtcagccgcatcacccagctacatcagggacagtttttcctgcaaaaccgtacggaaagaacaggcacccgtgcctgggtgctgttgaaaaaggcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z