LOCUS       BBa_K1461201             737 bp    DNA     linear       16-Dec-2024
DEFINITION  ecf20 (+RBS, +d.term)
ACCESSION   BBa_K1461201
VERSION     BBa_K1461201.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..600
                     /label=BBa_K1461004
     stem_loop       620..663
                     /label=stem_loop
     misc_feature    609..688
                     /label=BBa_B0010
     terminator      609..688
                     /label=BBa_B0010
     stem_loop       704..723
                     /label=T7 TE
     misc_feature    730..730
                     /label=stop
     polyA_site      724..737
                     /label=polya
     misc_feature    697..737
                     /label=BBa_B0012
     terminator      697..737
                     /label=BBa_B0012
     terminator      609..737
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gaacgaaacc gatccggatc tggaactgct gaaacgtatt
       61 ggcaacaacg atgcacaggc ggtgaaagaa atggtgaccc gcaaactgcc gcgcctgctg
      121 gcgctggcga gccgtctgct gggcgatgcg gatgaagcgc gcgatattgc gcaggaaagc
      181 tttctgcgca tttggaaaca ggcagcgagc tggcgcagcg aacaggcgcg ctttgatacc
      241 tggctgcatc gcgtggcgct gaacctgtgc tatgatcgcc tgcgccgtcg taaagaacat
      301 gtgccggtgg atagcgaaca tgcgtgcgaa gcgctggata cccgcccggc gccggatgaa
      361 cagctggaag cgagcgcgca gagccgtcgc atggcgcagg cactggatca gctgccggat
      421 cgtcagcgcg aagcgattgt gctgcaatat tatcaggaac tgagcaacac cgaagcagcg
      481 gcactgatgc agattagcgt ggaagcgctg gaaagcctgc tgagccgcgc acgtcgcaac
      541 ctgcgcagcc atctggcgga agcgccgggt gcggatctga gcggccgtcg caaaccgtaa
      601 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      661 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      721 gcctttctgc gtttata
//