BBa_K1459010 1 BBa_K1459010 PmrB(LBT) from Salmonella 2014-10-06T11:00:00Z 2015-05-08T01:10:32Z We obtained PmrA-PmrB sequences thanks to kindness of prof. He from the University of Chicago. PmrB(LBT) is a engineered PmrB gene, in which iron binding tag was replaced by lanthanide binding tag. PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions. false false _1837_ 0 20803 9 It's complicated false Only adding BioBrick Preffix and Suffix. false Grzegorz Ścibisz BBa_K1459010_sequence 1 atgcgttttcagcgaagagcgatgacccttcgccagcgtttaatgctgacaattggtcttattctgctggtgttccagttaatcagtaccttctggttaggctacatcgataccaacaatgacggctggatcgaaggtgatgaactcgaggcggtcgccagcctgatcgtccccggcgtatttatggttagcctgacgctgctaatttgttaccaggcggtacggcgtattacccgtccgctcgccgaactgcaaaaagagctggaagcgcggacggcggataatctggcgccgatcgccattcacagctccacgcttgagattgagtccgtcgtctcggcgatcaatcaactggttacgcgtttgaccaccacgctcgacaatgaacgcctttttaccgccgatgtggcccatgagctacgcacgccgctgtcgggggtgcgtttgcacctggaattattgtcaaaaacccacaatgttgatgtcgcgccgcttatcgcccgtcttgaccagatgatggatagcgtctcccagcttctgcaactggcgcgcgtgggccagtcattctcttccgggaattatcaggaagtaaaactgctggaagatgtgatcctcccctcctacgatgagctgaacaccatgctggaaacgcgccagcaaactctgttgctgccggaaagcgcggcggacgtggtggtgcgcggtgatgcgacgttactgcgtatgctgttgcgaaatctggtggaaaacgcgcatcgctatagccctgaaggaacccatatcactatccacattagcgccgaccccgacgctattatggcggtcgaagacgaggggccgggtattgatgaaagcaaatgcgggaagctaagcgaagcgttcgtgcggatggacagccgttatggcggaattggcctggggctgagtatcgtcagccgcatcacccagctacatcagggacagtttttcctgcaaaaccgtacggaaagaacaggcacccgtgcctgggtgctgttgaaaaaggcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z