BBa_K1461225 1 BBa_K1461225 ecf20 promoter measurement system 2014-09-28T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part can be used to characterize the combination of ecf20 (BBa_K1461004) and ecf20 promoter (BBa_K1461001), using GFP as reporter gene. false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2401441 1 BBa_K1461219 component2401428 1 BBa_K1461209 annotation2401428 1 BBa_K1461209 range2401428 1 1 875 annotation2401441 1 BBa_K1461219 range2401441 1 884 1846 BBa_K1461201 1 BBa_K1461201 ecf20 (+RBS, +d.term) 2014-09-24T11:00:00Z 2015-05-08T01:10:33Z Assembly. This part is a composite part of RBS (BBa_B0034), ecf20 (BBa_K1461004), and double terminator (BBa_B0015). false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2386269 1 BBa_K1461004 component2386268 1 BBa_B0034 component2386276 1 BBa_B0015 annotation2386269 1 BBa_K1461004 range2386269 1 19 600 annotation2386276 1 BBa_B0015 range2386276 1 609 737 annotation2386268 1 BBa_B0034 range2386268 1 1 12 BBa_K1461209 1 BBa_K1461209 arabinose-induced ecf20 generator 2014-09-25T11:00:00Z 2015-05-08T01:10:33Z Assembly. This generator codes ecf20, a sigma factor, constitutively. false false _1840_ 0 18036 9 It's complicated false None. false Takefumi Yoshikawa component2401396 1 BBa_K1461201 component2401385 1 BBa_I13453 annotation2401396 1 BBa_K1461201 range2401396 1 139 875 annotation2401385 1 BBa_I13453 range2401385 1 1 130 BBa_I13453 1 BBa_I13453 Pbad promoter 2005-05-24T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PBad promoter from I0500 without AraC. false false _11_ 0 253 6 In stock false true jkm BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1461004 1 BBa_K1461004 ecf20 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Fluorescens. This part is from Pseudomonas Fluorescens sigma 70 family. ecf20 is a sigma factor which activates ecf20 promoter (BBa_K1461001). false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_K1461001 1 BBa_K1461001 ecf20 promoter 2014-09-23T11:00:00Z 2015-05-08T01:10:33Z Pseudomonas Fluorescens. This part is from Pseudomonas Fluorescens sigma 70 family. ecf20 (BBa_K1461004) activates the expression of the downstream genes of this promoter. false false _1840_ 0 18036 9 In stock true None. false Takefumi Yoshikawa BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K1461219 1 BBa_K1461219 ecf20-induced GFP generator 2014-09-26T11:00:00Z 2015-05-08T01:10:33Z Assembly. This generator codes GFP (BBa_E0040), induced by ecf20 (BBa_K1461004). false false _1840_ 0 18036 9 In stock false None. false Takefumi Yoshikawa component2387387 1 BBa_E0040 component2387385 1 BBa_B0034 component2387394 1 BBa_B0015 component2387383 1 BBa_K1461001 annotation2387387 1 BBa_E0040 range2387387 1 107 826 annotation2387394 1 BBa_B0015 range2387394 1 835 963 annotation2387383 1 BBa_K1461001 range2387383 1 1 80 annotation2387385 1 BBa_B0034 range2387385 1 89 100 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1461219_sequence 1 gcgcggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagctgcgttcatcagcgatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I13453_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1461201_sequence 1 aaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461225_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcgcggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagctgcgttcatcagcgatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461209_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1461004_sequence 1 atgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1461001_sequence 1 gcgcggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagctgcgttcatcagcga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z