BBa_K1461201 1 BBa_K1461201 ecf20 (+RBS, +d.term) 2014-09-24T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of RBS (BBa_B0034), ecf20 (BBa_K1461004), and double terminator (BBa_B0015). false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2386276 1 BBa_B0015 component2386268 1 BBa_B0034 component2386269 1 BBa_K1461004 annotation2386268 1 BBa_B0034 range2386268 1 1 12 annotation2386269 1 BBa_K1461004 range2386269 1 19 600 annotation2386276 1 BBa_B0015 range2386276 1 609 737 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1461004 1 BBa_K1461004 ecf20 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Fluorescens. This part is from Pseudomonas Fluorescens sigma 70 family. ecf20 is a sigma factor which activates ecf20 promoter (BBa_K1461001). false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1461201_sequence 1 aaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K1461004_sequence 1 atgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z