BBa_K1461227
type: ComponentDefinition
persistent identity: BBa K1461227
display id: BBa_K1461227
version: 1
was generated by: iGEM to SBOL conversion
description: resettable single counter system using ecf20
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Assembly.
owned by: james
owned by: myers
mutable description: This part can be used to characterize the reset-function of our σ-Re Counter project. Arabinose-induction causes GFP expression, and IPTG-induction extinguishes the GFP expression.
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_K1461209
component: BBa_B0015
component: BBa_K1461223
component: BBa_B0034
component: BBa_K1461001
component: BBa_K1461008
sequence annotation: BBa_K1461209
sequence annotation: BBa_B0015
sequence annotation: BBa_K1461001
sequence annotation: BBa_B0034
sequence annotation: BBa_K1461008
sequence annotation: BBa_K1461223
sequence: https://synbiohub.org/public/igem/BBa_K1461227_sequence/1

BBa_K1461209
type: Component
persistent identity: component2418402
display id: component2418402
version: 1
top level: BBa_K1461227
definition: BBa_K1461209
access: public

BBa_B0015
type: Component
persistent identity: component2418395
display id: component2418395
version: 1
top level: BBa_K1461227
definition: BBa_B0015
access: public

BBa_K1461223
type: Component
persistent identity: component2418432
display id: component2418432
version: 1
top level: BBa_K1461227
definition: BBa_K1461223
access: public

BBa_B0015
type: Component
persistent identity: component2418426
display id: component2418426
version: 1
top level: BBa_K1461227
definition: BBa_B0015
access: public

BBa_B0034
type: Component
persistent identity: component2418405
display id: component2418405
version: 1
top level: BBa_K1461227
definition: BBa_B0034
access: public

BBa_K1461001
type: Component
persistent identity: component2418403
display id: component2418403
version: 1
top level: BBa_K1461227
definition: BBa_K1461001
access: public

BBa_B0015
type: Component
persistent identity: component2418413
display id: component2418413
version: 1
top level: BBa_K1461227
definition: BBa_B0015
access: public

BBa_K1461008
type: Component
persistent identity: component2418406
display id: component2418406
version: 1
top level: BBa_K1461227
definition: BBa_K1461008
access: public

BBa_K1461209
type: SequenceAnnotation
persistent identity: annotation2418402
display id: annotation2418402
version: 1
top level: BBa_K1461227
location: https://synbiohub.org/public/igem/BBa_K1461227/annotation2418402/range2418402/1
component: BBa_K1461209

https://synbiohub.org/public/igem/BBa_K1461227/annotation2418402/range2418402/1
type: Range
persistent identity: range2418402
display id: range2418402
version: 1
top level: BBa_K1461227
direction: direction/reverse
start: 1
end: 875
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2418395
display id: annotation2418395
version: 1
top level: BBa_K1461227
location: https://synbiohub.org/public/igem/BBa_K1461227/annotation2418395/range2418395/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461227/annotation2418395/range2418395/1
type: Range
persistent identity: range2418395
display id: range2418395
version: 1
top level: BBa_K1461227
direction: direction/reverse
start: 747
end: 875
orientation: inline

BBa_K1461001
type: SequenceAnnotation
persistent identity: annotation2418403
display id: annotation2418403
version: 1
top level: BBa_K1461227
location: https://synbiohub.org/public/igem/BBa_K1461227/annotation2418403/range2418403/1
component: BBa_K1461001

https://synbiohub.org/public/igem/BBa_K1461227/annotation2418403/range2418403/1
type: Range
persistent identity: range2418403
display id: range2418403
version: 1
top level: BBa_K1461227
direction: direction/reverse
start: 884
end: 963
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418405
display id: annotation2418405
version: 1
top level: BBa_K1461227
location: https://synbiohub.org/public/igem/BBa_K1461227/annotation2418405/range2418405/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1461227/annotation2418405/range2418405/1
type: Range
persistent identity: range2418405
display id: range2418405
version: 1
top level: BBa_K1461227
direction: direction/reverse
start: 972
end: 983
orientation: inline

BBa_K1461008
type: SequenceAnnotation
persistent identity: annotation2418406
display id: annotation2418406
version: 1
top level: BBa_K1461227
location: https://synbiohub.org/public/igem/BBa_K1461227/annotation2418406/range2418406/1
component: BBa_K1461008

https://synbiohub.org/public/igem/BBa_K1461227/annotation2418406/range2418406/1
type: Range
persistent identity: range2418406
display id: range2418406
version: 1
top level: BBa_K1461227
direction: direction/reverse
start: 990
end: 1731
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2418413
display id: annotation2418413
version: 1
top level: BBa_K1461227
location: https://synbiohub.org/public/igem/BBa_K1461227/annotation2418413/range2418413/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461227/annotation2418413/range2418413/1
type: Range
persistent identity: range2418413
display id: range2418413
version: 1
top level: BBa_K1461227
direction: direction/reverse
start: 1740
end: 1868
orientation: inline

