BBa_K1460007 1 BBa_K1460007 Anderson Promoter + merT + merP + Ter + pT7 + GST-CRS5 + Ter 2014-08-25T11:00:00Z 2016-01-13T10:19:27Z This part is a composite of parts BBa_K1460004 and BBa_K1460001 This composite part functionally codes for the selective mercury transporters merT and merP as well as the metallothioniein CRS5 fused to glutathione-S-transferase. This composite part should act to transport mercury ions into the engineered cell where they will be sequestered by the metallothionein allowing for Hg²⁺ removal from contaminated water. false false _1839_ 4206 15649 9 Not in stock false Expression of GST-CRS5 is controlled by the bacteriophage T7 promoter, so part should be combined with a host organism expressing T7 polymerase (such as <i>E. coli</i> BL21-AI from Life Technologies). false Eric Holmes component2381571 1 BBa_K1460004 component2381578 1 BBa_K1460001 annotation2381578 1 BBa_K1460001 range2381578 1 750 1777 annotation2381571 1 BBa_K1460004 range2381571 1 1 741 BBa_K1460004 1 BBa_K1460004 Anderson Promoter + MerT + MerP + ter 2014-08-25T11:00:00Z 2016-01-13T10:25:46Z Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. merT/merP genes are naturally found in Pseudomonas aeruginosa. This part was synthesized by GenScript in the vector pUC57. The part was then transferred into pSB1C3 using standard cloning procedures. This is a functional coding sequence for the mercury transport proteins merT/merP from P. aeruginosa. This part will result in mercury intolerance due to greater mercury transport into engineered cells. false false _1839_ 4206 15649 9 In stock false The Anderson promoter provides strong constitutive expression of merT/merP in E. coli false Eric Holmes annotation2381485 1 merT range2381485 1 63 413 annotation2381483 1 BBa_J61101 range2381483 1 43 56 annotation2381484 1 BBa_J23102 range2381484 1 1 35 annotation2381487 1 BBa_B1006 range2381487 1 702 740 annotation2381486 1 merP range2381486 1 426 701 BBa_K1460001 1 BBa_K1460001 pT7 + RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter 2014-08-13T11:00:00Z 2016-12-03T10:52:20Z T7 promoter is part BBa_I712074. RBS is from part BBa_J61101. Glutathione-S-transferase is from S. japonicum. CRS5 is a metallothionein from S. cerevisiae. Terminator is part BBa_B1006. This sequence was synthesized by GenScript in the vector pUC57 and was then transferred using traditional cloning into pSB1C3. This part serves as a functional metallothionein for heavy metal binding and conferred resistance to heavy metals when combined with a strain expressing the bacteriophage T7 polymerase. CRS5 gene is the metallothionein that binds to heavy metals and it is fused to GST for stability. false false _1839_ 24251 15649 9 In stock true Flanking the T7 promoter are two KpnI cut sites with both the T7 promoter and a SacI cut site between them. These cut sites are present for easy removal of the T7 promoter for modulation of parts with other promoters. The SacI cut site can be used as a marker for successful removal of the T7 promoter from the part. When digested with SacI, part BBa_K1460001 in pSB1C3 will yield bands of size 2166bp and 931bp. If the T7 promoter is successfully removed, when digested with SacI the plasmid will yield the linearized length of the pSB1C3+BBa_K1460001 false Eric Holmes annotation2380961 1 BBa_J61101 range2380961 1 72 85 annotation2380965 1 BBa_B1006 range2380965 1 989 1027 annotation2380963 1 Linker Sequence range2380963 1 746 778 annotation2380960 1 T7 Promoter range2380960 1 13 59 annotation2380962 1 GST range2380962 1 92 745 annotation2380964 1 CRS5 range2380964 1 779 988 BBa_K1460004_sequence 1 ttgacagctagctcagtcctaggtactgtgctagctactagagaaagacaggaccctactagatgtctgaaccaaaaaccgggcgcggcgcgctcttcactggagggcttgccgccatcctcgcctcggcttgctgcctcgggccgttggttctgatcgccttggggttcagcggcgcttggatcggcaacttggcggtgttggaaccctatcgccccatctttatcggcgtggcgctggtggcgttgttcttcgcctggcggcgcatctaccggcaggcagcggcctgcaaaccgggtgaggtctgcgcgattccccaagtgcgagctacttacaagctcattttctggatcgtggccgcgctggttctggtcgcgctcggatttccctacgtcatgccatttttctactgatcggagttcaccatgaagaaactgtttgcctccctcgccctcgccgccgttgttgcccccgtctgggccgccacccagaccgtcacgctgtccgtaccgggcatgacctgctccgcctgcccgatcactgtcaagaaggcgatttccgaggtcgaaggcgtcagcaaagttgacgtgactttcgagacacgccaagcggtcgtcaccttcgacgatgccaagaccagcgtgcagaagctgaccaaggcaaccgcagacgcgggctatccgtccagcgtcaagcagtgaaaaaaaaaaccccgcccctgacagggcggggttttttttt BBa_K1460001_sequence 1 gtaccgagctcgtaatacgactcactatagggaatacaagctacttgttctttttgcaggtacctactagagaaagacaggaccctactagatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggttccgcgtggatccattcatatgatgactgtaaaaatatgtgactgtgaaggcgaatgttgtaaggactcttgtcattgtgggagcacctgccttccaagctgttctggcggtgaaaagtgcaaatgtgatcacagcaccggaagccctcaatgtaagagttgtggtgaaaaatgcaaatgcgaaaccacgtgcacttgtgaaaagagtaaatgcaattgtgaaaaatgttagaaaaaaaaaccccgcccctgacagggcggggttttttttt BBa_K1460007_sequence 1 ttgacagctagctcagtcctaggtactgtgctagctactagagaaagacaggaccctactagatgtctgaaccaaaaaccgggcgcggcgcgctcttcactggagggcttgccgccatcctcgcctcggcttgctgcctcgggccgttggttctgatcgccttggggttcagcggcgcttggatcggcaacttggcggtgttggaaccctatcgccccatctttatcggcgtggcgctggtggcgttgttcttcgcctggcggcgcatctaccggcaggcagcggcctgcaaaccgggtgaggtctgcgcgattccccaagtgcgagctacttacaagctcattttctggatcgtggccgcgctggttctggtcgcgctcggatttccctacgtcatgccatttttctactgatcggagttcaccatgaagaaactgtttgcctccctcgccctcgccgccgttgttgcccccgtctgggccgccacccagaccgtcacgctgtccgtaccgggcatgacctgctccgcctgcccgatcactgtcaagaaggcgatttccgaggtcgaaggcgtcagcaaagttgacgtgactttcgagacacgccaagcggtcgtcaccttcgacgatgccaagaccagcgtgcagaagctgaccaaggcaaccgcagacgcgggctatccgtccagcgtcaagcagtgaaaaaaaaaaccccgcccctgacagggcggggttttttttttactagaggtaccgagctcgtaatacgactcactatagggaatacaagctacttgttctttttgcaggtacctactagagaaagacaggaccctactagatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggttccgcgtggatccattcatatgatgactgtaaaaatatgtgactgtgaaggcgaatgttgtaaggactcttgtcattgtgggagcacctgccttccaagctgttctggcggtgaaaagtgcaaatgtgatcacagcaccggaagccctcaatgtaagagttgtggtgaaaaatgcaaatgcgaaaccacgtgcacttgtgaaaagagtaaatgcaattgtgaaaaatgttagaaaaaaaaaccccgcccctgacagggcggggttttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z