LOCUS BBa_K1461228 2576 bp DNA linear 16-Dec-2024 DEFINITION reset function using ecf20 ACCESSION BBa_K1461228 VERSION BBa_K1461228.1 FEATURES Location/Qualifiers stem_loop 663..706 /label=stem_loop misc_feature 652..731 /label=BBa_B0010 terminator 652..731 /label=BBa_B0010 stem_loop 747..766 /label=T7 TE misc_feature 773..773 /label=stop polyA_site 767..780 /label=polya misc_feature 740..780 /label=BBa_B0012 terminator 740..780 /label=BBa_B0012 terminator 652..780 /label=BBa_B0015 promoter 1..35 /label=BBa_J23101 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..643 /label=BBa_K1461004 stem_loop 663..706 /label=stem_loop misc_feature 652..731 /label=BBa_B0010 terminator 652..731 /label=BBa_B0010 stem_loop 747..766 /label=T7 TE misc_feature 773..773 /label=stop polyA_site 767..780 /label=polya misc_feature 740..780 /label=BBa_B0012 terminator 740..780 /label=BBa_B0012 terminator 652..780 /label=BBa_B0015 misc_feature 44..780 /label=BBa_K1461201 misc_feature 1..780 /label=BBa_K1461210 promoter 789..868 /label=BBa_K1461001 misc_feature 881..884 /label=conserved RBS 877..888 /label=BBa_B0034 CDS 895..1636 /label=BBa_K1461008 stem_loop 1656..1699 /label=stem_loop misc_feature 1645..1724 /label=BBa_B0010 terminator 1645..1724 /label=BBa_B0010 stem_loop 1740..1759 /label=T7 TE misc_feature 1766..1766 /label=stop polyA_site 1760..1773 /label=polya misc_feature 1733..1773 /label=BBa_B0012 terminator 1733..1773 /label=BBa_B0012 terminator 1645..1773 /label=BBa_B0015 stem_loop 2459..2502 /label=stem_loop misc_feature 2448..2527 /label=BBa_B0010 terminator 2448..2527 /label=BBa_B0010 stem_loop 2543..2562 /label=T7 TE misc_feature 2569..2569 /label=stop polyA_site 2563..2576 /label=polya misc_feature 2536..2576 /label=BBa_B0012 terminator 2536..2576 /label=BBa_B0012 terminator 2448..2576 /label=BBa_B0015 CDS 1782..1869 /label=end of LacI coding region (inactive) misc_feature 1782..1981 /label=BBa_R0010 misc_feature 1870..1907 /label=CAP binding site promoter 1918..1923 /label=-35 promoter 1942..1947 /label=-10 misc_feature 1947..1981 /label=LacI binding site misc_feature 1954..1954 /label=start promoter 1782..1981 /label=BBa_R0010 misc_feature 1994..1997 /label=conserved RBS 1990..2001 /label=BBa_B0034 CDS 2008..2439 /label=BBa_K1461006 stem_loop 2459..2502 /label=stem_loop misc_feature 2448..2527 /label=BBa_B0010 terminator 2448..2527 /label=BBa_B0010 stem_loop 2543..2562 /label=T7 TE misc_feature 2569..2569 /label=stop polyA_site 2563..2576 /label=polya misc_feature 2536..2576 /label=BBa_B0012 terminator 2536..2576 /label=BBa_B0012 terminator 2448..2576 /label=BBa_B0015 misc_feature 1990..2576 /label=BBa_K1461206 misc_feature 1782..2576 /label=BBa_K1461223 ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgaacgaa accgatccgg atctggaact gctgaaacgt attggcaaca acgatgcaca 121 ggcggtgaaa gaaatggtga cccgcaaact gccgcgcctg ctggcgctgg cgagccgtct 181 gctgggcgat gcggatgaag cgcgcgatat tgcgcaggaa agctttctgc gcatttggaa 241 acaggcagcg agctggcgca gcgaacaggc gcgctttgat acctggctgc atcgcgtggc 301 gctgaacctg tgctatgatc gcctgcgccg tcgtaaagaa catgtgccgg tggatagcga 361 acatgcgtgc gaagcgctgg atacccgccc ggcgccggat gaacagctgg aagcgagcgc 421 gcagagccgt cgcatggcgc aggcactgga tcagctgccg gatcgtcagc gcgaagcgat 481 tgtgctgcaa tattatcagg aactgagcaa caccgaagca gcggcactga tgcagattag 541 cgtggaagcg ctggaaagcc tgctgagccg cgcacgtcgc aacctgcgca gccatctggc 601 ggaagcgccg ggtgcggatc tgagcggccg tcgcaaaccg taatactaga gccaggcatc 661 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 721 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 781 tactagaggc gcggataaaa atttcatttg cccgcgacgg attccccgcc catctatcgt 841 tgaacccatc agctgcgttc atcagcgata ctagagaaag aggagaaata ctagatgcgt 901 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 961 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 1021 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 1081 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 1141 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 1201 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 1261 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 1321 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 1381 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 1441 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 1501 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 1561 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaacg acgaaaacta 1621 cgctgctgtc taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 1681 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 1741 gctcaccttc gggtgggcct ttctgcgttt atatactaga gcaatacgca aaccgcctct 1801 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 1861 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 1921 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 1981 atactagaga aagaggagaa atactagatg accccggaac gttttgtgca tctggcggat 2041 gcgtatggtg cagatctgca acgttggccg agcgcagaac gtgcagcagc acaggcactg 2101 ctggattgcg gcgatgcgca ggcagtggca gcgctgcgtc aggcacattg gctggatagc 2161 cagctggatc gttatcaggt gccggcaccg agcccggcac tggcacagcg tattattgcg 2221 gcagcacaac agccgggtgc accgttttgg agccgctatg caggttggct ggcaagcctg 2281 ggttgggtgg gtgtgggcct gacaggcgtg gcagcgggta tgctggcggt ggcactgagc 2341 ctgccattaa gcaccagcgc agaagcactg ccaagcgtgt ttgatcagag cgatgcagaa 2401 tttgtgctga gcattaacgc ggaagaagcg gaacagtaat actagagcca ggcatcaaat 2461 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2521 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //