LOCUS       BBa_K1461221             825 bp    DNA     linear       22-Nov-2024
DEFINITION  ecf20 generator (positive feedback)
ACCESSION   BBa_K1461221
VERSION     BBa_K1461221.1  
FEATURES             Location/Qualifiers
     promoter        1..80
                     /label=BBa_K1461001
     misc_feature    93..96
                     /label=conserved
     RBS             89..100
                     /label=BBa_B0034
     CDS             107..688
                     /label=BBa_K1461004
     stem_loop       708..751
                     /label=stem_loop
     misc_feature    697..776
                     /label=BBa_B0010
     terminator      697..776
                     /label=BBa_B0010
     stem_loop       792..811
                     /label=T7 TE
     misc_feature    818..818
                     /label=stop
     polyA_site      812..825
                     /label=polya
     misc_feature    785..825
                     /label=BBa_B0012
     terminator      785..825
                     /label=BBa_B0012
     terminator      697..825
                     /label=BBa_B0015
ORIGIN
        1 gcgcggataa aaatttcatt tgcccgcgac ggattccccg cccatctatc gttgaaccca
       61 tcagctgcgt tcatcagcga tactagagaa agaggagaaa tactagatga acgaaaccga
      121 tccggatctg gaactgctga aacgtattgg caacaacgat gcacaggcgg tgaaagaaat
      181 ggtgacccgc aaactgccgc gcctgctggc gctggcgagc cgtctgctgg gcgatgcgga
      241 tgaagcgcgc gatattgcgc aggaaagctt tctgcgcatt tggaaacagg cagcgagctg
      301 gcgcagcgaa caggcgcgct ttgatacctg gctgcatcgc gtggcgctga acctgtgcta
      361 tgatcgcctg cgccgtcgta aagaacatgt gccggtggat agcgaacatg cgtgcgaagc
      421 gctggatacc cgcccggcgc cggatgaaca gctggaagcg agcgcgcaga gccgtcgcat
      481 ggcgcaggca ctggatcagc tgccggatcg tcagcgcgaa gcgattgtgc tgcaatatta
      541 tcaggaactg agcaacaccg aagcagcggc actgatgcag attagcgtgg aagcgctgga
      601 aagcctgctg agccgcgcac gtcgcaacct gcgcagccat ctggcggaag cgccgggtgc
      661 ggatctgagc ggccgtcgca aaccgtaata ctagagccag gcatcaaata aaacgaaagg
      721 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      781 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//