LOCUS BBa_K1461227 2671 bp DNA linear 27-Dec-2024 DEFINITION resettable single counter system using ecf20 ACCESSION BBa_K1461227 VERSION BBa_K1461227.1 FEATURES Location/Qualifiers stem_loop 758..801 /label=stem_loop misc_feature 747..826 /label=BBa_B0010 terminator 747..826 /label=BBa_B0010 stem_loop 842..861 /label=T7 TE misc_feature 868..868 /label=stop polyA_site 862..875 /label=polya misc_feature 835..875 /label=BBa_B0012 terminator 835..875 /label=BBa_B0012 terminator 747..875 /label=BBa_B0015 promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 CDS 157..738 /label=BBa_K1461004 stem_loop 758..801 /label=stem_loop misc_feature 747..826 /label=BBa_B0010 terminator 747..826 /label=BBa_B0010 stem_loop 842..861 /label=T7 TE misc_feature 868..868 /label=stop polyA_site 862..875 /label=polya misc_feature 835..875 /label=BBa_B0012 terminator 835..875 /label=BBa_B0012 terminator 747..875 /label=BBa_B0015 misc_feature 139..875 /label=BBa_K1461201 misc_feature 1..875 /label=BBa_K1461209 promoter 884..963 /label=BBa_K1461001 misc_feature 976..979 /label=conserved RBS 972..983 /label=BBa_B0034 CDS 990..1731 /label=BBa_K1461008 stem_loop 1751..1794 /label=stem_loop misc_feature 1740..1819 /label=BBa_B0010 terminator 1740..1819 /label=BBa_B0010 stem_loop 1835..1854 /label=T7 TE misc_feature 1861..1861 /label=stop polyA_site 1855..1868 /label=polya misc_feature 1828..1868 /label=BBa_B0012 terminator 1828..1868 /label=BBa_B0012 terminator 1740..1868 /label=BBa_B0015 stem_loop 2554..2597 /label=stem_loop misc_feature 2543..2622 /label=BBa_B0010 terminator 2543..2622 /label=BBa_B0010 stem_loop 2638..2657 /label=T7 TE misc_feature 2664..2664 /label=stop polyA_site 2658..2671 /label=polya misc_feature 2631..2671 /label=BBa_B0012 terminator 2631..2671 /label=BBa_B0012 terminator 2543..2671 /label=BBa_B0015 CDS 1877..1964 /label=end of LacI coding region (inactive) misc_feature 1877..2076 /label=BBa_R0010 misc_feature 1965..2002 /label=CAP binding site promoter 2013..2018 /label=-35 promoter 2037..2042 /label=-10 misc_feature 2042..2076 /label=LacI binding site misc_feature 2049..2049 /label=start promoter 1877..2076 /label=BBa_R0010 misc_feature 2089..2092 /label=conserved RBS 2085..2096 /label=BBa_B0034 CDS 2103..2534 /label=BBa_K1461006 stem_loop 2554..2597 /label=stem_loop misc_feature 2543..2622 /label=BBa_B0010 terminator 2543..2622 /label=BBa_B0010 stem_loop 2638..2657 /label=T7 TE misc_feature 2664..2664 /label=stop polyA_site 2658..2671 /label=polya misc_feature 2631..2671 /label=BBa_B0012 terminator 2631..2671 /label=BBa_B0012 terminator 2543..2671 /label=BBa_B0015 misc_feature 2085..2671 /label=BBa_K1461206 misc_feature 1877..2671 /label=BBa_K1461223 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga acgaaaccga tccggatctg 181 gaactgctga aacgtattgg caacaacgat gcacaggcgg tgaaagaaat ggtgacccgc 241 aaactgccgc gcctgctggc gctggcgagc cgtctgctgg gcgatgcgga tgaagcgcgc 301 gatattgcgc aggaaagctt tctgcgcatt tggaaacagg cagcgagctg gcgcagcgaa 361 caggcgcgct ttgatacctg gctgcatcgc gtggcgctga acctgtgcta tgatcgcctg 421 cgccgtcgta aagaacatgt gccggtggat agcgaacatg cgtgcgaagc gctggatacc 481 cgcccggcgc cggatgaaca gctggaagcg agcgcgcaga gccgtcgcat ggcgcaggca 541 ctggatcagc tgccggatcg tcagcgcgaa gcgattgtgc tgcaatatta tcaggaactg 601 agcaacaccg aagcagcggc actgatgcag attagcgtgg aagcgctgga aagcctgctg 661 agccgcgcac gtcgcaacct gcgcagccat ctggcggaag cgccgggtgc ggatctgagc 721 ggccgtcgca aaccgtaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggcgcgga taaaaatttc 901 atttgcccgc gacggattcc ccgcccatct atcgttgaac ccatcagctg cgttcatcag 961 cgatactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag 1021 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg 1081 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg 1141 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct 1201 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag 1261 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg 1321 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta 1381 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat 1441 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca 1501 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg 1561 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc 1621 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac 1681 atggcatgga tgaactatac aaacgacgaa aactacgctg ctgtctaata atactagagc 1741 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1801 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1861 cgtttatata ctagagcaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 1921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 1981 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 2041 ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag gagaaatact 2101 agatgacccc ggaacgtttt gtgcatctgg cggatgcgta tggtgcagat ctgcaacgtt 2161 ggccgagcgc agaacgtgca gcagcacagg cactgctgga ttgcggcgat gcgcaggcag 2221 tggcagcgct gcgtcaggca cattggctgg atagccagct ggatcgttat caggtgccgg 2281 caccgagccc ggcactggca cagcgtatta ttgcggcagc acaacagccg ggtgcaccgt 2341 tttggagccg ctatgcaggt tggctggcaa gcctgggttg ggtgggtgtg ggcctgacag 2401 gcgtggcagc gggtatgctg gcggtggcac tgagcctgcc attaagcacc agcgcagaag 2461 cactgccaag cgtgtttgat cagagcgatg cagaatttgt gctgagcatt aacgcggaag 2521 aagcggaaca gtaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 2581 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 2641 tcaccttcgg gtgggccttt ctgcgtttat a //