LOCUS       BBa_K1461224            1026 bp    DNA     linear       30-Dec-2025
DEFINITION  anti ecf11 generator (pLac)
ACCESSION   BBa_K1461224
VERSION     BBa_K1461224.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..889
                     /label=BBa_K1461007
     stem_loop       909..952
                     /label=stem_loop
     misc_feature    898..977
                     /label=BBa_B0010
     terminator      898..977
                     /label=BBa_B0010
     stem_loop       993..1012
                     /label=T7 TE
     misc_feature    1019..1019
                     /label=stop
     polyA_site      1013..1026
                     /label=polya
     misc_feature    986..1026
                     /label=BBa_B0012
     terminator      986..1026
                     /label=BBa_B0012
     terminator      898..1026
                     /label=BBa_B0015
     misc_feature    209..1026
                     /label=BBa_K1461207
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg gcccgctgca
      241 tcatccggat gatgcgaccc tgattagcta tgcggcaggc gcactgagcc aggtgattgc
      301 ggtggtgacc gcagcgcatc tggaacgttg cgcagaatgc cgtgcacgtc tgcgtcaggc
      361 agaacagatt ggtggcgtgc tgatgcagca gagcattagc cgtgtggtgc cgctgaaaag
      421 ccgtatggcg atgctggcac gcctggatga acaggaaacc agcgtggata gcgtgatgca
      481 tgcgatgccg gcagcgaacc atgatccgga tctgctgccg cattgcatgc atgcgcattt
      541 tggccgccat ctgagcaccc tgaaatggaa aaccctgatt ccgggcgtgc agcgcgtgag
      601 cgcacagggc attgaacagg gcaacctgat gctgctgaaa attgcaccag gcgtgagcat
      661 gccggtgcat agccatgaaa gcggtgaaat gaccatggtg ctgaaaggcg cgtatcatga
      721 tgtgcagggt gaatttggcc tgaacgatgt ggcagatctg gatagccata ttcagcatca
      781 gccgattgcg tatccggatc gcgaatgcat ttgcgtgctg gcggcagaaa gcaaactgcg
      841 ttttcatggc tggatggcac gtatgatgca gccgtttttt ggcatttaat actagagcca
      901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      961 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1021 tttata
//