BBa_K1459009
1
BBa_K1459009
PmrB(C-term)
2014-10-06T11:00:00Z
2015-05-08T01:10:32Z
We obtained PmrA-PmrB system thanks to kindness of prof. He from the University of Chicago.
PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein.
This part is 'functionally complementary' to PmrA-PmrB(N-term) (BBa_K1459005), in a way that construction of theoretically any detection system is possible by fusing PmrA-PmrB(N-term) with PmrB(C-term) with any binding tag in between two protein domains.
false
false
_1837_
0
20803
9
Not in stock
false
This part is, much like PmrA-PmrB(N-term), truncated after iron binding tag in PmrB sequence.
false
Grzegorz Ścibisz
BBa_K1459009_sequence
1
gaggcggtcgccagcctgatcgtccccagcgtatttatggttagcctgacgctgctggtttgctaccaggcggtacggcgtattacccgcccactcgccgaactgcaaaaagagctggaagcgcggacggcggataatctgacgccgatcgccattcacagctccacgcttgagattgagtccgtcgtctcggcgatcaatcaactggttacgcgtttgaccaccacgctcgacaatgaacgcctttttaccgccgatgtggcccatgagctacgcacgccgctgtcgggggtgcgtttgcatctggaattattgtcaaaaacccacaatgttgatgtcgcgccgcttatcgcccgtcttgaccagatgatggatagcgtctcccagcttctgcaactggcgcgcgtgggccagtcattctcttccgggaattatcaggaagtaaaactgctggaagatgtgatcctcccctcctacgatgagctgaacaccatgctggaaacgcgccagcaaactctgttgctgccggaaagcgcggcggacgtggtggtgcgcggtgacgcgacgttactgcgtatgctgctgcgaaatctggtggaaaacgcgcatcgctatagccctgaaggaacccatatcactatccacattagcgccgaccccgacgctattatggcggtcgaagacgaggggccgggtattgatgaaagcaaatgcgggaagctaagcgaagcgttcgtgcggatggacagccgttatggcggaattggcctggggctgagtatcgtcagccgcatcacccagctacatcagggacagtttttcctgcaaaaccgtacggaaagaacaggcacccgtgcctgggtgctgttgaaaaaggcataat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z