BBa_K1461223
type: SequenceAnnotation
persistent identity: annotation2418432
display id: annotation2418432
version: 1
top level: BBa_K1461227
location: https://synbiohub.org/public/igem/BBa_K1461227/annotation2418432/range2418432/1
component: BBa_K1461223

https://synbiohub.org/public/igem/BBa_K1461227/annotation2418432/range2418432/1
type: Range
persistent identity: range2418432
display id: range2418432
version: 1
top level: BBa_K1461227
direction: direction/reverse
start: 1877
end: 2671
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2418426
display id: annotation2418426
version: 1
top level: BBa_K1461227
location: https://synbiohub.org/public/igem/BBa_K1461227/annotation2418426/range2418426/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461227/annotation2418426/range2418426/1
type: Range
persistent identity: range2418426
display id: range2418426
version: 1
top level: BBa_K1461227
direction: direction/reverse
start: 2543
end: 2671
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1461001
type: ComponentDefinition
persistent identity: BBa K1461001
display id: BBa_K1461001
version: 1
was generated by: iGEM to SBOL conversion
description: ecf20 promoter
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Pseudomonas Fluorescens.
owned by: james
owned by: myers
mutable description: This part is from Pseudomonas Fluorescens sigma 70 family. ecf20 (BBa_K1461004) activates the expression of the downstream genes of this promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1461001_sequence/1

BBa_K1461209
type: ComponentDefinition
persistent identity: BBa K1461209
display id: BBa_K1461209
version: 1
was generated by: iGEM to SBOL conversion
description: arabinose-induced ecf20 generator
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Assembly.
owned by: james
owned by: myers
mutable description: This generator codes ecf20, a sigma factor, constitutively.
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K1461201
component: BBa_I13453
sequence annotation: BBa_I13453
sequence annotation: BBa_K1461201
sequence: https://synbiohub.org/public/igem/BBa_K1461209_sequence/1

BBa_K1461201
type: Component
persistent identity: component2401396
display id: component2401396
version: 1
top level: BBa_K1461209
definition: BBa_K1461201
access: public

BBa_I13453
type: Component
persistent identity: component2401385
display id: component2401385
version: 1
top level: BBa_K1461209
definition: BBa_I13453
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2401385
display id: annotation2401385
version: 1
top level: BBa_K1461209
location: https://synbiohub.org/public/igem/BBa_K1461209/annotation2401385/range2401385/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1461209/annotation2401385/range2401385/1
type: Range
persistent identity: range2401385
display id: range2401385
version: 1
top level: BBa_K1461209
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_K1461201
type: SequenceAnnotation
persistent identity: annotation2401396
display id: annotation2401396
version: 1
top level: BBa_K1461209
location: https://synbiohub.org/public/igem/BBa_K1461209/annotation2401396/range2401396/1
component: BBa_K1461201

https://synbiohub.org/public/igem/BBa_K1461209/annotation2401396/range2401396/1
type: Range
persistent identity: range2401396
display id: range2401396
version: 1
top level: BBa_K1461209
direction: direction/reverse
start: 139
end: 875
orientation: inline

BBa_K1461223
type: ComponentDefinition
persistent identity: BBa K1461223
display id: BBa_K1461223
version: 1
was generated by: iGEM to SBOL conversion
description: anti ecf20 generator (pLac)
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Assembly.
owned by: james
owned by: myers
mutable description: This generator codes anti ecf20 (BBa_K1461~~), which prevents ecf20 (BBa_K1461~~~) from activating the expression of ecf20 promoter (BBa_K1461~~). This part works as a reset factor.
discontinued: false
dominant: false
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_R0010
component: BBa_K1461206
sequence annotation: BBa_R0010
sequence annotation: BBa_K1461206
sequence: https://synbiohub.org/public/igem/BBa_K1461223_sequence/1

BBa_R0010
type: Component
persistent identity: component2387486
display id: component2387486
version: 1
top level: BBa_K1461223
definition: LacI
access: public

