BBa_K1459011
1
BBa_K1459011
PmrB(N-term)
2014-10-06T11:00:00Z
2015-05-08T01:10:32Z
We obtained PmrA-PmrB sequences thanks to kindness of prof. He of the University of Chicago.
PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions.
This part was truncated just before the iron binding tag, and PmrB(N-term) is functionally complementar to PmrB(C-term).
false
false
_1837_
0
20803
9
In stock
false
We had to truncate the sequence of PmrB just before start of iron binding tag on the extracellular loop of PmrB.
false
Grzegorz Ścibisz
BBa_K1459011_sequence
1
atgcgttttcagcgaagagcgatgacccttcgccagcgtttaatgctgacaattggtcttattctgctggtgttccagttaatcagtaccttctggttatgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z