LOCUS BBa_K146001 3823 bp DNA linear 16-Feb-2025 DEFINITION AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002 ACCESSION BBa_K146001 VERSION BBa_K146001.1 FEATURES Location/Qualifiers misc_feature 1..867 /label=BioBrick primer_bind 1..19 /label=primer_binding primer_bind 844..867 /label=primer_binding promoter 1..867 /label=BBa_I764001 promoter 3603..3631 /label=beta-lactamase (ampR, bla) promoter CDS 902..1582 /label=mRFP misc_feature 1616..1744 /label=BBa_B0015 misc_feature 1924..2606 /label=ColE1 origin CDS 2701..3561 /label=beta-lactamase ampicillin resistance marker stem_loop 1627..1670 /label=rrnB terminator misc_feature 1927..2546 /label=pBR322 origin misc_feature 3802..3823 /label=BioBrick prefix misc_feature 877..882 /label=SpeI misc_feature 1760..1765 /label=PstI misc_feature 1753..1760 /label=NotI RBS 884..895 /label=RBS misc_feature 884..895 /label=BBa_B0034 RBS primer_bind 1823..1842 /label=Verification reverse (VR) primer binding site primer_bind 3684..3703 /label=Verification forward (VF2) primer binding site misc_feature 3816..3816 /label=Ambiguity; should be a T misc_feature 876..3823 /label=BBa_J61002 ORIGIN 1 catccgacat ccacaggtcc attctcacac ataagtgcca aacgcaacag gaggggatac 61 actagcagca gaccgttgca aacgcaggac ctccactcct cttctcctca acacccactt 121 ttgccatcga aaaaccagcc cagttattgg gcttgattgg agctcgctca ttccaattcc 181 ttctattagg ctactaacac catgacttta ttagcctgtc tatcctggcc cccctggcga 241 ggttcatgtt tgtttatttc cgaatgcaac aagctccgca ttacacccga acatcactcc 301 agatgagggc tttctgagtg tggggtcaaa tagtttcatg ttccccaaat ggcccaaaac 361 tgacagttta aacgctgtct tggaacctaa tatgacaaaa gcgtgatctc atccaagatg 421 aactaagttt ggttcgttga aatgctaacg gccagttggt caaaaagaaa cttccaaaag 481 tcgccatacc gtttgtcttg tttggtattg attgacgaat gctcaaaaat aatctcatta 541 atgcttagcg cagtctctct atcgcttctg aaccccggtg cacctgtgcc gaaacgcaaa 601 tggggaaaca cccgcttttt ggatgattat gcattgtctc cacattgtat gcttccaaga 661 ttctggtggg aatactgctg atagcctaac gttcatgatc aaaatttaac tgttctaacc 721 cctacttgac agcaatatat aaacagaagg aagctgccct gtcttaaacc ttttttttta 781 tcatcattat tagcttactt tcataattgc gactggttcc aattgacaag cttttgattt 841 taacgacttt taacgacaac ttgagaatac tagagtacta gtgaaagagg agaaatacta 901 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg 961 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg 1021 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat 1081 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc 1141 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga 1201 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta 1261 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac 1321 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga 1381 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac 1441 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga 1501 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg 1561 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg 1621 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1681 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 1741 tatatactag aagcggccgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt 1801 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 1861 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 1921 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 1981 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2041 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2101 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2161 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 2221 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 2281 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 2341 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 2401 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 2461 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 2521 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 2581 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 2641 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 2701 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 2761 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 2821 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 2881 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 2941 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 3001 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 3061 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 3121 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 3181 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 3241 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 3301 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 3361 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 3421 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 3481 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 3541 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 3601 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 3661 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 3721 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc 3781 tttcgctaag gatgatttct ggaattcgcg gccgcatcta gag //