BBa_K1459010
1
BBa_K1459010
PmrB(LBT) from Salmonella
2014-10-06T11:00:00Z
2015-05-08T01:10:32Z
We obtained PmrA-PmrB sequences thanks to kindness of prof. He from the University of Chicago.
PmrB(LBT) is a engineered PmrB gene, in which iron binding tag was replaced by lanthanide binding tag.
PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions.
false
false
_1837_
0
20803
9
It's complicated
false
Only adding BioBrick Preffix and Suffix.
false
Grzegorz Ścibisz
BBa_K1459010_sequence
1
atgcgttttcagcgaagagcgatgacccttcgccagcgtttaatgctgacaattggtcttattctgctggtgttccagttaatcagtaccttctggttaggctacatcgataccaacaatgacggctggatcgaaggtgatgaactcgaggcggtcgccagcctgatcgtccccggcgtatttatggttagcctgacgctgctaatttgttaccaggcggtacggcgtattacccgtccgctcgccgaactgcaaaaagagctggaagcgcggacggcggataatctggcgccgatcgccattcacagctccacgcttgagattgagtccgtcgtctcggcgatcaatcaactggttacgcgtttgaccaccacgctcgacaatgaacgcctttttaccgccgatgtggcccatgagctacgcacgccgctgtcgggggtgcgtttgcacctggaattattgtcaaaaacccacaatgttgatgtcgcgccgcttatcgcccgtcttgaccagatgatggatagcgtctcccagcttctgcaactggcgcgcgtgggccagtcattctcttccgggaattatcaggaagtaaaactgctggaagatgtgatcctcccctcctacgatgagctgaacaccatgctggaaacgcgccagcaaactctgttgctgccggaaagcgcggcggacgtggtggtgcgcggtgatgcgacgttactgcgtatgctgttgcgaaatctggtggaaaacgcgcatcgctatagccctgaaggaacccatatcactatccacattagcgccgaccccgacgctattatggcggtcgaagacgaggggccgggtattgatgaaagcaaatgcgggaagctaagcgaagcgttcgtgcggatggacagccgttatggcggaattggcctggggctgagtatcgtcagccgcatcacccagctacatcagggacagtttttcctgcaaaaccgtacggaaagaacaggcacccgtgcctgggtgctgttgaaaaaggcataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z