LOCUS       BBa_K1461225            1846 bp    DNA     linear       27-Dec-2024
DEFINITION  ecf20 promoter measurement system
ACCESSION   BBa_K1461225
VERSION     BBa_K1461225.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..738
                     /label=BBa_K1461004
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     terminator      747..875
                     /label=BBa_B0015
     misc_feature    139..875
                     /label=BBa_K1461201
     misc_feature    1..875
                     /label=BBa_K1461209
     promoter        884..963
                     /label=BBa_K1461001
     misc_feature    976..979
                     /label=conserved
     RBS             972..983
                     /label=BBa_B0034
     CDS             990..1709
                     /label=GFP protein
     CDS             990..1709
                     /label=BBa_E0040
     stem_loop       1729..1772
                     /label=stem_loop
     misc_feature    1718..1797
                     /label=BBa_B0010
     terminator      1718..1797
                     /label=BBa_B0010
     stem_loop       1813..1832
                     /label=T7 TE
     misc_feature    1839..1839
                     /label=stop
     polyA_site      1833..1846
                     /label=polya
     misc_feature    1806..1846
                     /label=BBa_B0012
     terminator      1806..1846
                     /label=BBa_B0012
     terminator      1718..1846
                     /label=BBa_B0015
     misc_feature    884..1846
                     /label=BBa_K1461219
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga acgaaaccga tccggatctg
      181 gaactgctga aacgtattgg caacaacgat gcacaggcgg tgaaagaaat ggtgacccgc
      241 aaactgccgc gcctgctggc gctggcgagc cgtctgctgg gcgatgcgga tgaagcgcgc
      301 gatattgcgc aggaaagctt tctgcgcatt tggaaacagg cagcgagctg gcgcagcgaa
      361 caggcgcgct ttgatacctg gctgcatcgc gtggcgctga acctgtgcta tgatcgcctg
      421 cgccgtcgta aagaacatgt gccggtggat agcgaacatg cgtgcgaagc gctggatacc
      481 cgcccggcgc cggatgaaca gctggaagcg agcgcgcaga gccgtcgcat ggcgcaggca
      541 ctggatcagc tgccggatcg tcagcgcgaa gcgattgtgc tgcaatatta tcaggaactg
      601 agcaacaccg aagcagcggc actgatgcag attagcgtgg aagcgctgga aagcctgctg
      661 agccgcgcac gtcgcaacct gcgcagccat ctggcggaag cgccgggtgc ggatctgagc
      721 ggccgtcgca aaccgtaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggcgcgga taaaaatttc
      901 atttgcccgc gacggattcc ccgcccatct atcgttgaac ccatcagctg cgttcatcag
      961 cgatactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag
     1021 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     1081 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     1141 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
     1201 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     1261 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     1321 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     1381 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     1441 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     1501 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     1561 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     1621 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     1681 atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag
     1741 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1801 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//