LOCUS       BBa_K1460008            3225 bp    DNA     linear       26-Nov-2024
DEFINITION  Anderson Promoter + CBP4 + pT7 + GST-CRS5 + Ter
ACCESSION   BBa_K1460008
VERSION     BBa_K1460008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     RBS             43..56
                     /label=BBa_J61101
     CDS             63..2189
                     /label=CBP4
     misc_feature    1..2189
                     /label=BBa_K1460005
     promoter        2210..2256
                     /label=T7 Promoter
     RBS             2269..2282
                     /label=BBa_J61101
     CDS             2289..2942
                     /label=GST
     misc_feature    2943..2975
                     /label=Linker Sequence
     CDS             2976..3185
                     /label=CRS5
     misc_feature    3186..3224
                     /label=BBa_B1006
     misc_feature    2198..3225
                     /label=BBa_K1460001
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaaagaca ggaccctact
       61 agatgaatca ccgccaagac gagtttgtga ggtttcagga ttggaaatca gagagaagct
      121 ctgagggaaa ttttcatgct aaaggtggag tccaccgaag taaagttgta atagcttccg
      181 acgaattaca taatcgcttg gaatctggta aatggagggc caaaggcata atacaagctg
      241 taaaatctag tttaagtggt tttgtggaag aaagcttggg atccaaaaag aacattcttg
      301 atcctcaagg accttttctt cgaaagtgga acaaaatatt tgtattatcc tgtgtaattg
      361 ctatctcctt ggatcctttg ttcctttata ttccagtgat tgataacgac aacaaatgcc
      421 tggggttaaa cagaacgttg gaggtgacag ctagtgtttt gcgttctttt actgatatat
      481 tttactttct tcatattgcc cttcaatttc gaactggttt tattgctcct tcgtcacgtg
      541 tatttggaag aggtgtttta attgaagatg cttgggagat agcaaagaga tacttatcca
      601 cttacttctt gatcgacatt cttgcagttc taccactacc acaggttgta attttaatta
      661 taattcctaa gttgcggggt tcaagatctt tgaataccaa gaatttgctg aagtctgttg
      721 tctttttcca atatattcca agggtccttc gagtgtatcc tttatatagg gaagtcacaa
      781 gaacatctgg catactcact gaaacagcat gggctggagc tgctttcaat ctcttccttt
      841 atatgcttgc cagccatgta cttggagcct tttggtatct gttttctata gaacgcgaaa
      901 ctacttgctg gaaacaagct tgtggaaatt cttctccctg tcatcatgcc tcattgtatt
      961 gtgatgatga tcatacaaaa tttaaaacat tgctgaacag ttcctgccct atagagacac
     1021 caaatgcaac gctatttgat tttgggatat tccttggcgc tctccaatca ggtgttgtgg
     1081 gaccaatgga ttttccgcag aagttctttt attgtttctg gtggggttta caaaatttaa
     1141 gttcccttgg tcaaaacctc caaacaagta ccttcatctg ggaaatgtgc tttgcagttt
     1201 tcatttccat cgctggcctg gtgctatttg cctttctcat tggcaatatg cagacatgtc
     1261 tgcaatcctc aacattaaga ttagaggaga tgagggtgaa aagacgagat gcagaacagt
     1321 ggatgtcaca tcggttgctc cctgagaacc tcagagagcg gattaggtgc tatgagcaat
     1381 acaggtggca ggaaactagg ggtgttgatg aagagaatct gattcacaac cttccgaaag
     1441 acctcaggag agatataaag cgtcatcttt gtttagcttt gctcatgaga gtgcccatgt
     1501 ttgaaaaaat ggatgaacaa ctcttagatg cattatgcga ccatctgaag ccagtgctat
     1561 tcaccaagga cagcttcatt gttcgtgaag gtgatccagt tgatgcgatg ctgtttgtaa
     1621 tgaggggcaa actactgtct gtaaccacta atggagggag gacaggtttt tttaactctg
     1681 agcatctaaa agctggtgat ttttgtggag aggagctact tacttgggct cttgacccta
     1741 attcgtcaac taacctacca atctcaacta gaactgccca agcactctca gaagttgaag
     1801 catttgcttt agtggctgat gatttaaagc ttgtagcctc tcagttcaga cgacttcaca
     1861 gtaagcagct ccgccacact ttcaggtttt actcaggcca atggagaact tgggcagctt
     1921 gcttcataca agcagcatgg cggagttatt gcaggaagaa tgtagaagag tctcttcgtg
     1981 atgaagaaaa caggttgcaa gatgcattgg caaatgaagg tggcagttca ccaagtttgg
     2041 gtgctacctt ttatgcatca agatttgctg ctaatgtact ccatgctttg cggcgcaata
     2101 ctgcaaaaaa agcaagggtg ccagacagga tatcacccat tctgcttcag aagccaacag
     2161 agccagattt tactgctgaa gataattaat actagaggta ccgagctcgt aatacgactc
     2221 actataggga atacaagcta cttgttcttt ttgcaggtac ctactagaga aagacaggac
     2281 cctactagat gtcccctata ctaggttatt ggaaaattaa gggccttgtg caacccactc
     2341 gacttctttt ggaatatctt gaagaaaaat atgaagagca tttgtatgag cgcgatgaag
     2401 gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat cttccttatt
     2461 atattgatgg tgatgttaaa ttaacacagt ctatggccat catacgttat atagctgaca
     2521 agcacaacat gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag
     2581 cggttttgga tattagatac ggtgtttcga gaattgcata tagtaaagac tttgaaactc
     2641 tcaaagttga ttttcttagc aagctacctg aaatgctgaa aatgttcgaa gatcgtttat
     2701 gtcataaaac atatttaaat ggtgatcatg taacccatcc tgacttcatg ttgtatgacg
     2761 ctcttgatgt tgttttatac atggacccaa tgtgcctgga tgcgttccca aaattagttt
     2821 gttttaaaaa acgtattgaa gctatcccac aaattgataa gtacttgaaa tccagcaagt
     2881 atatagcatg gcctttgcag ggctggcaag ccacgtttgg tggtggcgac catcctccaa
     2941 aatcggatct ggttccgcgt ggatccattc atatgatgac tgtaaaaata tgtgactgtg
     3001 aaggcgaatg ttgtaaggac tcttgtcatt gtgggagcac ctgccttcca agctgttctg
     3061 gcggtgaaaa gtgcaaatgt gatcacagca ccggaagccc tcaatgtaag agttgtggtg
     3121 aaaaatgcaa atgcgaaacc acgtgcactt gtgaaaagag taaatgcaat tgtgaaaaat
     3181 gttagaaaaa aaaaccccgc ccctgacagg gcggggtttt ttttt
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