LOCUS       BBa_K1461218             958 bp    DNA     linear       30-Dec-2025
DEFINITION  constitutive cr-GFP generator
ACCESSION   BBa_K1461218
VERSION     BBa_K1461218.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..95
                     /label=rbs
     RBS             44..95
                     /label=BBa_K1461000
     CDS             102..821
                     /label=GFP protein
     CDS             102..821
                     /label=BBa_E0040
     stem_loop       841..884
                     /label=stem_loop
     misc_feature    830..909
                     /label=BBa_B0010
     terminator      830..909
                     /label=BBa_B0010
     stem_loop       925..944
                     /label=T7 TE
     misc_feature    951..951
                     /label=stop
     polyA_site      945..958
                     /label=polya
     misc_feature    918..958
                     /label=BBa_B0012
     terminator      918..958
                     /label=BBa_B0012
     terminator      830..958
                     /label=BBa_B0015
     misc_feature    44..958
                     /label=BBa_K1461205
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gaggaactct accattcacc
       61 tcttggattt gggtattaaa gaggagaaag gtacctacta gatgcgtaaa ggagaagaac
      121 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      181 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      241 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
      301 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
      361 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
      421 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
      481 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
      541 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
      601 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
      661 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
      721 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
      781 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
      841 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      901 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//