BBa_K1461206
type: Component
persistent identity: component2387503
display id: component2387503
version: 1
top level: BBa_K1461223
definition: BBa_K1461206
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2387486
display id: annotation2387486
version: 1
top level: BBa_K1461223
location: https://synbiohub.org/public/igem/BBa_K1461223/annotation2387486/range2387486/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1461223/annotation2387486/range2387486/1
type: Range
persistent identity: range2387486
display id: range2387486
version: 1
top level: BBa_K1461223
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K1461206
type: SequenceAnnotation
persistent identity: annotation2387503
display id: annotation2387503
version: 1
top level: BBa_K1461223
location: https://synbiohub.org/public/igem/BBa_K1461223/annotation2387503/range2387503/1
component: BBa_K1461206

https://synbiohub.org/public/igem/BBa_K1461223/annotation2387503/range2387503/1
type: Range
persistent identity: range2387503
display id: range2387503
version: 1
top level: BBa_K1461223
direction: direction/reverse
start: 209
end: 795
orientation: inline

BBa_K1461008
type: ComponentDefinition
persistent identity: BBa K1461008
display id: BBa_K1461008
version: 1
was generated by: iGEM to SBOL conversion
description: GFP (AAV tag +)
created: 2014-10-12T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: PCR.
owned by: james
owned by: myers
mutable description: This part is GFP(mut3b) (BBa_E0040) with an AAV degradation tag added by PCR.
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1461008_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K1461201
type: ComponentDefinition
persistent identity: BBa K1461201
display id: BBa_K1461201
version: 1
was generated by: iGEM to SBOL conversion
description: ecf20 (+RBS, +d.term)
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Assembly.
owned by: james
owned by: myers
mutable description: This part is a composite part of RBS (BBa_B0034), ecf20 (BBa_K1461004), and double terminator (BBa_B0015).
discontinued: false
dominant: false
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0015
component: BBa_K1461004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1461004
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1461201_sequence/1

BBa_B0034
type: Component
persistent identity: component2386268
display id: component2386268
version: 1
top level: BBa_K1461201
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2386276
display id: component2386276
version: 1
top level: BBa_K1461201
definition: BBa_B0015
access: public

BBa_K1461004
type: Component
persistent identity: component2386269
display id: component2386269
version: 1
top level: BBa_K1461201
definition: BBa_K1461004
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386268
display id: annotation2386268
version: 1
top level: BBa_K1461201
location: https://synbiohub.org/public/igem/BBa_K1461201/annotation2386268/range2386268/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1461201/annotation2386268/range2386268/1
type: Range
persistent identity: range2386268
display id: range2386268
version: 1
top level: BBa_K1461201
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1461004
type: SequenceAnnotation
persistent identity: annotation2386269
display id: annotation2386269
version: 1
top level: BBa_K1461201
location: https://synbiohub.org/public/igem/BBa_K1461201/annotation2386269/range2386269/1
component: BBa_K1461004

https://synbiohub.org/public/igem/BBa_K1461201/annotation2386269/range2386269/1
type: Range
persistent identity: range2386269
display id: range2386269
version: 1
top level: BBa_K1461201
direction: direction/reverse
start: 19
end: 600
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386276
display id: annotation2386276
version: 1
top level: BBa_K1461201
location: https://synbiohub.org/public/igem/BBa_K1461201/annotation2386276/range2386276/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461201/annotation2386276/range2386276/1
type: Range
persistent identity: range2386276
display id: range2386276
version: 1
top level: BBa_K1461201
direction: direction/reverse
start: 609
end: 737
orientation: inline

BBa_K1461206
type: ComponentDefinition
persistent identity: BBa K1461206
display id: BBa_K1461206
version: 1
was generated by: iGEM to SBOL conversion
description: anti ecf20 (+RBS, +d.term)
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Assembly.
owned by: james
owned by: myers
mutable description: This part is a composite part of RBS (BBa_B0034), anti ecf20 (BBa_K1461006), and double terminator (BBa_B0015).
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1461006
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1461006
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1461206_sequence/1

BBa_B0015
type: Component
persistent identity: component2386622
display id: component2386622
version: 1
top level: BBa_K1461206
definition: BBa_B0015
access: public

BBa_K1461006
type: Component
persistent identity: component2386615
display id: component2386615
version: 1
top level: BBa_K1461206
definition: BBa_K1461006
access: public

BBa_B0034
type: Component
persistent identity: component2386614
display id: component2386614
version: 1
top level: BBa_K1461206
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386614
display id: annotation2386614
version: 1
top level: BBa_K1461206
location: https://synbiohub.org/public/igem/BBa_K1461206/annotation2386614/range2386614/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1461206/annotation2386614/range2386614/1
type: Range
persistent identity: range2386614
display id: range2386614
version: 1
top level: BBa_K1461206
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1461006
type: SequenceAnnotation
persistent identity: annotation2386615
display id: annotation2386615
version: 1
top level: BBa_K1461206
location: https://synbiohub.org/public/igem/BBa_K1461206/annotation2386615/range2386615/1
component: BBa_K1461006

https://synbiohub.org/public/igem/BBa_K1461206/annotation2386615/range2386615/1
type: Range
persistent identity: range2386615
display id: range2386615
version: 1
top level: BBa_K1461206
direction: direction/reverse
start: 19
end: 450
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386622
display id: annotation2386622
version: 1
top level: BBa_K1461206
location: https://synbiohub.org/public/igem/BBa_K1461206/annotation2386622/range2386622/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1461206/annotation2386622/range2386622/1
type: Range
persistent identity: range2386622
display id: range2386622
version: 1
top level: BBa_K1461206
direction: direction/reverse
start: 459
end: 587
orientation: inline

