LOCUS       BBa_K1461222             852 bp    DNA     linear       26-Nov-2024
DEFINITION  ecf11 generator (positive feedback)
ACCESSION   BBa_K1461222
VERSION     BBa_K1461222.1  
FEATURES             Location/Qualifiers
     promoter        1..80
                     /label=BBa_K1461002
     misc_feature    93..96
                     /label=conserved
     RBS             89..100
                     /label=BBa_B0034
     CDS             107..715
                     /label=BBa_K1461005
     stem_loop       735..778
                     /label=stem_loop
     misc_feature    724..803
                     /label=BBa_B0010
     terminator      724..803
                     /label=BBa_B0010
     stem_loop       819..838
                     /label=T7 TE
     misc_feature    845..845
                     /label=stop
     polyA_site      839..852
                     /label=polya
     misc_feature    812..852
                     /label=BBa_B0012
     terminator      812..852
                     /label=BBa_B0012
     terminator      724..852
                     /label=BBa_B0015
ORIGIN
        1 gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg taacacctct
       61 gcatcgcgaa ccaaaaccag tactagagaa agaggagaaa tactagatgc gcattaccgc
      121 gagcctgcgc accttttgcc atctgagcac cccgcatagc gatagcacca ccagccgcct
      181 gtggattgat gaagtgaccg cggtggcgcg ccagcgcgat cgcgatagct ttatgcgcat
      241 ttatgatcat tttgcgccgc gcctgctgcg ctatctgacc ggcctgaacg tgccggaagg
      301 ccaggcggaa gaactggtgc aggaagtgct gctgaaactg tggcataaag cggaaagctt
      361 tgatccgagc aaagcgagcc tgggcacctg gctgtttcgc attgcgcgca acctgtatat
      421 tgatagcgtg cgcaaagatc gcggctgggt gcaggtgcag aacagcctgg aacagctgga
      481 acgcctggaa gcgccggtgg atcgcaccct ggattatagc cagcgccagg aacagcagct
      541 gaacagcgcg attcagaacc tgccgaccga tcaggcgaaa gtgctgcgca tgagctattt
      601 tgaagcgctg agccatcgcg aaattagcga acgcctggat atgccgctgg gcaccgtgaa
      661 aagctgcctg cgcctggcgt ttcagaaact gcgcagccgc attgaagaaa gctaatacta
      721 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      781 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      841 tctgcgttta ta
//