BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898431 1 PolyA range1898431 1 1 9 annotation1898430 1 PolyA range1898430 1 32 39 annotation1898428 1 B1006 range1898428 1 1 39 annotation1898429 1 modified thr terminator range1898429 1 10 31 BBa_K1459004 1 BBa_K1459004 PmrA-PmrB(LBT) with terminator (BBa_B1006) 2014-09-30T11:00:00Z 2015-05-08T01:10:32Z PmrA-PmrB operon sequence was obtained thanks to kindness of prof. He from the University of Chicago. BBa_B1006 was obtained from 2014 Spring Distribution. PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions. false false _1837_ 0 20803 9 It's complicated false Not many, apart from ligating these two parts together. false Grzegorz Ścibisz component2391575 1 BBa_B1006 component2391570 1 BBa_K1459003 annotation2391575 1 BBa_B1006 range2391575 1 1758 1796 annotation2391570 1 BBa_K1459003 range2391570 1 1 1749 BBa_K1459003 1 BBa_K1459003 PmrA-PmrB(LBT) two-component system 2014-09-30T11:00:00Z 2015-05-08T01:10:32Z PmrA-PmrB operon sequence was obtained thanks to kindness of prof. He from the University of Chicago. PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions. false false _1837_ 0 20803 9 Not in stock false We had to only add BioBrick Preffix and Suffix. false Grzegorz Ścibisz BBa_K1459004_sequence 1 atgaagatactgattgttgaagacgacacgctattattacaggggttaatactcgccgcgcaaaccgaaggctatgcgtgtgatggcgtttcgacagcgcgtgccgccgagcatagtctggagtctggtcattacagtctgatggtgctggatttagggctgcccgatgaggatggcctgcatttcctgacgcgaatccgacagaaaaaatacaccctgccggtactcattctgaccgcccgcgatacgctcaacgaccgcattagcgggctggatgtcggcgcagatgattatctggtaaaacccttcgccctggaggagctgcacgcccgcatccgcgcgttgctgcgccgccataataaccagggtgaaagtgaactgacggttggcaatctgacgctcaatataggccgccatcaggcatggagggatggacaggaactgaccctgacgcctaaggagtacgcgctgctctcacgattgatgctcaaggcaggcagtccggtgcaccgggaaattctttataacgatatctacaactgggataacgaaccctcgaccaacactctggaagtgcatatacataatttgcgcgacaaagtcggcaagtcgcgcattcgcacggttcgcaggtttggctacatgctggttgccactgaggaaagctaagtgagcctgatgcgttttcagcgaagagcgatgacccttcgccagcgtttaacgctgacaattggtcttattctgctggtgttccagctaatcagtaccttctggttatggcatgaaagcactgagcaaatccaactgttcgagcaggcgctgcgggataatcgcaacaacgatcgccatatcatgcacgaaattcgcgaggcggtcgccagcctgatcgtccccagcgtatttatggttagcctgacgctgctggtttgctaccaggcggtacggcgtattacccgcccactcgccgaactgcaaaaagagctggaagcgcggacggcggataatctgacgccgatcgccattcacagctccacgcttgagattgagtccgtcgtctcggcgatcaatcaactggttacgcgtttgaccaccacgctcgacaatgaacgcctttttaccgccgatgtggcccatgagctacgcacgccgctgtcgggggtgcgtttgcatctggaattattgtcaaaaacccacaatgttgatgtcgcgccgcttatcgcccgtcttgaccagatgatggatagcgtctcccagcttctgcaactggcgcgcgtgggccagtcattctcttccgggaattatcaggaagtaaaactgctggaagatgtgatcctcccctcctacgatgagctgaacaccatgctggaaacgcgccagcaaactctgttgctgccggaaagcgcggcggacgtggtggtgcgcggtgacgcgacgttactgcgtatgctgctgcgaaatctggtggaaaacgcgcatcgctatagccctgaaggaacccatatcactatccacattagcgccgaccccgacgctattatggcggtcgaagacgaggggccgggtattgatgaaagcaaatgcgggaagctaagcgaagcgttcgtgcggatggacagccgttatggcggaattggcctggggctgagtatcgtcagccgcatcacccagctacatcagggacagtttttcctgcaaaaccgtacggaaagaacaggcacccgtgcctgggtgctgttgaaaaaggcataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K1459003_sequence 1 atgaagatactgattgttgaagacgacacgctattattacaggggttaatactcgccgcgcaaaccgaaggctatgcgtgtgatggcgtttcgacagcgcgtgccgccgagcatagtctggagtctggtcattacagtctgatggtgctggatttagggctgcccgatgaggatggcctgcatttcctgacgcgaatccgacagaaaaaatacaccctgccggtactcattctgaccgcccgcgatacgctcaacgaccgcattagcgggctggatgtcggcgcagatgattatctggtaaaacccttcgccctggaggagctgcacgcccgcatccgcgcgttgctgcgccgccataataaccagggtgaaagtgaactgacggttggcaatctgacgctcaatataggccgccatcaggcatggagggatggacaggaactgaccctgacgcctaaggagtacgcgctgctctcacgattgatgctcaaggcaggcagtccggtgcaccgggaaattctttataacgatatctacaactgggataacgaaccctcgaccaacactctggaagtgcatatacataatttgcgcgacaaagtcggcaagtcgcgcattcgcacggttcgcaggtttggctacatgctggttgccactgaggaaagctaagtgagcctgatgcgttttcagcgaagagcgatgacccttcgccagcgtttaacgctgacaattggtcttattctgctggtgttccagctaatcagtaccttctggttatggcatgaaagcactgagcaaatccaactgttcgagcaggcgctgcgggataatcgcaacaacgatcgccatatcatgcacgaaattcgcgaggcggtcgccagcctgatcgtccccagcgtatttatggttagcctgacgctgctggtttgctaccaggcggtacggcgtattacccgcccactcgccgaactgcaaaaagagctggaagcgcggacggcggataatctgacgccgatcgccattcacagctccacgcttgagattgagtccgtcgtctcggcgatcaatcaactggttacgcgtttgaccaccacgctcgacaatgaacgcctttttaccgccgatgtggcccatgagctacgcacgccgctgtcgggggtgcgtttgcatctggaattattgtcaaaaacccacaatgttgatgtcgcgccgcttatcgcccgtcttgaccagatgatggatagcgtctcccagcttctgcaactggcgcgcgtgggccagtcattctcttccgggaattatcaggaagtaaaactgctggaagatgtgatcctcccctcctacgatgagctgaacaccatgctggaaacgcgccagcaaactctgttgctgccggaaagcgcggcggacgtggtggtgcgcggtgacgcgacgttactgcgtatgctgctgcgaaatctggtggaaaacgcgcatcgctatagccctgaaggaacccatatcactatccacattagcgccgaccccgacgctattatggcggtcgaagacgaggggccgggtattgatgaaagcaaatgcgggaagctaagcgaagcgttcgtgcggatggacagccgttatggcggaattggcctggggctgagtatcgtcagccgcatcacccagctacatcagggacagtttttcctgcaaaaccgtacggaaagaacaggcacccgtgcctgggtgctgttgaaaaaggcataa BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z