##gff-version 3
##sequence-region BBa_K1461227 1 2671
BBa_K1461227	.	terminator	2543	2671	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461227	.	terminator	2631	2671	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461227	.	engineered_region	2631	2671	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461227	.	polyA_site	2658	2671	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461227	.	stop_codon	2664	2664	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461227	.	stem_loop	2638	2657	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461227	.	terminator	2543	2622	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461227	.	engineered_region	2543	2622	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461227	.	stem_loop	2554	2597	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461227	.	terminator	1740	1868	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461227	.	terminator	1828	1868	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461227	.	engineered_region	1828	1868	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461227	.	polyA_site	1855	1868	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461227	.	stop_codon	1861	1861	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461227	.	stem_loop	1835	1854	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461227	.	terminator	1740	1819	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461227	.	engineered_region	1740	1819	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461227	.	stem_loop	1751	1794	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461227	.	terminator	747	875	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1461227	.	terminator	835	875	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461227	.	engineered_region	835	875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461227	.	polyA_site	862	875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461227	.	stop_codon	868	868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461227	.	stem_loop	842	861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461227	.	terminator	747	826	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461227	.	engineered_region	747	826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461227	.	stem_loop	758	801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461227	.	ribosome_entry_site	972	983	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1461227	.	conserved_region	976	979	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461227	.	CDS	990	1731	.	+	0	ID=BBa_K1461008;Name=BBa_K1461008
BBa_K1461227	.	engineered_region	1877	2671	.	+	0	ID=BBa_K1461223;Name=BBa_K1461223
BBa_K1461227	.	engineered_region	2085	2671	.	+	0	ID=BBa_K1461206;Name=BBa_K1461206;Parent=BBa_K1461223
BBa_K1461227	.	ribosome_entry_site	2085	2096	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461206
BBa_K1461227	.	conserved_region	2089	2092	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461227	.	CDS	2103	2534	.	+	0	ID=BBa_K1461006;Name=BBa_K1461006;Parent=BBa_K1461206
BBa_K1461227	.	terminator	2543	2671	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461206
BBa_K1461227	.	terminator	2631	2671	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461227	.	engineered_region	2631	2671	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461227	.	polyA_site	2658	2671	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461227	.	stop_codon	2664	2664	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461227	.	stem_loop	2638	2657	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461227	.	terminator	2543	2622	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461227	.	engineered_region	2543	2622	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461227	.	stem_loop	2554	2597	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461227	.	promoter	1877	2076	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1461223
BBa_K1461227	.	non_covalent_binding_site	1965	2002	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1461227	.	start_codon	2049	2049	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1461227	.	engineered_region	1877	2076	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1461227	.	promoter	2037	2042	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1461227	.	non_covalent_binding_site	2042	2076	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1461227	.	CDS	1877	1964	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1461227	.	promoter	2013	2018	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1461227	.	promoter	884	963	.	+	0	ID=BBa_K1461001;Name=BBa_K1461001
BBa_K1461227	.	engineered_region	1	875	.	+	0	ID=BBa_K1461209;Name=BBa_K1461209
BBa_K1461227	.	engineered_region	139	875	.	+	0	ID=BBa_K1461201;Name=BBa_K1461201;Parent=BBa_K1461209
BBa_K1461227	.	terminator	747	875	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1461201
BBa_K1461227	.	terminator	835	875	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1461227	.	engineered_region	835	875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1461227	.	polyA_site	862	875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1461227	.	stop_codon	868	868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1461227	.	stem_loop	842	861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1461227	.	terminator	747	826	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1461227	.	engineered_region	747	826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1461227	.	stem_loop	758	801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1461227	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1461201
BBa_K1461227	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1461227	.	CDS	157	738	.	+	0	ID=BBa_K1461004;Name=BBa_K1461004;Parent=BBa_K1461201
BBa_K1461227	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K1461209
>BBa_K1461227
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacc
cgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaa
gctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggc
gctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctg
gatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgc
cggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagat
tagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcg
gatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagaggcgcggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagc
tgcgttcatcagcgatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccc
aattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatac
ggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtt
atggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtta
tgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacc
cttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaact
ataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacat
tgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagac
aaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttg
taacagctgctgggattacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtctaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctc
cccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa
ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgt
gagcggataacaatttcacacatactagagaaagaggagaaatactagatgaccccggaacgttttgtgcatctggcgg
atgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgc
gcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccg
gcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaa
gcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcac
cagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaa
cagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata