BBa_K1459005
1
BBa_K1459005
PmrA-PmrB(N-term) from Salmonella
2014-10-06T11:00:00Z
2015-05-08T01:10:32Z
We obtained PmrA-PmrB sequences thanks to kindness of prof. He from the University of Chicago.
This is N-terminal part of PmrA-PmrB two-component system native to Salmonella enterica.
PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions.
In this part, PmrB protein is truncated just before iron binding tag, which enables one to put any desired tag between two parts of PmrB, to construct a detecting system based on PmrA-PmrB system.
false
false
_1837_
0
20803
9
It's complicated
false
We had to truncate PmrB sequence just before the iron binding tag.
false
Grzegorz Ścibisz
BBa_K1459005_sequence
1
atgaagatactgattgttgaagacgacacgctattattacaggggttaatactcgccgcgcaaaccgaaggctatgcgtgtgatggcgtttcgacagcgcgtgccgccgagcatagtctggagtctggtcattacagtctgatggtgctggatttagggctgcccgatgaggatggcctgcatttcctgacgcgaatccgacagaaaaaatacaccctgccggtactcattctgaccgcccgcgatacgctcaacgaccgcattagcgggctggatgtcggcgcagatgattatctggtaaaacccttcgccctggaggagctgcacgcccgcatccgcgcgttgctgcgccgccataataaccagggtgaaagtgaactgacggttggcaatctgacgctcaatataggccgccatcaggcatggagggatggacaggaactgaccctgacgcctaaggagtacgcgctgctctcacgattgatgctcaaggcaggcagtccggtgcaccgggaaattctttataacgatatctacaactgggataacgaaccctcgaccaacactctggaagtgcatatacataatttgcgcgacaaagtcggcaagtcgcgcattcgcacggttcgcaggtttggctacatgctggttgccactgaggaaagctaagtgagcctgatgcgttttcagcgaagagcgatgacccttcgccagcgtttaacgctgacaattggtcttattctgctggtgttccagctaatcagtaccttctggttatgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z