##gff-version 3
##sequence-region BBa_K146001 1 3823
BBa_K146001	.	plasmid_vector	876	3823	.	+	0	ID=BBa_J61002;Name=BBa_J61002
BBa_K146001	.	CDS	902	1582	.	+	0	ID=annotation1892273;Name=mRFP;Parent=BBa_J61002
BBa_K146001	.	sequence_feature	1753	1760	.	+	0	ID=annotation1975718;Name=NotI;Parent=BBa_J61002
BBa_K146001	.	primer_binding_site	3684	3703	.	+	0	ID=annotation1975769;Name=Verification forward (VF2) primer binding site;Parent=BBa_J61002
BBa_K146001	.	ribosome_entry_site	884	895	.	+	0	ID=annotation1975766;Name=RBS;Parent=BBa_J61002
BBa_K146001	.	sequence_alteration	3816	3816	.	+	0	ID=annotation1975921;Name=Ambiguity; should be a T;Parent=BBa_J61002
BBa_K146001	.	CDS	2701	3561	.	+	0	ID=annotation1892276;Name=beta-lactamase ampicillin resistance marker;Parent=BBa_J61002
BBa_K146001	.	engineered_region	1616	1744	.	+	0	ID=annotation1892274;Name=BBa_B0015;Parent=BBa_J61002
BBa_K146001	.	stem_loop	1627	1670	.	+	0	ID=annotation1975713;Name=rrnB terminator;Parent=BBa_J61002
BBa_K146001	.	sequence_feature	877	882	.	+	0	ID=annotation1975716;Name=SpeI;Parent=BBa_J61002
BBa_K146001	.	promoter	3603	3631	.	+	0	ID=annotation1892272;Name=beta-lactamase (ampR, bla) promoter;Parent=BBa_J61002
BBa_K146001	.	sequence_feature	1927	2546	.	+	0	ID=annotation1975714;Name=pBR322 origin;Parent=BBa_J61002
BBa_K146001	.	primer_binding_site	1823	1842	.	+	0	ID=annotation1975768;Name=Verification reverse (VR) primer binding site;Parent=BBa_J61002
BBa_K146001	.	sequence_feature	1924	2606	.	+	0	ID=annotation1892275;Name=ColE1 origin;Parent=BBa_J61002
BBa_K146001	.	engineered_region	884	895	.	+	0	ID=annotation1975767;Name=BBa_B0034 RBS;Parent=BBa_J61002
BBa_K146001	.	sequence_feature	1760	1765	.	+	0	ID=annotation1975717;Name=PstI;Parent=BBa_J61002
BBa_K146001	.	sequence_feature	3802	3823	.	+	0	ID=annotation1975715;Name=BioBrick prefix;Parent=BBa_J61002
BBa_K146001	.	promoter	1	867	.	+	0	ID=BBa_I764001;Name=BBa_I764001
BBa_K146001	.	engineered_region	1	867	.	+	0	ID=annotation1943748;Name=BioBrick;Parent=BBa_I764001
BBa_K146001	.	primer_binding_site	844	867	.	+	0	ID=annotation1943750;Name=primer_binding;Parent=BBa_I764001
BBa_K146001	.	primer_binding_site	1	19	.	+	0	ID=annotation1943749;Name=primer_binding;Parent=BBa_I764001
>BBa_K146001
catccgacatccacaggtccattctcacacataagtgccaaacgcaacaggaggggatacactagcagcagaccgttgc
aaacgcaggacctccactcctcttctcctcaacacccacttttgccatcgaaaaaccagcccagttattgggcttgatt
ggagctcgctcattccaattccttctattaggctactaacaccatgactttattagcctgtctatcctggcccccctgg
cgaggttcatgtttgtttatttccgaatgcaacaagctccgcattacacccgaacatcactccagatgagggctttctg
agtgtggggtcaaatagtttcatgttccccaaatggcccaaaactgacagtttaaacgctgtcttggaacctaatatga
caaaagcgtgatctcatccaagatgaactaagtttggttcgttgaaatgctaacggccagttggtcaaaaagaaacttc
caaaagtcgccataccgtttgtcttgtttggtattgattgacgaatgctcaaaaataatctcattaatgcttagcgcag
tctctctatcgcttctgaaccccggtgcacctgtgccgaaacgcaaatggggaaacacccgctttttggatgattatgc
attgtctccacattgtatgcttccaagattctggtgggaatactgctgatagcctaacgttcatgatcaaaatttaact
gttctaacccctacttgacagcaatatataaacagaaggaagctgccctgtcttaaacctttttttttatcatcattat
tagcttactttcataattgcgactggttccaattgacaagcttttgattttaacgacttttaacgacaacttgagaata
ctagagtactagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagt
tcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccag
accgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggtt
ccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacg
tgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaa
gttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccg
aacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacga
cgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggac
atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaat
aacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagaagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcgg
tatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaagg
ccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcac
aaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccc
tcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttc
tcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgtt
cagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcag
cagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacgg
ctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttga
tccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctc
aagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgag
attatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaa
acttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttg
cctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgaga
cccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaact
ttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacg
ttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatc
aaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaag
ttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgctttt
ctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaat
acgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctca
aggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttca
ccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaat
actcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgt
atttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattatta
tcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaagga
tgatttctggaattcgcggccgcatctagag