BBa_K1461000 1 BBa_K1461000 crRBS 2014-09-21T11:00:00Z 2015-05-08T01:10:33Z Artificial. This part is ribosome-binding site (RBS) with cis-repressor (cr) sequence. The cis-repressor sequence is placed between the transcription start site and the RBS, and its complementarity with the RBS causes a stem-loop structure to form upon transcription. This secondary structure prevents binding of the 30S ribosomal subunit to the RBS, which inhibits translation. false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa annotation2384852 1 rbs range2384852 1 1 52 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1461004 1 BBa_K1461004 ecf20 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Fluorescens. This part is from Pseudomonas Fluorescens sigma 70 family. ecf20 is a sigma factor which activates ecf20 promoter (BBa_K1461001). false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1461214 1 BBa_K1461214 constitutive cr-ecf20 generator 2014-09-25T11:00:00Z 2015-05-08T01:10:33Z Assembly. The transcription of this generator is induced by arabinose, and the translation of ecf20 is activated by taRNA (BBa_K1461003). false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2401135 1 BBa_K1461203 component2401124 1 BBa_J23101 annotation2401124 1 BBa_J23101 range2401124 1 1 35 annotation2401135 1 BBa_K1461203 range2401135 1 44 820 BBa_K1461203 1 BBa_K1461203 cr-ecf20 (+RBS, +d.term) 2014-09-24T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of crRBS (BBa_K1461000), ecf20 (BBa_K61004), double terminator (BBa_B0015). With promoter, mRNA which codes ecf20 will be transcripted, but not usually translated. taRNA (BBa_K1461003) activates the translation. false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2386542 1 BBa_K1461000 component2386543 1 BBa_K1461004 component2386550 1 BBa_B0015 annotation2386542 1 BBa_K1461000 range2386542 1 1 52 annotation2386543 1 BBa_K1461004 range2386543 1 59 640 annotation2386550 1 BBa_B0015 range2386550 1 649 777 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K1461000_sequence 1 gaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1461203_sequence 1 gaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461004_sequence 1 atgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaa BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461214_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagaggaactctaccattcacctcttggatttgggtattaaagaggagaaaggtacctactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z