BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1461006
1
BBa_K1461006
anti ecf20
2014-09-23T11:00:00Z
2015-05-08T01:10:33Z
Pseudomonas Fluorescens.
This part is an anti-sigma factor. Anti ecf20 prevents ecf20 from activating ecf20 promoter.
false
false
_1840_
0
18036
9
Not in stock
true
None.
false
Takefumi Yoshikawa
BBa_K1461206
1
BBa_K1461206
anti ecf20 (+RBS, +d.term)
2014-09-25T11:00:00Z
2015-05-08T01:10:33Z
Assembly.
This part is a composite part of RBS (BBa_B0034), anti ecf20 (BBa_K1461006), and double terminator (BBa_B0015).
false
false
_1840_
0
18036
9
It's complicated
false
None.
false
Takefumi Yoshikawa
component2386622
1
BBa_B0015
component2386615
1
BBa_K1461006
component2386614
1
BBa_B0034
annotation2386614
1
BBa_B0034
range2386614
1
1
12
annotation2386615
1
BBa_K1461006
range2386615
1
19
450
annotation2386622
1
BBa_B0015
range2386622
1
459
587
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1461006_sequence
1
atgaccccggaacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaa
BBa_K1461206_sequence
1
aaagaggagaaatactagatgaccccggaacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z