BBa_K1461004
type: ComponentDefinition
persistent identity: BBa K1461004
display id: BBa_K1461004
version: 1
was generated by: iGEM to SBOL conversion
description: ecf20
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Pseudomonas Fluorescens.
owned by: james
owned by: myers
mutable description: This part is from Pseudomonas Fluorescens sigma 70 family. ecf20 is a sigma factor which activates ecf20 promoter (BBa_K1461001).
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1461004_sequence/1

BBa_K1461006
type: ComponentDefinition
persistent identity: BBa K1461006
display id: BBa_K1461006
version: 1
was generated by: iGEM to SBOL conversion
description: anti ecf20
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:33Z
mutable provenance: Pseudomonas Fluorescens.
owned by: james
owned by: myers
mutable description: This part is an anti-sigma factor. Anti ecf20 prevents ecf20 from activating ecf20 promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _1840_
m user id: 0
owner id: 18036
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: None.
star: false
creator: Takefumi Yoshikawa
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1461006_sequence/1

https://synbiohub.org/public/igem/BBa_K1461227_sequence/1
type: Sequence
persistent identity: BBa K1461227 sequence
display id: BBa_K1461227_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcgcggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagctgcgttcatcagcgatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcatta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461001_sequence/1
type: Sequence
persistent identity: BBa K1461001 sequence
display id: BBa_K1461001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgcggataaaaatttcatttgcccgcgacggattccccgcccatctatcgttgaacccatcagctgcgttcatcagcga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461209_sequence/1
type: Sequence
persistent identity: BBa K1461209 sequence
display id: BBa_K1461209_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461223_sequence/1
type: Sequence
persistent identity: BBa K1461223 sequence
display id: BBa_K1461223_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaccccggaacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461008_sequence/1
type: Sequence
persistent identity: BBa K1461008 sequence
display id: BBa_K1461008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacgacgaaaactacgctgctgtctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461201_sequence/1
type: Sequence
persistent identity: BBa K1461201 sequence
display id: BBa_K1461201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461206_sequence/1
type: Sequence
persistent identity: BBa K1461206 sequence
display id: BBa_K1461206_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccccggaacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461004_sequence/1
type: Sequence
persistent identity: BBa K1461004 sequence
display id: BBa_K1461004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacgaaaccgatccggatctggaactgctgaaacgtattggcaacaacgatgcacaggcggtgaaagaaatggtgacccgcaaactgccgcgcctgctggcgctggcgagccgtctgctgggcgatgcggatgaagcgcgcgatattgcgcaggaaagctttctgcgcatttggaaacaggcagcgagctggcgcagcgaacaggcgcgctttgatacctggctgcatcgcgtggcgctgaacctgtgctatgatcgcctgcgccgtcgtaaagaacatgtgccggtggatagcgaacatgcgtgcgaagcgctggatacccgcccggcgccggatgaacagctggaagcgagcgcgcagagccgtcgcatggcgcaggcactggatcagctgccggatcgtcagcgcgaagcgattgtgctgcaatattatcaggaactgagcaacaccgaagcagcggcactgatgcagattagcgtggaagcgctggaaagcctgctgagccgcgcacgtcgcaacctgcgcagccatctggcggaagcgccgggtgcggatctgagcggccgtcgcaaaccgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1461006_sequence/1
type: Sequence
persistent identity: BBa K1461006 sequence
display id: BBa_K1461006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccccggaacgttttgtgcatctggcggatgcgtatggtgcagatctgcaacgttggccgagcgcagaacgtgcagcagcacaggcactgctggattgcggcgatgcgcaggcagtggcagcgctgcgtcaggcacattggctggatagccagctggatcgttatcaggtgccggcaccgagcccggcactggcacagcgtattattgcggcagcacaacagccgggtgcaccgttttggagccgctatgcaggttggctggcaagcctgggttgggtgggtgtgggcctgacaggcgtggcagcgggtatgctggcggtggcactgagcctgccattaagcaccagcgcagaagcactgccaagcgtgtttgatcagagcgatgcagaatttgtgctgagcattaacgcggaagaagcggaacagtaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z