LOCUS       BBa_K1461223             795 bp    DNA     linear       27-Dec-2024
DEFINITION  anti ecf20 generator (pLac)
ACCESSION   BBa_K1461223
VERSION     BBa_K1461223.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..658
                     /label=BBa_K1461006
     stem_loop       678..721
                     /label=stem_loop
     misc_feature    667..746
                     /label=BBa_B0010
     terminator      667..746
                     /label=BBa_B0010
     stem_loop       762..781
                     /label=T7 TE
     misc_feature    788..788
                     /label=stop
     polyA_site      782..795
                     /label=polya
     misc_feature    755..795
                     /label=BBa_B0012
     terminator      755..795
                     /label=BBa_B0012
     terminator      667..795
                     /label=BBa_B0015
     misc_feature    209..795
                     /label=BBa_K1461206
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ccccggaacg
      241 ttttgtgcat ctggcggatg cgtatggtgc agatctgcaa cgttggccga gcgcagaacg
      301 tgcagcagca caggcactgc tggattgcgg cgatgcgcag gcagtggcag cgctgcgtca
      361 ggcacattgg ctggatagcc agctggatcg ttatcaggtg ccggcaccga gcccggcact
      421 ggcacagcgt attattgcgg cagcacaaca gccgggtgca ccgttttgga gccgctatgc
      481 aggttggctg gcaagcctgg gttgggtggg tgtgggcctg acaggcgtgg cagcgggtat
      541 gctggcggtg gcactgagcc tgccattaag caccagcgca gaagcactgc caagcgtgtt
      601 tgatcagagc gatgcagaat ttgtgctgag cattaacgcg gaagaagcgg aacagtaata
      661 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      721 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      781 ctttctgcgt ttata
//