BBa_K1459009 1 BBa_K1459009 PmrB(C-term) 2014-10-06T11:00:00Z 2015-05-08T01:10:32Z We obtained PmrA-PmrB system thanks to kindness of prof. He from the University of Chicago. PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. This part is 'functionally complementary' to PmrA-PmrB(N-term) (BBa_K1459005), in a way that construction of theoretically any detection system is possible by fusing PmrA-PmrB(N-term) with PmrB(C-term) with any binding tag in between two protein domains. false false _1837_ 0 20803 9 Not in stock false This part is, much like PmrA-PmrB(N-term), truncated after iron binding tag in PmrB sequence. false Grzegorz Ścibisz BBa_K1459009_sequence 1 gaggcggtcgccagcctgatcgtccccagcgtatttatggttagcctgacgctgctggtttgctaccaggcggtacggcgtattacccgcccactcgccgaactgcaaaaagagctggaagcgcggacggcggataatctgacgccgatcgccattcacagctccacgcttgagattgagtccgtcgtctcggcgatcaatcaactggttacgcgtttgaccaccacgctcgacaatgaacgcctttttaccgccgatgtggcccatgagctacgcacgccgctgtcgggggtgcgtttgcatctggaattattgtcaaaaacccacaatgttgatgtcgcgccgcttatcgcccgtcttgaccagatgatggatagcgtctcccagcttctgcaactggcgcgcgtgggccagtcattctcttccgggaattatcaggaagtaaaactgctggaagatgtgatcctcccctcctacgatgagctgaacaccatgctggaaacgcgccagcaaactctgttgctgccggaaagcgcggcggacgtggtggtgcgcggtgacgcgacgttactgcgtatgctgctgcgaaatctggtggaaaacgcgcatcgctatagccctgaaggaacccatatcactatccacattagcgccgaccccgacgctattatggcggtcgaagacgaggggccgggtattgatgaaagcaaatgcgggaagctaagcgaagcgttcgtgcggatggacagccgttatggcggaattggcctggggctgagtatcgtcagccgcatcacccagctacatcagggacagtttttcctgcaaaaccgtacggaaagaacaggcacccgtgcctgggtgctgttgaaaaaggcataat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z