BBa_I13453
1
BBa_I13453
Pbad promoter
2005-05-24T11:00:00Z
2015-08-31T04:07:34Z
Released HQ 2013
PBad promoter from I0500 without AraC.
false
false
_11_
0
253
6
In stock
false
true
jkm
BBa_K1461004
1
BBa_K1461004
ecf20
2014-09-23T11:00:00Z
2015-05-08T01:10:33Z
Pseudomonas Fluorescens.
This part is from Pseudomonas Fluorescens sigma 70 family. ecf20 is a sigma factor which activates ecf20 promoter (BBa_K1461001).
false
false
_1840_
0
18036
9
In stock
true
None.
false
Takefumi Yoshikawa
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K1461006
1
BBa_K1461006
anti ecf20
2014-09-23T11:00:00Z
2015-05-08T01:10:33Z
Pseudomonas Fluorescens.
This part is an anti-sigma factor. Anti ecf20 prevents ecf20 from activating ecf20 promoter.
false
false
_1840_
0
18036
9
Not in stock
true
None.
false
Takefumi Yoshikawa
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961225
1
-10
range1961225
1
161
166
annotation1961224
1
-35
range1961224
1
137
142
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961227
1
start
range1961227
1
173
173
annotation1961226
1
LacI binding site
range1961226
1
166
200
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K1461209
1
BBa_K1461209
arabinose-induced ecf20 generator
2014-09-25T11:00:00Z
2015-05-08T01:10:33Z
Assembly.
This generator codes ecf20, a sigma factor, constitutively.
false
false
_1840_
0
18036
9
It's complicated
false
None.
false
Takefumi Yoshikawa
component2401385
1
BBa_I13453
component2401396
1
BBa_K1461201
annotation2401396
1
BBa_K1461201
range2401396
1
139
875
annotation2401385
1
BBa_I13453
range2401385
1
1
130
BBa_K1461227
1
BBa_K1461227
resettable single counter system using ecf20
2014-09-28T11:00:00Z
2015-05-08T01:10:33Z
Assembly.
This part can be used to characterize the reset-function of our σ-Re Counter project. Arabinose-induction causes GFP expression, and IPTG-induction extinguishes the GFP expression.
false
false
_1840_
0
18036
9
It's complicated
false
None.
false
Takefumi Yoshikawa
component2418406
1
BBa_K1461008
component2418395
1
BBa_B0015
component2418432
1
BBa_K1461223
component2418426
1
BBa_B0015
component2418413
1
BBa_B0015
component2418402
1
BBa_K1461209
component2418403
1
BBa_K1461001
component2418405
1
BBa_B0034
annotation2418402
1
BBa_K1461209
range2418402
1
1
875
annotation2418432
1
BBa_K1461223
range2418432
1
1877
2671
annotation2418426
1
BBa_B0015
range2418426
1
2543
2671
annotation2418405
1
BBa_B0034
range2418405
1
972
983
annotation2418406
1
BBa_K1461008
range2418406
1
990
1731
annotation2418403
1
BBa_K1461001
range2418403
1
884
963
annotation2418395
1
BBa_B0015
range2418395
1
747
875
annotation2418413
1
BBa_B0015
range2418413
1
1740
1868
BBa_K1461206
1
BBa_K1461206
anti ecf20 (+RBS, +d.term)
2014-09-25T11:00:00Z
2015-05-08T01:10:33Z
Assembly.
This part is a composite part of RBS (BBa_B0034), anti ecf20 (BBa_K1461006), and double terminator (BBa_B0015).
false
false
_1840_
0
18036
9
It's complicated
false
None.
false
Takefumi Yoshikawa
component2386615
1
BBa_K1461006
component2386614
1
BBa_B0034
component2386622
1
BBa_B0015
annotation2386614
1
BBa_B0034
range2386614
1
1
12
annotation2386615
1
BBa_K1461006
range2386615
1
19
450
annotation2386622
1
BBa_B0015
range2386622
1
459
587
BBa_K1461201
1
BBa_K1461201
ecf20 (+RBS, +d.term)
2014-09-24T11:00:00Z
2015-05-08T01:10:33Z
Assembly.
This part is a composite part of RBS (BBa_B0034), ecf20 (BBa_K1461004), and double terminator (BBa_B0015).
false
false
_1840_
0
18036
9
In stock
false
None.
false
Takefumi Yoshikawa
component2386269
1
BBa_K1461004
component2386268
1
BBa_B0034
component2386276
1
BBa_B0015
annotation2386268
1
BBa_B0034
range2386268
1
1
12
annotation2386269
1
BBa_K1461004
range2386269
1
19
600
annotation2386276
1
BBa_B0015
range2386276
1
609
737
BBa_K1461001
1
BBa_K1461001
ecf20 promoter
2014-09-23T11:00:00Z
2015-05-08T01:10:33Z
Pseudomonas Fluorescens.
This part is from Pseudomonas Fluorescens sigma 70 family. ecf20 (BBa_K1461004) activates the expression of the downstream genes of this promoter.
false
false
_1840_
0
18036
9
In stock
true
None.
false
Takefumi Yoshikawa
BBa_K1461223
1
BBa_K1461223
anti ecf20 generator (pLac)
2014-09-26T11:00:00Z
2015-05-08T01:10:33Z
Assembly.
This generator codes anti ecf20 (BBa_K1461~~), which prevents ecf20 (BBa_K1461~~~) from activating the expression of ecf20 promoter (BBa_K1461~~). This part works as a reset factor.
false
false
_1840_
0
18036
9
It's complicated
false
None.
false
Takefumi Yoshikawa
component2387503
1
BBa_K1461206
component2387486
1
BBa_R0010
annotation2387503
1
BBa_K1461206
range2387503
1
209
795
annotation2387486
1
BBa_R0010
range2387486
1
1
200
BBa_K1461008
1
BBa_K1461008
GFP (AAV tag +)
2014-10-12T11:00:00Z
2015-05-08T01:10:33Z
PCR.
This part is GFP(mut3b) (BBa_E0040) with an AAV degradation tag added by PCR.
false
false
_1840_
0
18036
9
Not in stock
false
None.
false
Takefumi Yoshikawa
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I13453_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1461201_sequence
1
aaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1461209_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1461001_sequence
1
gcgcggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagctgcgttcatcagcga
BBa_K1461206_sequence
1
aaagaggagaaatactagatgaccccggaacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1461006_sequence
1
atgaccccggaacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaa
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1461223_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaccccggaacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1461227_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcgcggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagctgcgttcatcagcgatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaccccggaacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1461008_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtctaataa
BBa_K1461004_sequence
1
atgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z